Annica Pilgård

Mode of action of brown rot decay resistance in modified wood: A review

Abstract Chemically or physically modified wood materials have enhanced resistance to wood decay fungi. In contrast to treatments with traditional wood preservatives, where the resistance is caused mainly by the toxicity of the chemicals added, little is known about the mode of action of nontoxic wood modification methods. This study reviews established theories related to resistance in acetylated, furfurylated, dimethylol dihydroxyethyleneurea-treated, and thermally modified wood. The main conclusion is that only one theory provides a consistent explanation for the initial inhibition of brown rot degradation in modified wood, that is, moisture exclusion via the reduction of cell wall voids. Other proposed mechanisms, such as enzyme nonrecognition, micropore blocking, and reducing the number of free hydroxyl groups, may reduce the degradation rate when cell wall water uptake is no longer impeded.

Toxic hazard and chemical analysis of leachates from furfurylated wood

The furfurylation process is an extensively investigated wood modification process. Furfuryl alcohol molecules penetrate into the wood cell wall and polymerize in situ. This results in a permanent swelling of the wood cell walls. It is unclear whether or not chemical bonds exist between the furfuryl alcohol polymer and the wood. In the present study, five different wood species were used, both hardwoods and softwoods. They were treated with three different furfurylation procedures and leached according to three different leaching methods. The present study shows that, in general, the leachates from furfurylated wood have low toxicity. It also shows that the choice of leaching method is decisive for the outcome of the toxicity results. Earlier studies have shown that leachates from wood treated with furfuryl alcohol prepolymers have higher toxicity to Vibrio fischeri than leachates from wood treated with furfuryl alcohol monomers. This is probably attributable to differences in leaching of chemical compounds. The present study shows that this difference in the toxicity most likely cannot be attributed to maleic acid, furan, furfural, furfuryl alcohol, or 2-furoic acid. However, the difference might be caused by the two substances 5-hydroxymethylfurfural and 2,5-furandimethanol. The present study found no difference in the amount of leached furfuryl alcohol between leachates from furfurylated softwood and furfurylated hardwood species. Earlier studies have indicated differences in grafting of furfuryl alcohol to lignin. However, nothing was found in the present study that could support this. The leachates of furfurylated wood still need to be

qPCR as a tool to study basidiomycete colonization in wooden field stakes

Abstract Molecular methods are emerging also as useful tools for wood protection studies. The aim of the present study was to evaluate quantitative real-time polymerase chain reaction (qPCR) as a tool for investigating details of the colonization pattern of basidiomycete decay fungi in wood samples after 6 years of soil exposure. Samples of Pinus sylvestris L. (heartwood without treatment), furfurylated P. sylvestris sapwood and Cu-HDO treated P. sylvestris sapwood was in focus. The qPCR method based on basidiomycete DNA content in the wood had the highest sensitivity, while the ergosterol assay was more sensitive than the chitin assay. Visual rating was compared with laboratory analyses and was found to be correlating well with qPCR. This study demonstrates that qPCR in combination with microscopy provides relevant data about basidiomycete colonization in wooden material.

Quantification of fungal colonization in modified wood: quantitative real-time PCR as a tool for studies on Trametes versicolor.

Abstract Traditional wood preservatives based on biocides are effective against wood-deteriorating organisms because of their toxicity. By contrast, modified woods are non-toxic by definition. To investigate the efficiency of various wood modifications, quantitative real-time polymerase chain reaction (qPCR) was used to profile the DNA amounts of the white-rot fungus Trametes versicolor (L.) [Lloyd strain CTB 863 A] during an 8-week-long growth period in treated Pinus sylvestris (L.) sapwood. The studied wood was modified by acetylation, furfurylation, and thermal treatment. The traditional wood preservatives bis-(N-cyclohexyldiazeniumdioxy)-copper (Cu-HDO) and chromated copper arsenate (CCA) were used as references, whereas untreated P. sylvestris (L.) sapwood served as a control. The maximum levels of fungal DNA in native wood occurred at the end of the experiment. For all wood treatments, the maximum fungal DNA level was recorded after an incubation period of 2 weeks, followed by a decline until the end of the trial. For the preservative-treated woods, Cu-HDO showed the lowest level of fungal DNA throughout the experiment, indicating that exploratory hyphal growth is limited owing to the phytotoxicity of the treatment. The other treatments did not inhibit the exploratory hyphal growth phase. We conclude that qPCR studies of hyphal growth patterns within wood should provide a powerful tool for evaluating and further optimizing new wood protection systems.

The role of chemical transport in the brown-rot decay resistance of modified wood

Chemical modification of wood increases decay resistance but the exact mechanisms remain poorly understood. Recently, Ringman and coauthors examined established theories addressing why modified wood has increased decay resistance and concluded that the most probable cause of inhibition and/or delay of initiation of brown-rot decay is lowering the equilibrium moisture content. In another recent study, Jakes and coauthors examined moisture-induced wood damage mechanisms, including decay and fastener corrosion, and observed that these mechanisms require chemical transport through wood cell walls. They proposed that chemical transport within wood cell walls is controlled by a moisture-induced glass transition in interconnected networks of hemicelluloses and amorphous cellulose. This paper shows how these models jointly suggest mechanisms by which wood modifications can inhibit brown-rot. Alternative mechanisms are also discussed. These models can be used to understand and further improve the performance of wood modification systems.

