Annabel G. Liebelt

Autoregulation of Adipose Tissue Mass in the Mouse

Summary Syngeneic adipose tissue grafts showed evidence of necrosis or lack of vascularization in intact, adult (BALB/c/Ki x Ce/Ki) F1 hybrid mice between 2 and 9 months after transplantation. One or more fat depots were surgically removed to produce a deficit in the total adipose tissue mass, and this resulted in an increased number of vascularized and viable adipose tissue grafts in similar genetic hosts during a comparable period. The anatomically dispersed fat depots appear to be under some form of self-regulation and integrated into a total adipose tissue mass.

Genetic Susceptibility to Goldthioglucose-Induced Obesity in Mice.

Summary Five inbred strains of mice (C58, CBA, RIII, DBA and BALB/C) and 5 F1 hybrid combinations were studied with respect to effects of single injections of various concentrations of goldthioglucose on body weight gain and lethal toxicity. Gain in body weight and mortality following a single injection of goldthioglucose was dependent on strain of mice and amount injected. In general, there was a greater increase in weight gain when larger doses of goldthioglucose were administered. The C58, RIII, DBA and BALB/C strains of mice showed a relatively variable weight gain and mortality as compared to the CBA strain over the dose range of administered goldthioglucose. Mice of the latter strain were unique in that they showed a uniform response with respect to these 2 parameters. The F1 hybrid mice showed a weight gain response and a LD/100 dose comparable to that of the CBA strain.

INFLUENCE OF TESTIS ON INDUCTION OF OVARIAN TUMORS OF MICE BY X-RAYS

Summary When irradiated ovaries were grafted into intact as well as castrated male Balb/c mice, ovarian tumorigenesis proceeded. Functional normal ovarian grafts into previously irradiated Balb c females inhibited tumorigenesis in the irradiated ovary. It appears that irradiation-induced ovarian tumorigenesis in this inbred strain is inhibited almost completely by ovarian, but only to a minor degree by testicular secretion.

The direct effect of pituitary isografts on mammary gland development in the mouse.

Summary A single pituitary gland was transplanted adjacent to the mammary gland of the right inguinal region in intact and ovariectomized Strong A mice. Lobuloalveolar and duct development occurred locally around the pituitary graft in intact hosts while duct growth and minimal endbud development were observed in the glandular tissue adjacent to the graft in ovariectomized hosts. These data indicate that the ovary is necessary for the maximal response of mammary tissue to the direct, local effect of a pituitary isograft in Strong A mice.