Angelo Fortunato

The costs and benefits of being a chimera.

Most multicellular organisms are uniclonal. This is hypothesized to be because uniclonal organisms function better than chimeras (non–clonal organisms), owing to reduced levels of internal genetic conflict. We tested this idea using the social amoeba or slime mold Dictyostelium discoideum. When starving, the normally solitary amoebae aggregate to form a differentiated multicellular slug that migrates towards light and forms a fruiting body, facilitating the dispersal of spores. We added 107 amoebae to Petri plates containing 1, 2, 5 or 10 clones mixed together. We found an intrinsic cost to chimerism: chimeric slugs moved significantly less far than uniclonal slugs of the same size. However, in nature, joining with other clones to form a chimera should increase slug size, and larger slugs travel further. We incorporated this size effect into a second experiment by giving chimeras more cells than single clones (single clones had 106 cells, two–clone chimeras had 2 × 106 cells and so on). The uniclonal treatments then simulated a clone in a mixture that refuses to form chimeras. In this experiment, chimeras moved significantly further than the uniclonal slugs, in spite of the intrinsic cost. Thus, chimerism is costly, which may be why it evolves so seldom, but in D. discoideum the benefits of large size appear to compensate.

Co-occurrence in nature of different clones of the social amoeba, Dictyostelium discoideum

The social amoeba, Dictyostelium discoideum, produces a multicellular fruiting body and has become a model system for cell–cell interactions such as signalling, adhesion and development. However, unlike most multicellular organisms, it forms by aggregation of cells and, in the laboratory, forms genetic chimeras where there may be competition among clones. Here we show that chimera formation is also likely in nature, because different clones commonly co‐occur on a very small scale. This suggests that D. discoideum will likely have evolved strategies for competing in chimeras, and that the function of some developmental genes will be competitive. Natural chimerism also makes D. discoideum a good model organism for the investigation of issues relating to coexistence and conflict between cells.

A linear dominance hierarchy among clones in chimeras of the social amoeba Dictyostelium discoideum

Abstract Amoebae from different clones of Dictyostelium discoideum aggregate into a common slug, which migrates towards light for dispersal, then forms a fruiting body consisting of a somatic, dead stalk, holding up a head of living spores. Contributions of two clones in a chimera to spore and stalk are often unequal, with one clone taking advantage of the others stalk contribution. To determine whether there was a hierarchy of exploitation among clones, we competed all possible pairs among seven clones and measured their relative representation in the prespore and prestalk stages and in the final spore stage. We found a clear linear hierarchy at the final spore stage, but not at earlier stages. These results suggest that there is either a single principal mechanism or additive effects for differential contribution to the spore, and that it involves more than spore/stalk competition.

A new role for antennation in paper wasps (Hymenoptera,Vespidae): antennal courtship and sex dimorphic glands in antennomeres

Summary.Males of Polistes dominulus perform antennal vibrations and grasping of female antennae during pre-copulatory and copulatory phases. Male antennation plays a relevant role in mating success. In several antennomeres of males of P. dominulus and Vespa crabro, a further species in which we observed male antennation, we found secretory cells of class 1 and 3 associated to the same release site. In P. dominulus, class 3 cells with ampulla-like reservoirs and class 1 cells release their secretion through hairless multiporous areas. In V. crabro, tyloid-like structures are associated with large apodemes. The as yet unidentified secretion of these glands may act as a contact or low-volatile sex pheromone during courtship behaviour.

The Cost of Queen Loss in the Social Wasp Polistes dominulus (Hymenoptera: Vespidae)

Abstract Loss of the queen is a crisis for a social insect colony. The process of queen succession could cause increased aggression and work inefficiencies, and the new queen, if the colony can produce one, could be slow to develop mature eggs. We evaluated the cost of queen replacement in Polistes dominulus by removing the queen from a set of single-foundress colonies while leaving a control set with their queens. At 2 and 11 days after queen removal, we found that the queenless colonies had increased levels of some dominance behaviors, chewing and climbing, but not of the far more common lunging and biting. However, foraging behavior did not decrease on nests without queens as compared to nests with their original queens. Nest growth diminished as compared to control nests, as would be expected if new queens were not as competent at egg laying or if dominance behavior interfered with nurturing activities. Furthermore, replacement queens did not mate in the first 12 days after queen removal and few had mature eggs in their ovaries, though after a month most had mated and had developed ovaries. The degree of ovarian dominance of the top egg-layer over the others was also diminished at 12 days, but by a month the new queen was as dominant as control queens. The high cost of replacing the queen may indicate that workers are kept reproductively suppressed enough not to be a threat to the existing queen.

hERG1 channels modulate integrin signaling to trigger angiogenesis and tumor progression in colorectal cancer