The activity of rot fungi (Postia placenta) during drying and rewetting cycles measured by isothermal calorimetry

Rot fungi are a major problem in the construction sector, and method to study under which moisture and temperature coefficients they grow are therefore of significant interest. Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents (MCs). The results clearly show the heat production rate (a measure of respiration rate and fungal activity) is moisture‐dependent. For most cases, less heat was produced when the MC was decreased, and more heat was produced when the MC was increased. It was also found that when the MC increased after a dry period, the increase in activity was significantly delayed. However, if the moisture state was then kept constant at a high level, the activity slowly increased, showing that the fungi need time to recover back to the original activity level after drying. Isothermal calorimetry is a measurement technique well suited for the study of the activity of wood‐decaying fungi as a function of temperature and moisture content.

New insight regarding mode of action of brown rot decay of modified wood based on DNA and gene expression studies: a review

Abstract Modified wood shows resistance against wood deteriorating fungi, but the mechanisms are still not fully understood. The aim of this paper was to summarise the molecular studies performed on modified wood with regard to brown rot decay fungi. The DNA data showed that fungi are present inside the laboratory wood test samples already after two weeks of inoculation. Generally the fungal DNA content reflects mass loss and wood moisture content. The oxidative gene expression seems to be higher in modified wood than in untreated wood and it tend to increase during incubation. Based on the gene expression data we suggest that the hypothesis of lack of substrate recognition by the fungus should be rejected. In the reviewed studies, total wood moisture content in the samples was generally not low enough to inhibit fungal colonisation. Hence, moisture distribution within the wood should be studied more closely.

Postia placenta decay of acetic anhydride modified wood – Effect of leaching

Abstract It is well established that acetylation of wood by the use of acetic anhydride is able to impart a significant degree of decay resistance. The aim of this work was to study how a standardized leaching procedure with water (EN 84) affected the degradation of acetic anhydride modified samples by the brown rot fungi Postia placenta compared to no leaching prior to incubation. Three different levels (low, medium, and high) of acetic anhydride modified Southern yellow pine (SYP; Pinus spp.) were tested. The samples were harvested after 4 and 28 weeks. We compared changes in mass loss, wood moisture content, fungal DNA, and gene expression from five genes. If leaching changes the acetylated samples and makes them more susceptible for fungal deterioration, the expected effect would be higher levels of these parameters. Generally, leaching resulted in few differences between leached and nonleached samples at low levels of acetylation, while no changes were found for the highest acetylation level. No differences were found in gene expressions after 28 weeks. The possible protection of acetylated wood against oxidative fungal degradation is suggested to be interpreted in combination with the lowered wood moisture content.

Combined evaluation of durability and ecotoxicity: A case study on furfurylated wood

Abstract Modified wood is commercially available and merchandized as a new, environmentally friendly and durable wood species. However, there are no standards focusing on the evaluation of modified wood. Combining resistance against fungal decay and good ecotoxicological properties may be a start. In this study softwood and hardwood species were furfurylated using different treatment processes and treating solutions. The durability was determined by exposing the treated wood to a range of Basidiomycetes and the ecotoxicity was studied on two aquatic organisms. It was the purpose to come to a strategy and how to unite efficacy and ecotoxicity, since this is important in product development. The results show that the selection of fungus used for mass loss determination and the choice of ecotoxicity method is decisive, confirming that a combination of methods is valuable. A tiered approach to find the optimal treatment seems the best option. First, adequate protection against wood-rotting fungi should be attained, followed by ecotoxicity evaluation of the wood leachates. If necessary, the optimization process should be repeated until both durability and ecotoxicity are within satisfactory limits. This process could be extended with other evaluation criteria, e.g. dimensional stability of the modified wood or a risk analysis of its leachate.

In vitro oxidative and enzymatic degradation of modified wood

Abstract Fungal cellulases have been shown to be less efficient in modified wood than in untreated wood (; ). However, showed that cellulase efficacy is partly restored in 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU) treated wood by pre-treatment with Fenton’s reagent, simulating the oxidative degradation phase in initial brown rot decay. In this study, we examined whether Fenton derived hydroxyl radicals (·OH) and cellulases are able to cleave polysaccharides in furfurylated and acetylated wood and to what extent enzyme efficacy is increased by oxidative pre-treatment of these materials. The results show that fungal cellulases were able to degrade acetylated wood and that the cellulase efficacy was increased by oxidative pre-treatment by 20%, which is half of the increase in untreated wood. Furthermore, the results indicate that poly(furfuryl alcohol) is degraded by Fenton derived ·OH. This indicates a possible route for the eventual degradation of modified wood.