Anna M. Kordowiak

Morphology of the Golgi Apparatus in Streptozotocin-Diabetic Rat Liver

The altered morphology of the Golgi apparatus in streptozotocin-diabetic rat liver is described in situ and in preparations of the Golgi-membrane-enriched fraction. The main differences, as compared with controls, are a diminished Golgi apparatus area, the rounding of stacks of cisternae and the absence of secretory vacuoles with VLDL granules. These findings are discussed in view of biochemical alterations found previously in the Golgi-membrane-enriched fraction isolated from streptozotocin-diabetic rat liver.

Biochemical and Morphological Alterations in Rat Liver Golgi Complexes After Treatment with Bis(maltolato)oxovanadium(IV) [BMOV] orMaltol Alone

Summary Oral treatment with maltol or bis(maltolato)oxovanadi- um(IV) [BMOV] alters the biochemical activity of the rat liver Golgi marker enzyme, i.e., galactosyltrans- ferase (GalT), and the organelle morphology in a relatively short time. Four groups of rats were investigated: control (C), treated with BMOV for 2 days (pVC), treated with BMOV for 7 days (C+V), and treated with maltol alone for 7 days (C+M). All drugs were administered as drinking solutions. These conditions were used, because normalization of galactosyltransferase activity (GalT) and morphology of rat liver Golgi complexes were previously found by us in streptozotocin-induced diabetes. In this paper, we present the influence of BMOV or maltol alone (as a vanadium ligand in BMOV compound) on rat liver Golgi complexes. The lowest statistically significant enzyme activity, in comparison with three other groups of rats (p

Influence of Insulin on Galactosyl Transferase Activity and Morphology of the Liver Golgi Apparatus in Control and Streptozotocin-Diabetic Rats*

Summary Insulin does not influence either the yield of the liver Golgi membrane-enriched fraction or the activity of its UDP-Gal→ G1cNAc transferase activity. Morphological studies show that the hormone slightly stimulates the secretory action of the Golgi apparatus in normal rat liver and prevents the formation of rounded cisternae of this organelle in the streptozotocin-diabetic rat liver. Other morphological alterations of the liver Golgi apparatus in streptozotocin-diabetic rats are not improved by insulin treatment.