Aniversari Apriana

Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens

The rice transformation technology is not only provides valuable methods for the introduction of useful genes into rice plant to improve important agronomic traits, but also helps in studying gene function and regulation based on rice genome sequence information. Knockout of genes by insertional mutagenesis is a straightforward method to identify gene functions. One of the methods to develop rice mutants is through genetic transformation mediated by Agrobacterium using activation tagging by Ac-Ds system. A study was done with an objective to obtain mutant rice of local tropical japonica cv. Asemandi through genetic trans-formation mediated by Agrobacterium tumefaciens. The transformation was conducted using Agrobacterium vector with the strain of Agl-1 containing activation tag construct. The result of experiment showed that it has been obtained 17 independent line (304 plants) transgenic Asemandi containing activation tag construct. These starter lines will be used as materials to develop several generations of stabil rice mutant through selfing.

Induksi Kalus dan Regenerasi Beberapa Genotipe Gandum (Triticum aestivum L.) secara In Vitro

Callus Induction and In Vitro Plant Regeneration of Wheat Genotypes (Triticum aestivum L.). Atmitri Sisharmini, Aniversari Apriana, and Sustiprijatno. Development of a reliable in vitro plant regeneration procedure for wheat is a prerequisite for its improvement by genetic transformation. The purpose of this study was to obtain methods of callus induction and regeneration of wheat genotypes. This experiment was conducted at ICABIOGRAD. Immature embryos from four wheat genotypes, ie Perdix, Naxos Wew, Combi and Fasan were used to induce callus formation and regeneration rate of callus. For the preparation of callus induction medium, MS-L7 basal medium was supplemented with combination of growth regulators 2,4 dichlorophenoxy acetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid (picloram). While, plant regeneration medium was prepared using MS basal medium supplemented with combination of three growth regulators i.e. IAA, BAP and kinetin. The results showed that genotype, in vitro culture medium and growth regulators played a dominant role in callus induction and plantlet regeneration. All the 4 genotypes responded positively to callus induction, however, variability was observed not only among the genotypes but also within callus induction medium used. The best induction medium was the MS-L7 basal medium supplemented with combination of phytohormon 4 mg/l 2,4-D + 2 mg/l picloram (GIK-3) which showed 100% callus induction frequency. Whereas, the best regeneration medium was shown by MS basal medium with combination of phytohormon 1.5 mg/l BAP dan 0.5 mg/l kinetin (RG3). Regarding plant regeneration, Perdix was the most responsive genotype to be regenerated with regeneration frequency of 57.33%. The successfully acclimatized planlets in greenhouse were obtained from Perdix and Naxos Wew genotypes. These results will potentially facilitate genetic transformation research of wheat in Indonesia.