Anna Rogiewicz

Low-fiber canola. Part 1. Chemical and nutritive composition of the meal.

The objective of the current study was to evaluate the chemical and nutritive composition of meals derived from a newly developed yellow-seeded Brassica napus canola and the canola-quality Brassica juncea . In comparison with its conventional black-seeded counterpart, meal derived from yellow-seeded B. napus canola contained more protein (49.8 vs 43.8% DM), more sucrose (10.2 vs 8.8% DM), and less total dietary fiber (24.1 vs 30.1% DM). B. juncea canola showed intermediate levels of protein, sucrose, and dietary fiber (47.4, 9.2, and 25.8%, respectively). The reduction in fiber content of yellow-seeded B. napus canola was a consequence of a bigger seed size, a lower contribution of the hull fraction to the total seed mass, and a lower content of lignin with associated polyphenols of the hull fraction. The meal derived from yellow-seeded B. napus canola would appear to have quality characteristics superior to those from black-seeded B. napus or yellow-seeded B. juncea.

Low-fiber canola. Part 2. Nutritive value of the meal.

The nutritive value of meals derived from black- and yellow-seeded Brassica napus and canola-quality Brassica juncea was determined with broiler chickens and young turkeys. A higher apparent ileal digestibility of total amino acids was observed in chickens fed diet containing yellow-seeded B. napus than in those fed conventional black-seeded B. napus or canola-quality B. juncea (88.8 vs 83.4 and 84.2%, P < 0.05). Metabolizable energy (AME(n)) contents for yellow- and black-seeded B. napus and B. juncea as determined with broiler chickens were 2190, 1904, and 1736 kcal/kg DM, respectively. In the turkey assay, the AME(n) values for yellow- and black-seeded B. napus and B. juncea canola averaged 2166, 2007, and 1877 kcal/kg DM, respectively. Multicarbohydrase enzyme addition to broiler chicken diets increased energy utilization (from 1943 to 2249 kcal/kg DM, on average), with the most pronounced effect observed for B. juncea canola (from 1736 to 2356 kcal/kg DM).

The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets

A study was conducted to determine if amylase and protease addition would improve nutrient digestion during the first 2 wk of growth. The experimental treatments included a control corn-soybean meal-based diet and diets supplemented with either amylase or amylase plus protease. No effect of enzyme supplementation was observed on BW gain and feed conversion ratio. This was corroborated by similar ileal starch and protein digestibility values, which averaged 96.8, 96.8, and 96.9% and 83.9, 80.1, and 79.6%, respectively, for the control and for the amylase or amylase plus protease supplemented diets. Total tract digestibility of starch averaged 97.8, 97.7 and 97.7% for the 3 diets and was followed by a similar diet with AMEn values of 3,129, 3,129, and 3,106 kcal/kg. In another study, a 2(3) factorial arrangement of 8 dietary treatments was used to evaluate the effect of corn particle size (conventional or coarse vs. fine) and the addition of a nonstarch polysaccharide enzyme, amylase, or both on growth performance and nutrient utilization of broiler chickens from 1 to 21 d of age. Chickens fed a diet containing a conventionally ground corn (geometric mean diameter of 736 µm) showed higher (P < 0.001) BW gain (808 vs. 750 g/bird) and lower feed conversion ratio (1.27 vs. 1.32) than those consuming a fine corn-containing diet (geometric mean diameter of 482 µm). This was further substantiated by a lower AMEn content (2,852 vs. 2,972 kcal/kg). Addition of amylase had no effect on growth performance of chickens fed a conventional corn-containing diet, but improved BW gain, feed conversion ratio, and diet AMEn in those fed the finely ground corn, possibly due to increased starch digestion in the upper gut. Addition of nonstarch polysaccharide enzymes was effective for both diets, with the most pronounced effects observed in feed conversion ratio for the conventional corn-containing diet (1.27 vs. 1.23) and BW gain (750 vs. 789 g/bird) for the fine corn-containing diet. This was followed by the same magnitude of difference in diet AMEn content, which increased from 2,972 to 3,042 and 2,852 to 3,009 kcal/kg following enzyme addition.

Feeding flaxseed enhances deposition of omega-3 fatty acids in broiler meat portions in different manner

Feeding a diet containing 12% flaxseed for 36 d significantly increased the n-3 fatty acid deposition in broiler meat. Based on this study, consuming 100 g of such fatty acid-enriched breast meat, whole leg or wing, a total of 0.2, 1.6 and 2.0 g of n-3 fatty acids, including 10.4, 20.3 and 25.3 mg of docosahexaenoic acid, respectively, would be provided by each portion, which offers consumers an alternative to enhance their daily n-3 fatty acid intake.Key words: Flaxseed, n-3 fatty acid, broiler chicken

Effect of Microwave Treatment on the Efficacy of Expeller Pressing of Brassica napus Rapeseed and Brassica juncea Mustard Seeds

