Annie Couteau
Institut national de la recherche agronomique
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Publication
Featured researches published by Annie Couteau.
Journal of Microbiological Methods | 2002
Stéphane Poussier; Jean-Jacques Cheron; Annie Couteau; Jacques Luisetti
Several procedures were compared for reliable PCR detection of Ralstonia solanacearum in common substrates (plant, seed, water and soil). In order to prevent the inhibition of PCR by substances contained in crude extracts, numerous DNA extraction procedures as well as additives to buffers or PCR mixtures were checked. Our results showed that the efficiency of these methods or compounds depended greatly upon the nature of the sample. Consequently, preparation of samples prior to PCR depended upon sample origin. Simple methods such as a combined PVPP/BSA treatment or the association of filtration and centrifugation for detecting the bacterium in plant or water samples were very powerful. DNA capture also efficiently overcame PCR inhibition problems and ensured the detection of R. solanacearum in environmental samples. However, the commercial DNA extraction QIAamp kit appeared to be the most effective tool to guarantee the accurate PCR detection of the pathogen whatever the origin of the sample; this was particularly true for soil samples where the commonly used methods for the detection of R. solanacearum were inefficient. This study demonstrates that using an appropriate procedure, PCR is a useful and powerful tool for detecting low levels of R. solanacearum populations in their natural habitats.
Journal of Applied Microbiology | 1998
Olivier Pruvost; Annie Couteau; Xavier Perrier; Jacques Luisetti
Carbohydrate utilization profiles by means of the API (Appareils et Procédés d’Identification) system and sensitivity to antibiotics and heavy metal salts of 68 Xanthomonas sp. mangiferaeindicae strains isolated in nine countries from mango (Mangifera indica L.) and other genera of the Anacardiaceae were examined to assess the variability of the taxon. The strains could be separated into 10 groups according to Ward clustering. Apigmented strains isolated from the pepper tree [syn. Brazilian pepper] (Schinus terebenthifolius Raddi) could not be clearly differentiated from most apigmented strains isolated from mango. Yellow‐pigmented strains isolated from mango in Brazil and Reunion Island, apigmented strains isolated from mango in Brazil and from ambarella in the French West Indies, clustered in distinct groups. The results are consistent with those of other studies, based on isozyme analysis of esterase, phosphoglucomutase and superoxide dismutase, and hrp‐RFLP analysis; they indicate the need for a comprehensive taxonomic evaluation of xanthomonads associated with Anacardiaceae.
Fruits | 1990
Olivier Pruvost; Annie Couteau; Jacques Luisetti
Fruits | 1991
Christian Vernière; M. Devaux; Olivier Pruvost; Annie Couteau; Jacques Luisetti
Archive | 2001
Olivier Pruvost; Lionel Gagnevin; Véronique Glories; Annie Couteau; K. Mete; B. Flahaut
Fruits | 1989
Olivier Pruvost; Annie Couteau; Jacques Luisetti
Archive | 2005
Laurence Humeau; Philippe Roumagnac; Isabelle Soustrade; Annie Couteau; Frédéric Chiroleu; Gareth Hughes; Olivier Pruvost
Archive | 2001
Olivier Pruvost; Lionel Gagnevin; Véronique Glories; Annie Couteau; K. Mete
Proceedings of the Sixth International Symposium on Mango, Pattaya City (Thailand), 6-9 April, 1999 | 2000
Olivier Pruvost; Lionel Gagnevin; Véronique Glories; Annie Couteau; A. Some; Régine Samson
Archive | 2000
Lionel Gagnevin; Véronique Glories; Annie Couteau; K. Mete; L. Gout; Olivier Pruvost
Collaboration
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Centre de coopération internationale en recherche agronomique pour le développement
View shared research outputsCentre de coopération internationale en recherche agronomique pour le développement
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