Antonella Baron
University of Verona
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Publication
Featured researches published by Antonella Baron.
Oncogene | 2002
Tatjana Crnogorac-Jurcevic; Evangelos Efthimiou; Torsten O. Nielsen; Julie Loader; Benoit Terris; Gordon Stamp; Antonella Baron; Aldo Scarpa; Nicholas R. Lemoine
Pancreatic ductal adenocarcinoma is characterized by a paucity of neoplastic cells embedded in a densely desmoplastic stroma. Therefore, laser capture microdissection was performed to obtain homogeneous populations of normal and neoplastic ductal cells. These were subjected to a comparative study of gene expression utilizing human cDNA arrays. A variety of dysregulated genes were identified, comprising cell cycle and growth regulators, invasion regulators, signalling and developmental molecules. In addition to genes already found to be overexpressed in pancreatic cancer, such as TIMP1, MMP7, CD59, rhoC and NDKA, we present evidence to implicate genes which have not previously been reported in this tumour type. These include the overexpressed genes ABL2, Notch4 and SOD1, as well as XRCC1, a DNA repair gene whose transcript was found downregulated. Quantitative real-time RT–PCR (QRT–PCR) was employed to confirm differential expression of ABL2, Notch4 and SOD1 and immunohistochemical analysis was used to verify decreased protein expression of XRCC1 using a custom-built pancreatic tissue array. Combining microarray-derived gene expression profiles of pure pancreatic cell populations, QRT–PCR and pancreas-specific tissue arrays therefore proved to be highly informative in elucidating the molecular pathology of this highly malignant tumour type.
Oncogene | 2001
Tatjana Crnogorac-Jurcevic; Evangelis Efthimiou; Paola Capelli; Ekaterina Blaveri; Antonella Baron; Benoit Terris; Melanie Jones; Kerry Tyson; Claudio Bassi; Aldo Scarpa; Nicholas R. Lemoine
Gene expression studies were undertaken in normal pancreas and pancreatic adenocarcinomas to determine new candidate genes that can potentially be used as markers of the disease. The characteristic desmoplastic stromal reaction of pancreatic adenocarcinoma greatly hampers expression studies in this tumour type, and usually necessitates time-consuming tissue microdissection for enrichment of the tumour cell population. We show that fine needle aspiration of cancer provides a fast and efficient way of obtaining samples highly enriched in tumour cells with sufficient yields of RNA. Using Atlas cancer cDNA arrays with 588 cancer-related genes, we describe gene expression profiles of normal pancreas, bulk pancreatic tumour tissues and pancreatic tumour aspirates containing more than 95% tumour cells. Analysis of bulk tissue specimens revealed differentially expressed genes belonging predominantly to the stromal component of the tumour. This contrasted with the results obtained from tumour-cell enriched samples. Several genes already described in pancreatic cancer (caspase 8, TIMP1, CD9, IL-13) were also differentially expressed in our study. Furthermore, we found dysregulated expression of genes not previously associated with pancreatic adenocarcinoma, such as Rac 1, GLG1, NEDD5, RPL-13a, RPS9 and members of the Wnt5A gene family. In summary, we present a panel of genes newly identified in the pathogenesis of pancreatic adenocarcinoma and demonstrate that fine needle aspirates of the tumour mass are a convenient source of material for gene expression studies in tumours accompanied by desmoplastic reactions.
Virchows Archiv | 2002
Aldo Scarpa; Simonetta Orlandini; Patrick S. Moore; Nicholas R. Lemoine; Stefania Beghelli; Antonella Baron; Massimo Falconi; Giuseppe Zamboni
Abstract.DPC4/Smad4 is inactivated in about 50% of pancreatic ductal cancers. It has been recently reported that this gene is also inactivated in neoplasms arising from pancreatic islet cells, a phenomenon suggested to be related to similar progressions of malignancy found in common ductal cancers. To evaluate this possibility, we analysed 20 metastases of pancreatic endocrine carcinomas and their corresponding primary lesion for inactivation of DPC4 using immunohistochemical staining. In fact, immunohistochemical labelling has been shown to correlate with DPC4 gene status with high sensitivity and specificity. The cancers included 18 nonfunctioning tumours, one gastrinoma and one VIPoma all with liver, nodal and/or adrenal metastases. Seventeen were well-differentiated and three poorly differentiated endocrine carcinomas. Dpc4 expression was absent in only one primary well-differentiated endocrine cancer and its liver metastasis, while all the remaining 19 primary tumours and their metastases stained positive for the protein. All positively staining cases showed diffuse cytoplasmic and nuclear staining in virtually all neoplastic cells. Our data suggest that DPC4 is only rarely involved in pancreatic endocrine tumourigenesis and give further weight to the hypothesis that tumours arising from pancreatic exocrine and endocrine epithelia are genetically distinct.
Cancer Research | 2001
Gildas Rigaud; Edoardo Missiaglia; Patrick S. Moore; Giuseppe Zamboni; Massimo Falconi; Giorgio Talamini; Anna Pesci; Antonella Baron; Daniele Lissandrini; Guido Rindi; Piergiovanni Grigolato; Paolo Pederzoli; Aldo Scarpa
Cancer Research | 1999
Claudio Sorio; Antonella Baron; Simonetta Orlandini; Giuseppe Zamboni; Paolo Pederzoli; Kay Huebner; Aldo Scarpa
Blood | 2003
Lucia De Franceschi; Antonella Baron; Aldo Scarpa; Christophe Adrie; Anne Janin; Stefano Barbi; Jean Kister; Philippe Rouyer-Fessard; Roberto Corrocher; Philippe Leboulch; Yves Beuzard
Journal of the Pancreas | 2002
Andrew A. Gumbs; Claudio Bassi; Patrick S. Moore; Massimo Falconi; Isabella Frigerio; Antonella Baron; Lorenzo Piemonti; Irvin M. Modlin; Aldo Scarpa
Human Mutation | 2000
Patrick S. Moore; Gildas Rigaud; Antonella Baron; Aldo Scarpa
Tumori | 2002
Antonella Baron; Aldo Scarpa
Gastroenterology | 2001
Vito D. Corleto; S. Ciardi; Ottavia De Luca; A. Moretti; Francesco Panzuto; Antonella Baron; Giuseppe Zamboni; Aldo Scarpa; Cinzia Azzoni; Cesare Bordi; Gianfranco Delle Fave