A study was conducted to evaluate the effect of microwave heating on the efficacy of expeller pressing of rapeseed and mustard seed and the composition of expeller meals in two types of Brassica napus rapeseed (intermediate- and low-glucosinolate) and in Brassica juncea mustard (high-glucosinolate). Following microwave treatment, the microstructure of rapeseed using transmission electron microscopy showed a significant disappearance of oil bodies and myrosin cells. After 6 min of microwave heating (400 g, 800 W), the oil content of rapeseed expeller meal decreased from 44.9 to 13.5% for intermediate-glucosinolate B. napus rapeseed, from 42.6 to 11.3% for low-glucosinolate B. napus rapeseed, and from 44.4 to 14.1% for B. juncea mustard. The latter values were much lower than the oil contents of the corresponding expeller meals derived from the unheated seeds (i.e., 26.6, 22.6, and 29.8%, respectively). Neutral detergent fiber (NDF) contents showed no differences except for the expeller meal from the intermediate-glucosinolate B. napus rapeseed, which increased from 22.7 to 29.2% after 6 min of microwave heating. Microwave treatment for 4 and 5 min effectively inactivated myrosinase enzyme of intermediate-glucosinolate B. napus rapeseed and B. juncea mustard seed, respectively. In low-glucosinolate B. napus rapeseed the enzyme appeared to be more heat stable, with some activity being present after 6 min of microwave heating. Myrosinase enzyme inactivation had a profound effect on the glucosinolate content of expeller meals and prevented their hydrolysis to toxic breakdown products during the expelling process. It appeared evident from this study that microwave heating for 6 min was an effective method of producing expeller meal without toxic glucosinolate breakdown products while at the same time facilitating high yield of oil during the expelling process.

Standardized ileal digestible amino acid contents of canola meal from Canadian crushing plants for growing pigs

Studies have shown that there are variations in the chemical and nutritive composition of canola meal (CM) produced in Canada, some of which may result from differences among the canola seed crushing plants. Therefore, it is necessary to determine the variability in the nutritive value of CM as its end users desire increased meal consistency and more information on any potential variability in AA digestibility. Thus, 18 ileal cannulated barrows (average initial BW = 23.3 kg) were used to determine the apparent (AID) and standardized (SID) ileal digestibility of N and AA and standardized ileal digestible N and AA contents in CM from 6 processing plants in Canada. Two processing plants provided CM in both mash and pellet forms, whereas the other 4 provided it only in mash form, giving a total of 8 CM samples. The barrows were fed 9 cornstarch-based diets. The first 8 diets were formulated to contain the CM samples as the only source of AA. The ninth diet was a casein-cornstarch diet to determine ileal endogenous AA losses. All diets contained 0.3% titanium dioxide as an indigestible marker. Pigs were housed individually in pens and were fed the 9 diets in a completely randomized design for 3 periods to give 6 pigs per diet. Each experimental period lasted for 7 d, consisting of 5 d of adaptation and 2 d of ileal digesta collection at 2-h intervals from 0800 to 2000 h on d 6 and 7. There was an effect ( < 0.05) of CM source on 1) the AID of N, Met, Cys, Thr, Val, Gly, and Ser; 2) the SID of N, Met, and Cys; and 3) the standardized ileal digestible content of all AA. Pelleting reduced ( < 0.05) the standardized ileal digestible content of Arg, Ile, Glu, and Gly in 1 of the CM samples tested and of all AA except His in the other CM sample. The SID values for Arg, Lys, Met, and Thr averaged 87.2%, 78.2%, 84.5%, and 74.1%, respectively. The standardized ileal digestible contents of Arg, Lys, Met, and Thr averaged 2.10%, 1.71%, 0.50%, and 1.01%, respectively. In conclusion, there were variations in the AID, SID, and standardized ileal digestible AA contents of CM among crushing plants in Canada.

Effects of canola meal source on the standardized ileal digestible amino acids and apparent metabolizable energy contents for broiler chickens

&NA; Two studies were conducted to determine the effect of canola meal source on the standardized ileal digestible amino acids (AA) and nitrogen‐corrected apparent metabolizable energy (AMEn) contents of canola meal (CM) from 6 processing plants in Canada. Two plants provided CM in both mash and pellet forms, while the other 4 provided CM in mash form only, giving a total of 8 samples. In the standardized ileal digestibility (SID) assay, 280 birds were housed 7 per cage and fed a commercial starter diet from 1 to 14 d of age followed by the test diets from 15 to 21 d. Diets were formulated to contain CM as the only source of protein. Chromic oxide was included in all diets as an indigestible marker. Diets were randomly assigned to 5 cages of 7 birds. On d 21, birds were euthanized by CO2 asphyxiation and digesta samples were collected from the terminal ileum for SID of AA. The SID values were calculated using ileal endogenous AA losses determination. In the AMEn assay, 330 birds were housed 6 per cage and fed a commercial starter diet from 1 to 14 d of age followed by the experimental diets from 15 to 19 d. The 8 experimental diets contained 30% of CM and 70% of a corn‐soybean meal‐based diet. There were differences (P < 0.05) among CM samples in the standardized ileal digestible content of all AA. Standardized ileal digestible contents of Arg, Lys, Met, and Thr averaged 2.17, 1.74, 0.53, and 1.03%, respectively. Pelleting reduced (P < 0.05) the standardized ileal digestible content of all AA in CM from one of the plants. There were differences (P<0.05) among CM samples in the AMEn contents with values ranging from 1,590 to 2,041 kcal/kg DM. In conclusion, there was effect of CM source on the standardized ileal digestible AA and AMEn contents. Regression equations for predicting standardized ileal digestible contents of selected AA from a simple measure of acid detergent fiber (ADF) were developed.