Angiogenesis is a potential target for cancer therapy. We identified a novel signaling pathway that sustains angiogenesis and progression in colorectal cancer (CRC). This pathway is triggered by β1 integrin-mediated adhesion and leads to VEGF-A secretion. The effect is modulated by the human ether-à-go-go related gene 1 (hERG1) K+ channel. hERG1 recruits and activates PI3K and Akt. This in turn increases the Hypoxia Inducible Factor (HIF)-dependent transcription of VEGF-A and other tumour progression genes. This signaling pathway has novel features in that the integrin- and hERG1-dependent activation of HIF (i) is triggered in normoxia, especially after CRC cells have experienced a hypoxic stage, (ii) involves NF-kB and (iii) is counteracted by an active p53. Blocking hERG1 switches this pathway off also in vivo, by inhibiting cell growth, angiogenesis and metastatic spread. This suggests that non-cardiotoxic anti-hERG1 drugs might be a fruitful therapeutic strategy to prevent the failure of anti-VEGF therapy.

Fetal microchimerism and maternal health: A review and evolutionary analysis of cooperation and conflict beyond the womb

The presence of fetal cells has been associated with both positive and negative effects on maternal health. These paradoxical effects may be due to the fact that maternal and offspring fitness interests are aligned in certain domains and conflicting in others, which may have led to the evolution of fetal microchimeric phenotypes that can manipulate maternal tissues. We use cooperation and conflict theory to generate testable predictions about domains in which fetal microchimerism may enhance maternal health and those in which it may be detrimental. This framework suggests that fetal cells may function both to contribute to maternal somatic maintenance (e.g. wound healing) and to manipulate maternal physiology to enhance resource transmission to offspring (e.g. enhancing milk production). In this review, we use an evolutionary framework to make testable predictions about the role of fetal microchimerism in lactation, thyroid function, autoimmune disease, cancer and maternal emotional, and psychological health.

Alarm communication in Ropalidia social wasps

SummaryAlarm pheromones, chemical substances produced by social insects to alert the colony to threat, are the principal means by which colony defence is co-ordinated. We present the results of a study on alarm behaviour in 5 swarming species of wasps belonging to the genus Ropalidia. These species show a remarkably efficient strategy of alarm communication, including visual display and attack synchronization. We show that pheromones released from the venom gland play an important role in alarm recruitment in species belonging to the Ropalidia flavopicta group, but not in Ropalidia sumatrae. We analysed the contents of the venom reservoirs content of four of the studied species by gas chromatography-mass spectrometry. Glands were found to contain a complex mixture of volatile compounds as well as spiroacetals of higher molecular weight. Interestingly, despite all species producing similar chemical compounds from the venom gland, these were found to elicit alarm behaviour in only those species that build nest envelopes, suggesting a link between chemical release of alarm behaviour and the evolution of nest architecture in Ropalidia wasps.

Use of Dufour's gland secretion in nest defence and brood nutrition by hover wasps (Hymenoptera, Stenogastrinae).

Social wasps of the subfamily Stenogastrinae produce an abdominal secretion that is used in two distinct biological contexts. First, the secretion plays an important role in larval nutrition where it serves as a substrate in which food is placed by the adults for eventual consumption by the larvae. Second, in several species, females apply the same secretion to the substrate on which their nests are constructed, where it constitutes a sticky barrier that defends the immature brood from predation by ants. This paper describes for the first time ant guard construction behaviour of three species of stenogastrine wasps belonging to the genera Eustenogaster and Liostenogaster. The identification of compounds making up these secretions was also performed by gas chromatography-mass spectrometry. Ant guards and brood secretions were similar, with saturated and unsaturated long chain hydrocarbons and alcohols as major components. We further confirm that the glandular source of abdominal secretion is the Dufours gland. This gland contains the same hydrocarbons, and in the same proportions as ant guards and brood secretion. We discuss the fundamental importance of Dufours gland secretion in the social life of these wasps by comparing species with and without ant guards within the subfamily.

Defensive Role of Secretion of Ectal Mandibular Glands of the Wasp Polistes dominulus

Ectal mandibular glands of insects are exocrine glands localized in the anterior–lateral region of the head, close to the base of the mandibles. In social wasps, the gland is composed of secretory cells and a reservoir into which the secretion accumulates. At the time of emission the secretion flows onto a specialized cuticular area on the outside of the base of the mandibles. Secretion of Polistes dominulus is emitted only when wasps are greatly disturbed or in the presence of predators, and its function seems to be mainly defensive. Morphometric studies did not reveal any size differences between the glands of the queens and those of the workers. GC-MS analyses of the glands identified 32 compounds, mainly acids and aldehydes in the range C2–C18. The overall odor, caused by the mixture of aldehydes, is distinct. Workers do not respond strongly to the odor. The secretion probably serves as a warning signal to vertebrate predators.