Antoinette Pirie

A Light-catalysed Reaction in the Aqueous Humour of the Eye

Kon and Watson1 found that ascorbic acid in milk was destroyed by light and Hopkins2 showed that this destruction was caused by the photocatalytic oxidation of ascorbic acid by riboflavin. Oxygen was necessary and peroxide was formed. In connexion with an examination of glutathione peroxidase of lens I became interested in the ascorbic acid of the aqueous humour as a possible source of hydrogen peroxide. In many mammals, including man, the level of ascorbic acid in the aqueous humour is about 1.0 mM, which is far higher than the level in serum3. Light freely penetrates through the aqueous humour whenever the eye is open, and Philpot and Pirie4 found traces of riboflavin and/or flavinadenine dinucleotide in the aqueous humour of cattle eyes. It seemed possible, therefore, that a photocatalysed oxidation of ascorbic acid might take place in the eye in vivo, as it does in milk in vitro.

A Complete Laboratory

Biochemistry of the EyeEdited by Clive N. Graymore. Pp. xiii + 783. (Academic: London and New York, June 1970.) 240s;

GLUTATHIONE PEROXIDASE IN LENS AND A SOURCE OF HYDROGEN PEROXIDE IN AQUEOUS HUMOUR

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