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Dive into the research topics where Annette Pfennig is active.

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Featured researches published by Annette Pfennig.


IEEE Engineering in Medicine and Biology Magazine | 2003

The potential of dielectrophoresis for single-cell experiments

Torsten Müller; Annette Pfennig; Petra Klein; Gabriele Gradl; Ma Gnus Jäger; Thomas Schnelle

In this article, a microfluidic system was developed for sorting and high-content analysis of single cells using dielectrophoresis. It makes high-resolution imaging of suspended cells feasible immediately before depositing them into a microtiter plate. The microfluidic devices contain three-dimensional arrangements of radio-frequency driven electrodes and are used to gently manipulate suspended cells in flow. Viability of sorted and deposited cells is comparable to a control group. The influence of RF electric fields is studied using (a) calcium flux measurements and membrane potential dependent fluorescence dye on suspended human cells and (b) cultivation of yeast trapped for several days in dielectric field cages driven at field strengths up to 100 kV/m and 7 MHz.


Archive | 2004

Multiparametric cell identification and sorting method and associated device

Torsten Müller; Stefan Hummel; Annette Pfennig; Gabriele Gradl; Axel Bonsen; Rüdiger Meyer


Archive | 2003

Device and method for coupling lines to fluidic microsystems

Torsten Müller; Annette Pfennig; Stephen Graham Shirley; Stefan Hummel; Martin Reichel


Archive | 2005

Microfluidic system comprising an electrode arrangement and associated control method

Thomas Schnelle; Annette Pfennig; Torsten Müller


Archive | 2004

Microfluid cell sorting system with electrodes delivers a flow of particles in suspension to first and second arrays of sorting electrodes

Torsten Müller; Annette Pfennig; Thomas Schnelle


Archive | 2005

Micro-fluidic system comprising an expanded channel

Thomas Schnelle; Torsten Müller; Annette Pfennig


Archive | 2004

Docking device for a fluidic microsystem

Torsten Müller; Stefan Hummel; Annette Pfennig


Archive | 2004

Sample depositing device for a cell sorter

Torsten Müller; Stefan Hummel; Annette Pfennig


Archive | 2004

Multiparametric detection in a fluidic microsystem

Torsten Müller; Stefan Hummel; Annette Pfennig; Gabriele Gradl; Axel Bonsen; Thomas Schnelle; Rüdiger Meyer


Nature Methods | 2005

Automated generation of human stem cell clones by Image-Activated Cell Selection (IACS|[trade]|)

Philipp Koch; Andrea Biegler; Annette Pfennig; Torsten Müller; Anne Klein-Vehne; Stefanie Terstegge; Joseph Itskovitz-Eldor; Oliver Brüstle

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Torsten Müller

Humboldt University of Berlin

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Thomas Schnelle

Humboldt University of Berlin

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Gabriele Gradl

Humboldt University of Berlin

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Petra Klein

Humboldt University of Berlin

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Ma Gnus Jäger

Humboldt University of Berlin

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