Annette Pfennig
Humboldt University of Berlin
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Publication
Featured researches published by Annette Pfennig.
IEEE Engineering in Medicine and Biology Magazine | 2003
Torsten Müller; Annette Pfennig; Petra Klein; Gabriele Gradl; Ma Gnus Jäger; Thomas Schnelle
In this article, a microfluidic system was developed for sorting and high-content analysis of single cells using dielectrophoresis. It makes high-resolution imaging of suspended cells feasible immediately before depositing them into a microtiter plate. The microfluidic devices contain three-dimensional arrangements of radio-frequency driven electrodes and are used to gently manipulate suspended cells in flow. Viability of sorted and deposited cells is comparable to a control group. The influence of RF electric fields is studied using (a) calcium flux measurements and membrane potential dependent fluorescence dye on suspended human cells and (b) cultivation of yeast trapped for several days in dielectric field cages driven at field strengths up to 100 kV/m and 7 MHz.
Archive | 2004
Torsten Müller; Stefan Hummel; Annette Pfennig; Gabriele Gradl; Axel Bonsen; Rüdiger Meyer
Archive | 2003
Torsten Müller; Annette Pfennig; Stephen Graham Shirley; Stefan Hummel; Martin Reichel
Archive | 2005
Thomas Schnelle; Annette Pfennig; Torsten Müller
Archive | 2004
Torsten Müller; Annette Pfennig; Thomas Schnelle
Archive | 2005
Thomas Schnelle; Torsten Müller; Annette Pfennig
Archive | 2004
Torsten Müller; Stefan Hummel; Annette Pfennig
Archive | 2004
Torsten Müller; Stefan Hummel; Annette Pfennig
Archive | 2004
Torsten Müller; Stefan Hummel; Annette Pfennig; Gabriele Gradl; Axel Bonsen; Thomas Schnelle; Rüdiger Meyer
Nature Methods | 2005
Philipp Koch; Andrea Biegler; Annette Pfennig; Torsten Müller; Anne Klein-Vehne; Stefanie Terstegge; Joseph Itskovitz-Eldor; Oliver Brüstle