Anna Nikoghosyan

Cell bathing medium as a target for non thermal effect of millimeter waves

Non thermal (NT) effect of direct radiation 4 Hz-modulated 90–160 GHz of Millimeter Waves (MMW) and preliminary MMW-treated physiological solution (PS) influence were studied on snail isolated neuron, rats brain tissue hydration and skin penetration. It was shown that the 4 Hz-modulated low intensity 90–160 GHz MMW direct radiation and MMW-treated PS leads to on single neuron shrinkage, skin and brain tissue dehydration. On the basis of obtained data it was suggested that the cell bathing aqua medium serve as a target through which the NT effect of MMW on cell hydration is realized. The MMW-induced brain tissue dehydration can considering as consequence of MMW-induced skin water structural changes leading to unknown messenger formation able to modulate the brain cell hydration. The extrasensitivity of cell hydration to low intensity of MMW radiation allow to recommend cell hydration as a cellular marker for estimation of the NT biological effect of MMW on cells and organisms.

Age-dependent effect of static magnetic field on brain tissue hydration

Abstract Age-dependent effect of Static Magnetic Field (SMF) on rats in a condition of active and inactive Na+/K+ pump was studied for comparison of brain tissues hydration state changes and magnetic sensitivity. Influence of 15 min 0, 2 Tesla (T) SMF on brain tissue hydration of three aged groups of male albino rats was studied. Tyrode’s physiological solution and 10−4 M ouabain was used for intraperitoneal injections. For animal immobilization, the liquid nitrogen was used and the definition of tissue water content was performed by tissue drying method. Initial water content in brain tissues of young animals is significantly higher than in those of adult and aged ones. SMF exposure leads to decrease of water content in brain tissues of young animals and increase in brain tissues of adult and aged ones. In case of ouabain-poisoned animals, SMF gives reversal effects on brain tissue’s hydration both in young and aged animals, while no significant effect on adults is observed. It is suggested that initial state of tissue hydration could play a crucial role in animal age-dependent magnetic sensitivity and the main reason for this could be age-dependent dysfunction of Na+/K+ pump.

The Quantum-Mechanical Sensitivity of Cell Hydration in Mammals

The elucidation of the mechanism on the biological effects of weak chemical and physical factors on cells and organism is one of the modern problems in life sciences. According to the Receptor Theory of Prof. Bernard Katz the impact of the biological substances on cells is realized through the activation of ligand-gated ion channels in the membrane. However, this theory doesn’t provide a satisfactory explanation on the similar biological effects of extremely low concentrations of different chemical substances, which are unable to activate the ionic channels in the membrane and have non-linear dose-dependent effect on cells. Previously we have suggested that the metabolic control of cell hydration serves as a universal quantum-mechanical sensor for different weak physical and chemical signals. For supporting this hypothesis, in this article the comparative study of the effects of low concentrations of both cold (non-radioactive) and [3H]-ouabain (specific inhibitor for Na+/K+-ATPase) on the hydration in different tissues of rats has been performed. The obtained data have shown that cold and [3H]-ouabain have different effects on cell hydration and such a difference depends on the initial metabolic state of tissues. On the basis of our previous and present results it is suggested that such a quantum-mechanical sensitivity of cell hydration is realized through the cyclic-nucleotides-dependent Na+/Ca2+ exchange, having a crucial role in the metabolic regulation of cell hydration.