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Dive into the research topics where Anne-Marie Lambert is active.

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Featured researches published by Anne-Marie Lambert.


The Plant Cell | 1994

Isolated Plant Nuclei Nucleate Microtubule Assembly: The Nuclear Surface in Higher Plants Has Centrosome-like Activity.

Virginie Stoppin; Marylin Vantard; Anne-Catherine Schmit; Anne-Marie Lambert

In most eukaryotic cells, microtubules (MTs) are assembled at identified nucleating sites, such as centrosomes or spindle pole bodies. Higher plant cells do not possess such centrosome-like structures. Thus, the fundamental issues of where and how the intracellular plant MTs are nucleated remain highly debatable. A large body of evidence indicates that plant MTs emerge from the nuclear periphery. In this study, we developed an in vitro assay in which isolated maize nuclei nucleate MT assembly at a tubulin concentration (14 [mu]M of neurotubulin) that is not efficient for spontaneous MT assembly. No MT-stabilizing agents, such as taxol or dimethyl sulfoxide, were used. Our model provides evidence that the nuclear surface functions as a MT-nucleating site in higher plant cells. A monoclonal antibody raised against a pericentriolar antigen immunostained the surface of isolated nuclei, and a 100-kD polypeptide in 4 M urea-treated nuclear extracts was detected.


The Plant Cell | 1990

Microinjected fluorescent phalloidin in vivo reveals the F-actin dynamics and assembly in higher plant mitotic cells.

Anne-Catherine Schmit; Anne-Marie Lambert

Endosperm mitotic cells microinjected with fluorescent phalloidin enabled us to follow the in vivo dynamics of the F-actin cytoskeleton. The fluorescent probe immediately bound to plant microfilaments. First, we investigated the active rearrangement of F-actin during chromosome migration, which appeared to be slowed down in the presence of phalloidin. These findings were compared with the actin patterns observed in mitotic cells fixed at different stages. Our second aim was to determine the origin of the actin filaments that appear at the equator during anaphase-telophase transition. It is not clear whether this F-actin is newly assembled at the end of mitosis and could control plant cytokinesis or whether it corresponds to a passive redistribution of broken polymers in response to microtubule dynamics. We microinjected the same cells twice, first in metaphase with rhodamine-phalloidin and then in late anaphase with fluorescein isothiocyanate-phalloidin. This technique enabled us to visualize two F-actin populations that are not co-localized, suggesting that actin is newly assembled during cell plate development. These in vivo data shed new light on the role of actin in plant mitosis and cytokinesis.


Chromosoma | 1980

The role of chromosomes in anaphase trigger and nuclear envelope activity in spindle formation

Anne-Marie Lambert

In order to analyse the role of the nuclear envelope (NE) and chromosomes themselves in spindle dynamics, two events were selected: prophase clear-zone formation and anaphase. Data in vivo were precisely correlated with fine structure. Spore mother cells of the moss Mnium hornum and endosperm of Haemanthus albiflos and H. katherinae were used as material.During Pachytene, in Mnium, which occurs between +5° to −5° C in natural environmental conditions, microtubules (Mts) are formed and regularly distributed on the surface of the nucleus, particularly in regions facing the poles. They are anchored to the NE. During NE breakage, followed by rapid Mt assembly, fragments of the envelope are entirely surrounded with evenly spaced Mts and often migrate to the poles. In prophase of Haemanthus, at 22° C, Mts are irregularly arranged around the nucleus and direct connections with NE are evident. The data presented suggest that NE is a Mt nucleating site during initial stages of spindle development. Mt distribution is correlated to temperature conditions of Mt assembly. — Effects of recovery at 22° C after cold shock (+3° C) were studied: Mt nucleation is experimentally speeded up and Mts grow with random orientation, never found in controls, at all stages of mitosis. — Start of anaphase and course of kinetochore splitting was followed in vivo (time lapse) during normal division and under inhibition of Mt assembly in cells treated with colchicine and vinblastine. Autonomous chromatid repulsion and kinetochore separation up to 3.5 to 4 μm take place without traces of kinetochore Mts. This process is general but is obscured in normal divisions by chromosome alignment at equatorial plane. It is concluded that spindle independent kinetochore splitting is a chromosomal trigger for poleward migration. It preceeds anaphase movements and occurs at a precise stage of the chromosome cycle.


Microscopy Research and Technique | 2000

Higher Plant Cells: Gamma-Tubulin and Microtubule Nucleation in the Absence of Centrosomes

Jean Canaday; Virginie Stoppin-Mellet; Jérôme Mutterer; Anne-Marie Lambert; Anne-Catherine Schmit

The assembly of the higher plant cytoskeleton poses several fundamental questions. Since different microtubule arrays are successively assembled during the cell cycle in the absence of centrosomes, we can ask how these arrays are assembled and spatially organized. Two hypotheses are under debate. Either multiple nucleation sites are responsible for the assembly and organization of microtubule arrays or microtubule nucleation takes place at one site, the nuclear surface. In the latter case, microtubule nucleation and organization would be two distinct but coregulated processes. During recent years, novel approaches have provided entirely new insights to understand the assembly and dynamics of the plant cytoskeleton. In the present review, we summarize advances made in microscopy and in molecular biology which lead to novel hypotheses and open up new fields of investigation. From the results obtained, it is clear that the higher plant cell is a powerful model system to investigate cytoskeletal organization in acentrosomal eukaryotic cells. Microsc. Res. Tech. 49:487–495, 2000.


Biology of the Cell | 1988

Plant actin filament and microtubule interactions during anaphase‐telophase transition: effects of antagonist drugs

Anne-Catherine Schmit; Anne-Marie Lambert

F‐actin and microtubule co‐distribution and interaction were studied during anaphase‐telophase. Rapid and drastic changes in the cytoskeleton during these particular stages were studied in isolated plant endosperm cells of the blood lily. These wall‐free cells can be considered as natural dividing protoplasts. As identified previously, an F‐actin cytoskeletal network characterized the plant cortex and formed an elastic cage around the spindle, remaining throughout interphase, mitosis and cytokinesis. Actin was specifically labeled by fluorescent phalloidin and/or monoclonal antibodies. Gold‐labeled secondary antibodies were used for ultrastructural observations and silver‐enhancement was applied for video‐enhanced microscopy. Microtubule and microfilament dynamics and interaction were studied using drug antagonists to actin (cytochalasins B, D) and to tubulin (colchicine). This permitted precise correlations to be made between chromosome movement inhibition and alteration in the actin/tubulin cytoskeleton. During anaphase chromosome migration, the cortical actin network was stretched along the microtubular spindle, while it remained homogeneous when anaphase was inhibited by colchicine. Cytochalasins did not inhibit chromosome movement but altered actin distribution. A new population of actin filaments appeared at the equator in late anaphase before the microtubular phragmoplast was formed and contributed to cell plate formation. Our conclusion is that F‐actin‐microtubule interaction may contribute to the regulatory mechanism of plant cytokinesis.


European Journal of Cell Biology | 1998

The growing cell plate of higher plants is a site of both actin assembly and vinculin-like antigen recruitment

Marie-Christine Endlé; Virginie Stoppin; Anne-Marie Lambert; Anne-Catherine Schmit

Compelling evidence supports the idea that actin filaments play an active role in the cytokinetic process of higher plant cells. However, the mechanisms that control the growth of the cell plate and its stabilization remain so far unknown. We show that a novel population of short actin filaments continuously assembles in the phragmoplast at the growing cell plate. Microinjection of rhodamine-phalloidin during these final stages of telophase revealed the dynamic assembly and organization of these actin filaments during vesicle fusion. Comparable data were obtained in endosperm syncytia during the development of the cell plate between non sister nuclei, i.e. independently of the formation of the mitotic phragmoplast. Concomitantly, plant polypeptides sharing epitopes with human vinculin are revealed within the forming cell plate, suggesting their recruitment during cytokinesis-associated actin assembly. These vinculin-like antigens may participate in membrane/F-actin anchorage protein complexes. Our data, in addition to the identification of plant integrin homologues reported by several authors, suggest the existence of a cell wall/extracellular matrix/plasma membrane/actin cytoskeleton continuum. Such an architecture may control cell-cell interactions during cell plate formation and may contribute to the establishment of polarity in higher plants.


Chromosoma | 1994

Cell cycle dependent distribution of a centrosomal antigen at the perinuclear MTOC or at the kinetochores of higher plant cells

Anne-Catherine Schmit; Virginie Stoppin; Véronique Chevrier; Didier Job; Anne-Marie Lambert

Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains entirely unknown. Using a monoclonal antibody (mAb 6C6) raised against isolated calf thymus centrosomes and showing apparent reaction with the plant nuclear surface, we followed the targeted antigen distribution during mitosis and meiosis of higher plants. Immunoblot analysis of protein fractions from Allium root meristematic cell extracts probed with mAb 6C6 reveals a polypeptide of an apparent Mr of 78000. In calf centrosome extracts, a polypeptide of comparable molecular mass is found in addition to a major antigen of Mr 180000 after mAb 6C6 immunoblotting. During mitotic initiation, the plant antigen is prominent on the periphery of the prophase nucleus. When the nuclear envelope breaks down, the antigen suddenly becomes associated with the centromere-kinetochores until late anaphase. In telophase, when the nuclear envelope is being reconstructed, it is no longer detected at the kinetochores but is solely associated again with the nuclear surface. This antigen displays a unique spatial and temporal distribution, which may reflect the pathway of plant protein(s) between the nuclear surface and the kinetochores under cell cycle control. So far, such processes have not been described in higher plant cells. These observations shed light on the putative activity of the plant kinetochore as a protein transporter. They also suggest that a plant centrosome-like antigen may have different cytoskeletal related functions depending on cell cycle regulated changes in its subcellular distribution.


