Anne Gallez
University of Liège
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Anne Gallez.
Journal of Pharmaceutical and Biomedical Analysis | 2017
Gwenaël Nys; Anne Gallez; Miranda G.M. Kok; Gaël Cobraiville; Anne-Catherine Servais; Géraldine Piel; Christel Pequeux; Marianne Fillet
&NA; Quantitative bioanalysis and especially pharmacokinetic studies are challenging since only low volumes of biological material are available and low concentrations (ng/ml) are often expected. In this context, volumetric absorptive microsampling (VAMS) devices were developed to accurately collect 10 or 20 &mgr;l of whole blood from tested subjects. In this study, we present the development and validation of ultra‐high performance liquid chromatography coupled to tandem mass spectrometry method after VAMS sampling for the quantitation of estetrol (E4), a potentially new medicine for hormone replacement, contraception and osteoporosis therapies. Interestingly, a very simple sample preparation procedure was developed without any derivatization step. Even if lack of sensitivity is a common consideration when using negative ionization mode, we demonstrated in this work that an excellent sensitivity could be reached by carefully optimizing the nature and concentration of the mobile phase additive. After the optimization of every experimental parameter, the stability, selectivity, trueness, precision and accuracy of the final method were successfully demonstrated. In addition, the excellent performances of the method were confirmed by two independent proof‐of‐concept pharmacokinetic studies of E4 after VAMS collection in a murine model. Graphical abstract Figure. No caption available. HighlightsA UHPLC–MS/MS method is proposed for the analysis of estetrol without derivatization.NH4F as LC additive is used to reach needed sensitivity in negative mode.An innovative microsampling technique is used to collect 10 &mgr;l of blood from mice.The method was successfully validated for the quantitation of estetrol.A pharmacokinetic study is performed as proof‐of‐concept.
Endocrinology | 2016
Silvia Blacher; Céline Gérard; Anne Gallez; Jean-Michel Foidart; Agnès Noël; Christel Pequeux
The assessment of rodent mammary gland morphology is largely used to study the molecular mechanisms driving breast development and to analyze the impact of various endocrine disruptors with putative pathological implications. In this work, we propose a methodology relying on fully automated digital image analysis methods including image processing and quantification of the whole ductal tree and of the terminal end buds as well. It allows to accurately and objectively measure both growth parameters and fine morphological glandular structures. Mammary gland elongation was characterized by 2 parameters: the length and the epithelial area of the ductal tree. Ductal tree fine structures were characterized by: 1) branch end-point density, 2) branching density, and 3) branch length distribution. The proposed methodology was compared with quantification methods classically used in the literature. This procedure can be transposed to several software and thus largely used by scientists studying rodent mammary gland morphology.
Journal of Mammary Gland Biology and Neoplasia | 2017
Céline Gérard; Anne Gallez; Charline Dubois; Pierre Drion; Philippe Delahaut; Etienne Quertemont; Agnès Noël; Christel Pequeux
Estrogens are the subject of intensive researches aiming to elucidate their mechanism of action on the various tissues they target and especially on mammary gland and breast cancer. The use of ready-to-use slow releasing devices to administer steroids, especially estrogens, to small experimental animals remains the method of choice in terms of animal well-being and of safety for both the researcher and the animal. In this study, we evaluated and compared, in vitro and in vivo, the release kinetic of estradiol (E2) over sixty days from two different slow-releasing systems: the matrix pellet (MP) and the reservoir implant (RI). We compared the impact of these systems in three E2-sensitive mouse models : mammary gland development, human MCF7 adenocarcinoma xenograft and mouse melanoma progression. The real amount of E2 that is released from both types of devices could differ from manufacturer specifications due to inadequate release for MP and initial burst effect for RI. Compared to MP, the interindividual variability was reduced with RI thanks to a superior control of the E2 release. Depending on the dose-dependent sensitivity of the physiological or pathological readout studied, this could lead to an improvement of the statistical power of in vivo experiments and thus to a reduction of the required animal number. Altogether, our data draw attention on the importance to adequately select the slow-releasing device that is the most appropriated to a specific experiment to better fulfill the 3Rs rule (Replacement, Reduction, Refinement) related to animal welfare and protection.
Archive | 2017
Anne Gallez; Silvia Blacher; Françoise Lenfant; Jean-François Arnal; Myriam Polette; Philippe Birembaut; Agnès Noël; Jean-Michel Foidart; Christel Pequeux
Archive | 2017
Charline Dubois; Silvia Blacher; Irina Primac; Melissa García Caballero; Anne Gallez; Natacha Rocks; Christel Pequeux; Didier Cataldo
Archive | 2017
Anne Gallez; Silvia Blacher; Céline Gérard; Agnès Noël; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux
Archive | 2016
Céline Gérard; Anne Gallez; Silvia Blacher; Agnès Noël; Elisabete Silva; Anne Gompel; Françoise Lenfant; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux
Archive | 2016
Anne Gallez
Archive | 2016
Anne Gallez; Céline Gérard; Silvia Blacher; Agnès Noël; Jean-Michel Foidart; Christel Pequeux
Archive | 2015
Céline Gérard; Anne Gallez; Elisabete Silva; Anne Gompel; Françoise Lenfant; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux