António Carlos Valera

Chronic mercury exposure in Late Neolithic/Chalcolithic populations in Portugal from the cultural use of cinnabar

Cinnabar is a natural mercury sulfide (HgS) mineral of volcanic or hydrothermal origin that is found worldwide. It has been mined prehistorically and historically in China, Japan, Europe, and the Americas to extract metallic mercury (Hg0) for use in metallurgy, as a medicinal, a preservative, and as a red pigment for body paint and ceramics. Processing cinnabar via combustion releases Hg0 vapor that can be toxic if inhaled. Mercury from cinnabar can also be absorbed through the gut and skin, where it can accumulate in organs and bone. Here, we report moderate to high levels of total mercury (THg) in human bone from three Late Neolithic/Chalcolithic (5400–4100 B.P.) sites in southern Portugal that were likely caused by cultural use of cinnabar. We use light stable isotope and Hg stable isotope tracking to test three hypotheses on the origin of mercury in this prehistoric human bone. We traced Hg in two individuals to cinnabar deposits near Almadén, Spain, and conclude that use of this mineral likely caused mild to severe mercury poisoning in the prehistoric population. Our methods have applications to bioarchaeological investigations worldwide, and for tracking trade routes and mobility of prehistoric populations where cinnabar use is documented.

The population genomics of archaeological transition in west Iberia: Investigation of ancient substructure using imputation and haplotype-based methods

We analyse new genomic data (0.05–2.95x) from 14 ancient individuals from Portugal distributed from the Middle Neolithic (4200–3500 BC) to the Middle Bronze Age (1740–1430 BC) and impute genomewide diploid genotypes in these together with published ancient Eurasians. While discontinuity is evident in the transition to agriculture across the region, sensitive haplotype-based analyses suggest a significant degree of local hunter-gatherer contribution to later Iberian Neolithic populations. A more subtle genetic influx is also apparent in the Bronze Age, detectable from analyses including haplotype sharing with both ancient and modern genomes, D-statistics and Y-chromosome lineages. However, the limited nature of this introgression contrasts with the major Steppe migration turnovers within third Millennium northern Europe and echoes the survival of non-Indo-European language in Iberia. Changes in genomic estimates of individual height across Europe are also associated with these major cultural transitions, and ancestral components continue to correlate with modern differences in stature.

The Transition to Agriculture in South-Western Europe: New Isotopic Insights from Portugal's Atlantic Coast

Abstract For the past 15 years, a succession of stable isotope studies have documented the abrupt dietary transition from the Mesolithic to the Neolithic in Western and Northern Europe. Portugal, with its Late Mesolithic shell middens and burials apparently coexisting with the earliest Neolithic, further illustrates the nature of that transition. Individuals from Neolithic contexts there had significantly different diets to their Mesolithic counterparts. No evidence was found for a transitional phase between the marine-oriented Mesolithic subsistence regimes and the domesticated, terrestrial Neolithic diet. Two later Neolithic individuals, however, showed evidence for partial reliance on marine or aquatic foods. This raises questions about the possible persistence of marine dietary regimes beyond the Mesolithic period. This article is followed by a brief note by Mary Jackes and David Lubell.

The Population Genomics Of Archaeological Transition In West Iberia

We analyse new genomic data (0.05-2.95x) from 14 ancient individuals from Portugal distributed from the Middle Neolithic (4200-3500 BC) to the Middle Bronze Age (1740-1430 BC) and impute genomewide diploid genotypes in these together with published ancient Eurasians. While discontinuity is evident in the transition to agriculture across the region, sensitive haplotype-based analyses suggest a significant degree of local hunter-gatherer contribution to later Iberian Neolithic populations. A more subtle genetic influx is also apparent in the Bronze Age, detectable from analyses including haplotype sharing with both ancient and modern genomes, D-statistics and Y-chromosome lineages. However, the limited nature of this introgression contrasts with the major Steppe migration turnovers within third Millennium northern Europe and echoes the survival of non-Indo-European language in Iberia. Changes in genomic estimates of individual height across Europe are also associated with these major cultural transitions, and ancestral components continue to correlate with modern differences in stature. Author Summary Recent ancient DNA work has demonstrated the significant genetic impact of mass migrations from the Steppe into Central and Northern Europe during the transition from the Neolithic to the Bronze Age. In Iberia, archaeological change at the level of material culture and funerary rituals has been reported during this period, however, the genetic impact associated with this cultural transformation has not yet been estimated. In order to investigate this, we sequence Neolithic and Bronze Age samples from Portugal, which we compare to other ancient and present-day individuals. Genome-wide imputation of a large dataset of ancient samples enabled sensitive methods for detecting population structure and selection in ancient samples. We revealed subtle genetic differentiation between the Portuguese Neolithic and Bronze Age samples suggesting a markedly reduced influx in Iberia compared to other European regions. Furthermore, we predict individual height in ancients, suggesting that stature was reduced in the Neolithic and affected by subsequent admixtures. Lastly, we examine signatures of strong selection in important traits and the timing of their origins.

Ceramics, Marbles and Stones in the Light of Neutrons: Characterization by Various Neutron Methods

In this chapter we give a brief overview of neutron based analytical investigations applied to study archaeological ceramics, and different types of stones. Since the vast majority of archaeological objects are made of ceramics and various stones—all are of geological origin—, one of the key objectives of these studies to determine the origin of raw material. This research is called provenance research, and a wide range of neutron based methods are applicable in it. Following a very basic, user-oriented description of the methods, we introduce examples from our everyday practice. The examples are about provenance of prehistoric stone tools, about the sources of 4th–3rd c. B.C. millennium limestone idols found in the South of Portugal, as well as about the characterization of 15th–16th c. A.D. Inka pottery. A very unique application of combined neutron techniques was aimed to determine the inner content of an Eighteenth Dynasty Egyptian sealed vessel. In addition, investigations of samples from different epochs and characterization of marbles are presented.