Chromosoma | 1996

The perinuclear microtubule-organizing center and the synaptonemal complex of higher plants share a common antigen: its putative transfer and role in meiotic chromosomal ordering.

Anne-Catherine Schmit; Marie-Christine Endlé; Anne-Marie Lambert

Recognition of homologous chromosomes during meiotic prophase is associated in most cases with the formation of the synaptonemal complex along the length of the chromosome. Telomeres, located at the nuclear periphery, are preferential initiation sites for the assembly of the synaptonemal complex. In most eukaryotic cells, telomeres cluster in a restricted area, leading to the “bouquet” configuration in leptotene-zygotene, while this typical organization progressively disappears in late zygotene-pachytene. We wondered whether such striking changes in the intranuclear ordering and pairing of meiotic chromosomes during the progression of prophase I could be correlated with activity of the centrosome and/or microtubule-organizing center (MTOC). Plant cells may be used as a model of special interest for this study as the whole nuclear surface acts as an MTOC, unlike other cell types where MTOCs are restricted to centrosomes or spindle pole bodies. Using a monoclonal antibody (mAb 6C6) raised against isolated calf centrosomes we found that the 6C6 antigen is present over the entire surface of the plant meiotic nucleus, in early prophase I, before chromosomal pairing. At zygotene, short fragments of chromosomes become stained near the nuclear envelope and within the nucleus. At pachytene, after complete synapsis, the labeling specifically concentrates within the synaptonemal complexes, although the nuclear surface is no longer reactive. Ultrastructural localization using immunogold labeling indicates that the 6C6 antigen is colocalized with the synaptonemal complex structures. Later in metaphase I, the antigen is found at the kinetochores. Our data favor the idea that the 6C6 antigen may function as a particular “chromosomal passenger-like’ protein. These observations shed new light on the molecular organization of the plant synaptonemal complex and on the redistribution of cytoskeleton-related antigens during initiation of meiosis. They suggest that antigens of MTOCs are relocated to chromosomes during the synapsis process starting at telomeres and contribute to the spatial arrangement of meiotic chromosomes. Such cytoskeleton-related antigens may acquire different functions depending on their localization, which is cell-cycle regulated.


European Journal of Cell Biology | 1999

Characterization of microsome-associated tobacco BY-2 centrins

Virginie Stoppin-Mellet; Jean Canaday; Anne-Marie Lambert

Centrin - higher plants - MTOCs - microtubules nucleation In most eukaryotic cells, the Ca(2+)-binding protein centrin is associated with structured microtubule-organizing centers (MTOCs) such as centrosomes. In these cells, centrin either forms centrosome-associated contractile fibers, or is involved in centrosome biogenesis. Our aim was to investigate the functions of centrin in higher plant cells which do not contain centrosome-like MTOCs. We have cloned two tobacco BY-2 centrin cDNAs and we show that higher plant centrins define a phylogenetic group of proteins distinct from centrosome-associated centrins. In addition, tobacco centrins were found primarily associated with microsomes and did not colocalize with gamma-tubulin, a known MTOC marker. While the overall level of centrin did not vary during the cell cycle, centrin was prominently detected at the cell plate during telophase. Our results suggest that in tobacco, the major portion of centrin is not MTOC-associated and could be involved in the formation of the cell plate during cytokinesis.


Plant Molecular Biology | 2002

A novel pollen-specific α-tubulin in sunflower: structure and characterization

Jean-Luc Evrard; Isabelle Nguyen; Marc Bergdoll; Jérôme Mutterer; André Steinmetz; Anne-Marie Lambert

We describe here a new α-tubulin isoform from sunflower we named απ-tubulin. απ-tubulin is the most divergent higher-plant α-tubulin described so far, having an unusual deletion in the H1/B2 loop and a glutamine-rich C-terminus. We constructed a three-dimensional model and discuss its implications. Using specific antibodies, we show that απ-tubulin expression is restricted to the male gametophyte. απ-tubulin mRNA represents 90% of α-tubulin mRNA and a small percentage of total pollen mRNA. Among the plants tested, απ-tubulin was only detected in sunflower and in Cosmos. Since both plants are Asteraceae, we propose that απ-tubulin is specific to this family. Our results suggest that απ-tubulin can inhibit tubulin assembly in pollen. This hypothesis is reinforced by the fact that απ-tubulin is found in a complex with β-tubulin in mature sunflower pollen.

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Anne-Catherine Schmit

Centre national de la recherche scientifique

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Marylin Vantard

Centre national de la recherche scientifique

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Christine Peter

Centre national de la recherche scientifique

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Jean Canaday

Centre national de la recherche scientifique

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Jérôme Mutterer

Centre national de la recherche scientifique

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Paul Schellenbaum

Centre national de la recherche scientifique

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Virginie Stoppin

Centre national de la recherche scientifique

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Jean-Luc Evrard

Centre national de la recherche scientifique

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Marie-Christine Endlé

Centre national de la recherche scientifique

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