Antonietta Cerulli

Phenolics from Castanea sativa leaves and their effects on UVB-induced damage

Abstract The phytochemical investigation of the methanol extract of the leaves of Castanea sativa Mill., source of the Italian PGI (Protected Geographical Indication) product ‘Marrone di Roccadaspide’ (Campania region) afforded as main compounds crenatin (1), chestanin (2), gallic acid (3), cretanin (4), 5-O-p-coumaroylquinic acid (5), p-methylgallic acid (6) and quercetin-3-O-glucoside (7). To quantify the isolated compounds a LC-ESI(QqQ)MS method working with a very sensitive and selective mass tandem experiment called Multiple Reaction Monitoring (MRM) has been developed. Moreover the antioxidant capacity by TEAC assay and the ability of compounds 1–7 to protect HaCaT human keratinocytes from UVB-induced damage has been investigated.

Metabolite profiling of “green” extracts of Corylus avellana leaves by 1H NMR spectroscopy and multivariate statistical analysis

HIGHLIGHTSCorylus avellana leaves were extracted by “eco‐friendly” extraction protocols.Total phenolic content of extracts was measured by Folin‐Ciocalteu assay.Metabolite profiles of the extracts were obtained by 1H NMR experiments.Metabolite variation among the extracts was evaluated using PCA and PLS‐DA analysis.The antioxidant activity of the extracts was evaluated by DPPH, ABTS tests and in vitro pyocyanin assay. ABSTRACT Corylus avellana L. (Betulaceae) leaves, consumed as infusion, are used in traditional medicine, for the treatment of hemorrhoids, varicose veins, phlebitis, and edema due to their astringent, vasoprotective, and antiedema properties. In previous works we reported from the leaves of Corylus avellana cv. “Tonda di Giffoni” diarylheptanoid derivatives, a class of plant secondary metabolites with a wide variety of bioactivities. With the aim to give an interesting and economically feasible opportunity to C. avellana leaves as source of functional ingredients for pharmaceutical and cosmetic formulations, “green” extracts were prepared by employing “eco‐friendly” extraction protocols as maceration, infusion and SLDE‐Naviglio extraction. Metabolite profiles of the extracts were obtained by 1H NMR experiments and data were processed by multivariate statistical analysis to highlight differences in the extracts and to evidence the extracts with the highest concentrations of bioactive metabolites. Based on the NMR data, a total of 31 compounds were identified. The metabolite variation among the extracts was evaluated using Principle Component Analysis (PCA) and Partial Least Squares‐Discriminant Analysis (PLS‐DA). Furthermore, the total phenolic content of the extracts was measured by Folin‐Ciocalteu colorimetric assay and the antioxidant activity of extracts was assayed by the spectrophotometric tests DPPH• and ABTS and by an in vitro test based on the evaluation of cellular reactive oxygen species production stimulated by pyocyanin.

Isolation, identification, quantification and antioxidant activity of secondary metabolites from coastal Brazilian herbs

Unesp Univ Estadual Paulista Julio de Mesquita Fi, Lab Biodiversidade & Sustentabilidade, Botucatu, SP, Brazil