Anil Tiwari

Toxicity of scorpion (Buthus tamulus) venom in mammals is influenced by the age and species

The present study was undertaken to determine the toxicity of scorpion (Buthus tamulus) venom in young and adult rats, as well as in different species of adult animals (rats, mice and guinea-pigs). The median lethal dose (LD50; mg/kg s.c.) of scorpion venom in young and adult rats was 2.2 +/- 0.21 and 1.3 +/- 0.14, respectively. The LD50 value for mice (7.2 +/- 1.35) was significantly greater than adult rats or guinea-pigs (1.14 +/- 0.08). The LD50 dose for i.v. route in anaesthetized adult rats was 95 +/- 13.2 micrograms/kg weight, which is 13 times less than that required for s.c. route. The results show that the lethality of scorpion venom in mammals differs with the age and species of the animals.

B2 kinin receptors mediate the Indian red scorpion venom‐induced augmentation of visceral reflexes via the nitric oxide cyclic guanosine monophosphate pathway

Aim:  This study was performed to delineate the kinin (receptor)‐dependent pathways in the Indian red scorpion (Mesobuthus tamulus; MBT) venom‐induced pulmonary oedema as well as the augmentation of cardio‐pulmonary reflexes evoked by phenyldiguanide (PDG).

Mesobuthus tamulus venom induces acute respiratory distress syndrome in rats involving additional mechanisms as compared to oleic acid model

The present study was undertaken to determine whether acute respiratory distress syndrome (ARDS) is produced after Mesobuthus tamulus (MBT) envenomation and compared it with oleic acid (OA)-induced ARDS. The trachea, jugular vein and femoral artery were cannulated in anesthetized adult rats. Lethal dose of MBT venom (5 mg/kg) or OA (75 μL) was administered intravenously and the time-dependent changes in respiratory frequency (RF), heart rate (HR) and mean arterial pressure (MAP) were recorded. Minute ventilation (MV) and the PaO2/FiO2 (P/F) ratio were also determined. At the end lungs were excised, one lung was used for histopathological examination and the other was used for determination of pulmonary water content physically. MBT venom or OA produced hypoxemia, pulmonary pathology (alveolar damage, infiltration of inflammatory cells, capillary damage and exudation) and pulmonary edema implicating for ARDS. However, the hypoxemia in MBT venom group was associated with decreased MV, apnea/bradypnea, and bradycardia whereas, in OA group it was seen with increased MV, tachypnea, and tachycardia. Lack of effect of hypoxemic drive on RF/MV or HR in MBT venom group unlike OA group, suggests the involvement of medullary centers. The present results demonstrate that MBT venom produces ARDS. However MBT venom-induced ARDS involves pulmonary as well as extrapulmonary mechanisms.

Morphine blocks the Mesobuthus tamulus venom-induced augmentation of phenyldiguanide reflex and pulmonary edema in anesthetized rats.

Objective: Pulmonary edema, a manifestation of scorpion envenomation syndrome, is attributed to cardiogenic or noncardiogenic factors. Morphine is a drug used for cardiogenic pulmonary edema and its effect on Mesobuthus tamulus (MBT) venom-induced changes is not known. Therefore, we hypothesized that morphine blocks the MBT venom-induced augmentation of phenyldiguanide (PDG) reflex and pulmonary edema. Materials and Methods: Experiments were performed on anesthetized adult female rats. Trachea and jugular vein were cannulated, and the electrocardiographic potentials were recorded by connecting needle electrodes in limb lead II configuration. PDG (10 ΅g/kg, IV, bolus injection) responses were elicited by bolus injection initially, after saline/morphine (1 mg/kg) and after injecting MBT venom (100 μg/kg). The time-response area of the PDG-induced bradycardiac response after treatment was calculated as % of the initial PDG response area. At the end of experiments, lungs were excised for determination of pulmonary water content. Results: PDG produced bradycardiac response that lasted for >60 s. MBT venom augmented the PDG reflex response by 2.5 times. In morphine pretreated group, augmentation of bradycardiac response induced by MBT venom was absent. MBT venom increased the pulmonary water content, and the increase was absent in morphine pretreated animals. Conclusion: The results reveal that morphine prevents the MBT venom-induced augmentation of PDG reflex response and pulmonary edema. Thus, morphine can be useful in scorpion envenomation syndrome associated with pulmonary edema.

Role of serotonergic mechanism in gastric contractions induced by Indian Red Scorpion (Mesobuthus tamulus) venom

Aim: Gastric dysfunctions are commonly seen after scorpion envenomation, and the underlying mechanisms are not clear. Therefore, the present study was undertaken to investigate the effect of Indian red scorpion (Mesobuthus tamulus, MBT) venom on gastric fundus muscle contraction and the underlying mechanisms involved. Materials and Methods: In vitro isometric contraction was recorded from gastric fundus muscle strips on a chart recorder. The tissue was exposed to different concentrations of serotonin or crude MBT venom. The contractile responses to venom were expressed as the percentage of maximum contraction produced by serotonin at the beginning of each experiment. The contractile responses to 1.0 μg/ml of crude MBT venom were ascertained in the absence or presence of serotonin antagonist, methysergide. Results: Serotonin produced concentration-dependent fundus contractions (0.004–4.0 μM), and maximum contractile response was observed at 4.0 μM of serotonin. Hence, the contractile response obtained at 4.0 μM of serotonin was taken for normalization. The crude MBT venom (0.1–1.0 μg/ml) produced a concentration-dependent increase in fundus contractions (as % of maximum fundus contraction produced by serotonin at 4.0 μM). The maximum response was observed at 1.0 μg/ml of crude venom and a further increase in the concentration, up to 3.0 μg/ml, did not increase the response. In a separate series of experiments, pre-treatment with methysergide (1.0 μM) significantly attenuated the contractile response elicited by the venom (1.0 μg/ml) (P<0.05) and blocked the serotonin (4.0 μM) response. Conclusion: The results suggest that the crude MBT venom produces gastric fundus contractions by partially involving serotonin.