Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Anne Gallez is active.

Publication


Featured researches published by Anne Gallez.


Journal of Pharmaceutical and Biomedical Analysis | 2017

Whole blood microsampling for the quantitation of estetrol without derivatization by liquid chromatography-tandem mass spectrometry

Gwenaël Nys; Anne Gallez; Miranda G.M. Kok; Gaël Cobraiville; Anne-Catherine Servais; Géraldine Piel; Christel Pequeux; Marianne Fillet

&NA; Quantitative bioanalysis and especially pharmacokinetic studies are challenging since only low volumes of biological material are available and low concentrations (ng/ml) are often expected. In this context, volumetric absorptive microsampling (VAMS) devices were developed to accurately collect 10 or 20 &mgr;l of whole blood from tested subjects. In this study, we present the development and validation of ultra‐high performance liquid chromatography coupled to tandem mass spectrometry method after VAMS sampling for the quantitation of estetrol (E4), a potentially new medicine for hormone replacement, contraception and osteoporosis therapies. Interestingly, a very simple sample preparation procedure was developed without any derivatization step. Even if lack of sensitivity is a common consideration when using negative ionization mode, we demonstrated in this work that an excellent sensitivity could be reached by carefully optimizing the nature and concentration of the mobile phase additive. After the optimization of every experimental parameter, the stability, selectivity, trueness, precision and accuracy of the final method were successfully demonstrated. In addition, the excellent performances of the method were confirmed by two independent proof‐of‐concept pharmacokinetic studies of E4 after VAMS collection in a murine model. Graphical abstract Figure. No caption available. HighlightsA UHPLC–MS/MS method is proposed for the analysis of estetrol without derivatization.NH4F as LC additive is used to reach needed sensitivity in negative mode.An innovative microsampling technique is used to collect 10 &mgr;l of blood from mice.The method was successfully validated for the quantitation of estetrol.A pharmacokinetic study is performed as proof‐of‐concept.


Endocrinology | 2016

Quantitative assessment of mouse mammary gland morphology using automated digital image processing and TEB detection.

Silvia Blacher; Céline Gérard; Anne Gallez; Jean-Michel Foidart; Agnès Noël; Christel Pequeux

The assessment of rodent mammary gland morphology is largely used to study the molecular mechanisms driving breast development and to analyze the impact of various endocrine disruptors with putative pathological implications. In this work, we propose a methodology relying on fully automated digital image analysis methods including image processing and quantification of the whole ductal tree and of the terminal end buds as well. It allows to accurately and objectively measure both growth parameters and fine morphological glandular structures. Mammary gland elongation was characterized by 2 parameters: the length and the epithelial area of the ductal tree. Ductal tree fine structures were characterized by: 1) branch end-point density, 2) branching density, and 3) branch length distribution. The proposed methodology was compared with quantification methods classically used in the literature. This procedure can be transposed to several software and thus largely used by scientists studying rodent mammary gland morphology.


Journal of Mammary Gland Biology and Neoplasia | 2017

Accurate Control of 17β-Estradiol Long-Term Release Increases Reliability and Reproducibility of Preclinical Animal Studies

Céline Gérard; Anne Gallez; Charline Dubois; Pierre Drion; Philippe Delahaut; Etienne Quertemont; Agnès Noël; Christel Pequeux

Estrogens are the subject of intensive researches aiming to elucidate their mechanism of action on the various tissues they target and especially on mammary gland and breast cancer. The use of ready-to-use slow releasing devices to administer steroids, especially estrogens, to small experimental animals remains the method of choice in terms of animal well-being and of safety for both the researcher and the animal. In this study, we evaluated and compared, in vitro and in vivo, the release kinetic of estradiol (E2) over sixty days from two different slow-releasing systems: the matrix pellet (MP) and the reservoir implant (RI). We compared the impact of these systems in three E2-sensitive mouse models : mammary gland development, human MCF7 adenocarcinoma xenograft and mouse melanoma progression. The real amount of E2 that is released from both types of devices could differ from manufacturer specifications due to inadequate release for MP and initial burst effect for RI. Compared to MP, the interindividual variability was reduced with RI thanks to a superior control of the E2 release. Depending on the dose-dependent sensitivity of the physiological or pathological readout studied, this could lead to an improvement of the statistical power of in vivo experiments and thus to a reduction of the required animal number. Altogether, our data draw attention on the importance to adequately select the slow-releasing device that is the most appropriated to a specific experiment to better fulfill the 3Rs rule (Replacement, Reduction, Refinement) related to animal welfare and protection.


Archive | 2017

Dose-dependent effect of Estetrol on Angiogenesis and Tumor Growth

Anne Gallez; Silvia Blacher; Françoise Lenfant; Jean-François Arnal; Myriam Polette; Philippe Birembaut; Agnès Noël; Jean-Michel Foidart; Christel Pequeux


Archive | 2017

Le microenvironement tumoral, un acteur essentiel de la différence liée au genre dans le développement des cancers pulmonaires

Charline Dubois; Silvia Blacher; Irina Primac; Melissa García Caballero; Anne Gallez; Natacha Rocks; Christel Pequeux; Didier Cataldo


Archive | 2017

ERα- and dose-dependent effect of estetrol on angiogenesis and tumor growth

Anne Gallez; Silvia Blacher; Céline Gérard; Agnès Noël; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux


Archive | 2016

Estetrol, a natural SERM exhibiting combined estrogenic and anti-estrogenic properties on mammary gland and breast cancer

Céline Gérard; Anne Gallez; Silvia Blacher; Agnès Noël; Elisabete Silva; Anne Gompel; Françoise Lenfant; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux


Archive | 2016

Etude des mécanismes d'activation des récepteurs aux oestrogènes par l'estétrol et/ou la progestérone dans le sein normal et tumoral

Anne Gallez


Archive | 2016

Impact of estetrol on breast cancer development, metastatic dissemination and angiogenesis

Anne Gallez; Céline Gérard; Silvia Blacher; Agnès Noël; Jean-Michel Foidart; Christel Pequeux


Archive | 2015

Estetrol : a new natural estrogen providing a safe therapeutic window for the treatment of menopause

Céline Gérard; Anne Gallez; Elisabete Silva; Anne Gompel; Françoise Lenfant; Jean-François Arnal; Jean-Michel Foidart; Christel Pequeux

Collaboration


Dive into the Anne Gallez's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Anne Gompel

Paris Descartes University

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge