Anna Pietryczuk

The influence of traumatic acid on the growth and metabolite content of the green alga Chlorella vulgaris Beijerinck

The influence of traumatic acid on the growth and metabolite content of the green alga Chlorella vulgaris Beijerinck The effect of exogenous traumatic acid, (2E)-dodecene-1,12-dioic acid (TA), at concentrations of 10-6 - 10-3 M on the growth and development of the unicellular green alga Chlorella vulgaris was examined over 7 days. TA plays an important role in algal growth and development. Treatment with 10-5 M TA resulted in the greatest increase in cell numbers, by 77%, chlorophyll a content, by 126%, chlorophyll b, by 45%, total carotenoids, by 65%, monosaccharides, by 111%, and water-soluble proteins, by 37%, in relation to the control. After three days of treatment with 10-5 M TA cultures were analysed by SDS-PAGE, which showed the presence of new polypeptides with molecular weights 13-103 kDa.

Effect of traumatic acid on antioxidant activity in Chlorella vulgaris (Chlorophyceae)

The present study was undertaken to test the influence of exogenously applied traumatic acid (TA) upon the activity of several antioxidant enzymes as well as lipid and protein peroxidation in green algae Chlorella vulgaris. Treatment with TA in concentration range of 10−6–10−5 M resulted in an increase of antioxidant enzyme (sodium dismutase, catalase, ascorbate peroxidase, NADH peroxidase, glutathione reductase) activity. Moreover, TA suppressed lipid peroxidation and oxidative destruction of proteins belonging to the SH groups. This data suggest that TA plays an important role in the metabolism of C. vulgaris and probably in its high ability to adapt to various environmental stress factors.

The effect of traumatic acid on the growth, metabolite content and antioxidant activity in Wolffia arrhiza (L.) Wimm. (Lemnaceae)

The research presented here was conducted in order to determine the influence of exogenous traumatic acid (TA) on the growth, metabolism, and antioxidative activity of vascular water plant, Wolffia arrhiza Wimm. The research was concerned with TA influences, in concentrations of 10−8 M − 10−4 M, on fresh W. arrhiza, and on primary metabolites, such as monosaccharides, proteins, chlorophylls a and b, and carotenoids. It was determined that TA causes a substantial increase in these metabolites compared to the control, especially at concentrations of 10−7 − 10−6 M. Polyacrylamide gel electrophoresis of proteins (SDS-PAGE) was conducted in order to specify in more detail the qualitative changes in proteins, whose synthesis is stimulated by TA. Under the influence of TA W. arrhiza cells saw an induction of de novo synthesis of 3 proteins with molecular weights of 10, 58, and 90 kDa. It was proven that 10−7 − 10−6 M concentrations of TA also increase photosynthesis intensity and the activity of antioxidative enzymes (ascorbate peroxidase, catalase, glutathione reductase, and superoxide dismutase). In cells treated with exogenous TA, lipids’ peroxidation decreases (expressed as a drop in malonyl dialdehyde) and sulfhydryl (SH) groups in proteins increase. Based on our research, TA plays an important role in the regulation of growth and metabolism in W. arrhiza. Our results also show that TA possibly participates in the activation of antioxidant enzymes and its probable participation in the metabolic responses of lower water plants to oxidative stresses.

The effect of sodium amidotrizoate on the growth and content of various metabolites in the green alga Chlorella vulgaris Beijerinck

The effect of sodium amidotrizoate on the growth and content of various metabolites in the green alga Chlorella vulgaris Beijerinck The aim of this work is to detect how 3,5-diacetamido-2,4,6-triiodosodium benzoate (sodium amidotrizoate), a synthetic derivative of the anti-auxin triiodonezoic acid (TIBA), influences the growth and content of primary metabolites in the green alga Chlorella vulgaris Beijerinck. It strongly suppressed cell number by 6-20%, chlorophyll a by 6-14%, chlorophyll b by 20-24%, total carotenoids by 7-13% and monosaccharides by 7-8% at the concentration of 10-4 M, in comparison to the control. On the other hand, sodium amidotrizoate applied at lower concentrations (10-7 - 10-6 M) acted as a weak stimulator of analyzed biochemical compounds in algal cells. No statistically significant effect was detected with the application of sodium amidotrizoate at a concentration of 10-8 M. It seems that sodium amidotrizoate plays an important role in algal growth and development and probably possesses anti-auxin activity, like TIBA.