Fractionation of canola meal using sieving technology

Abstract: The present study explored the potential for the production of canola meal (CM) fractions of different chemical and nutritive composition. Three meals from conventional black- and yellow-seeded Brassica napus and Brassica juncea canola were subjected to sieving. The use of sieves from 250 to 600 µm resulted in the production of fractions fine 1, fine 2, medium, and coarse. When compared with the parent meals, the content of total dietary fiber of fractions fine 1 and 2 decreased from 300 to 214 and 267 g kg−1 for conventional CM, 270 to 216 and 234 g kg−1 for B. napus yellow, and 255 to 153 and 187 g kg−1 for B. juncea meal. Crude protein increased from 368 to 420 and 396 g kg−1 for conventional CM, 410 to 436 and 430 g kg−1 for yellow CM, and 423 to 479 and 468 g kg−1 for B. juncea meal. The effects of three parent meals and their respective fractions fine 1 and 2 were evaluated in a growth performance experiment with broiler chickens. There was no significant effect of CM diets on growth performance indicating that CM and its low-fiber fractions could effectively replace soybean meal in diets for young poultry.

Assessment of the minimal available phosphorus needs of pullets during the pre-laying period

Abstract Dietary phosphorus (P) oversupply is costly to the poultry industry and represents a point source of excessive P discharge to the environment via manure application to soils. The current study was conducted to investigate the effects of dietary available P (AP) level on growth performance, plasma biochemistry, bone characteristics, and P flow in order to assess minimal AP needs of pullets during the pre‐lay period. A total of 192 Lohmann pullet chicks were randomly allocated to one of 4 treatments with 8 replicate cages of 6 birds per cage. The pullets of Low (L; the lowest AP regimen) were fed diets in a sequence of 0.200, 0.175, and 0.150% AP for the age period of 0 to 4 wk, 4 to 8 wk, and 8 to 16 wk, respectively. The AP level was increased by an increment of 0.1% in each phase in Low‐Medium (LM), Medium‐High (MH) and High (H), thus making H (control) consisting of 0.500, 0.475, and 0.450% AP. Overall, there were no main effects of treatment on body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). However, compared with the H treatment, BWG or FI of the L treatment was lower during the first 8 wk post‐hatch, but became comparable or higher over the last 8 wk (P < 0.05); FCR of L birds was greater over wk 0 to 4 but became smaller over wk 8 to 16 (P < 0.05). Plasma P was lower in L than H treatments at wk 8 but turned comparable at wk 16 (P < 0.001). Bone characteristics were not different for pullets fed various AP regimens. Total P intake and excretion were reduced with lowering dietary AP (P < 0.001), but its retention percent was not affected. The results indicated that the lowest AP regimen (0.200‐0.175‐0.150%) in the current study was adequate to support healthy growth and development of pre‐lay pullets, while reducing P excretion in manure.

Assessment of the minimal available phosphorus needs of laying hens: Implications for phosphorus management strategies

ABSTRACT The oversupply of dietary phosphorus (P) leads to increased feed costs and discharge of excessive P to the environment, thus directly impacting the sustainability of egg production practices. The present study was conducted to better define the minimal available P needs of laying hens. Fifty‐six Lohmann white laying hens were individually caged and fed one of 7 diets with graded levels of available P (0.15, 0.20, 0.25, 0.30, 0.35, 0.40, or 0.45%) for 12 weeks. Records were maintained for body weight, feed intake, and egg production during the experimental period. Blood and egg samples were collected and digestibility studies conducted at wk 6 and 12 of the experiment. At the end of the experiment, tibia characteristics and expression of the P transporters in the small intestine and kidney were determined. Lowering dietary available P from 0.45 to 0.15% generally reduced plasma P concentrations (P < 0.01), but hen productive performance, plasma calcium, parathyroid hormone and other constituents, tibia bone mineral density and content, tibia ash percentage, and mRNA abundance of the small intestine, and kidney type II sodium/phosphate cotransporter were not different among treatments. Specific gravity and eggshell thickness tended to increase with reducing dietary P (P < 0.05). Total P intake, excretion, and retention net amount decreased (P < 0.01) with reducing dietary P, but its retention rate (of intake) remained unchanged. Furthermore, changing dietary P did not affect calcium retention rate and net deposition of total P and calcium in eggs. These data indicate that reducing dietary available P up to 0.15% is adequate to maintain health and performance of layers. As such, this minimal available P estimate should serve as a benchmark for the assessment of P contents of commercial laying hen rations, with the goal of enhancing the sustainability of egg production.