Antonio Danieli
University of Salento
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Publication
Featured researches published by Antonio Danieli.
Fish & Shellfish Immunology | 2003
Tiziano Verri; L. Ingrosso; Rita Chiloiro; Antonio Danieli; V. Zonno; Pietro Alifano; Nicla Romano; Giuseppe Scapigliati; Sebastiano Vilella; Carlo Storelli
Naked circular plasmid DNA containing the cytomegalovirus (CMV)-promoter-driven lacZ reporter gene (pCMV-LacZ) was injected in the epaxial muscle of gilthead sea bream (Sparus aurata). A mosaic pattern of expression of beta-galactosidase (beta-gal) in the myofibres at the site of injection was visualised by in situ histochemical staining using 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside. As measured by o-nitrophenyl-beta-D-galactopyranoside assay, beta-gal enzymatic activity was found to steadily increase for at least 50 days post injection (p.i.) in pCMV-LacZ-injected muscle. In parallel, foreign DNA was detected by polymerase chain reaction in injected muscles (but not in other tissues) up to 60 days p.i., persisting most probably in an extrachromosomal, non-replicative, circular form. Neither beta-gal activity nor pCMV-LacZ-related amplification products were found 90 days p.i. Antibodies against beta-gal were demonstrated in pCMV-LacZ-injected fish sampled 45 days p.i. The results suggest that intramuscular delivery of foreign genes represents a realistic approach for DNA vaccine technology for the prevention of infectious diseases in gilthead sea bream.
The Journal of Experimental Biology | 2003
Michele Maffia; Antonia Rizzello; Raffaele Acierno; Tiziano Verri; M. Rollo; Antonio Danieli; Frank Döring; Hannelore Daniel; Carlo Storelli
SUMMARY H+/peptide cotransport was studied in brush-border membrane vesicles (BBMV) from the intestine of the haemoglobinless Antarctic teleost Chionodraco hamatus by monitoring peptide-dependent intravesicular acidification with the pH-sensitive dye Acridine Orange. Diethylpyrocarbonate-inhibited intravesicular acidification was specifically achieved in the presence of extravesicular glycyl-L-proline (Gly-L-Pro) as well as of glycyl-L-alanine (Gly-L-Ala) and D-phenylalanyl-L-alanine (D-Phe-L-Ala). H+/Gly-L-Pro cotransport displayed saturable kinetics, involving a single carrier system with an apparent substrate affinity (Km,app) of 0.806±0.161 mmol l-1. Using degenerated primers from eel and human (PepT1) transporter sequence, a reverse transcription-polymerase chain reaction (RT-PCR) signal was detected in C. hamatus intestine. RT-PCR paralleled kinetic analysis, confirming the hypothesis of the existence of a PepT1-type transport system in the brush-border membranes of icefish intestine. Functional expression of H+/peptide cotransport was successfully performed in Xenopus laevis oocytes after injection of poly(A)+ RNA (mRNA) isolated from icefish intestinal mucosa. Injection of mRNA stimulated D-Phe-L-Ala uptake in a dose-dependent manner and an excess of glycyl-L-glutamine inhibited this transport. H+/peptide cotransport in the Antarctic teleost BBMV exhibited a marked difference in temperature optimum with respect to the temperate teleost Anguilla anguilla, the maximal activity rate occurring at approximately 0°C for the former and 25°C for the latter. Temperature dependence of icefish and eel intestinal mRNA-stimulated uptake in the heterologous system (oocytes) was comparable.
Journal of Biotechnology | 2015
Lidia De Riccardis; Antonia Rizzello; Alessandra Ferramosca; Emanuela Urso; Francesca De Robertis; Antonio Danieli; Anna Maria Giudetti; Giorgio Trianni; Vincenzo Zara; Michele Maffia
Multiple sclerosis (MS) is a chronic inflammatory autoimmune demyelinating disease of the central nervous system. There are four clinical forms of MS, the most common of which is characterized by a relapsing remitting course (RRMS). The etiology of MS is unknown, but many studies suggested that genetic, environmental and infectious agents may contribute to the development of this disease. In experimental autoimmune encephalomyelitis (EAE), the animal model for MS, it has been shown that CD4(+) T cells play a key role in MS pathogenesis. In fact, these cells are able to cross the blood-brain barrier and cause axonal damage with neuronal death. T cell activation critically depends on mitochondrial ATP synthesis and reactive oxygen species (ROS) production. Interestingly, lots of studies linked the oxidative damage arising from mitochondrial changes to neurodegenerative disorders, such as MS. Based on these evidences, this work focused on the metabolic reprogramming of CD4(+) T cells in MS subjects, being this cell population directly implicated in pathogenesis of disease, paying attention to mitochondrial function and response to oxidative stress. Such aspects, once clarified, may open new opportunities for a therapeutic metabolic modulation of MS disorder.
Zoological Studies | 2010
Ermelinda Prato; Antonio Danieli; Michele Maffia; Francesca Biandolino
The Journal of Experimental Biology | 2000
Tiziano Verri; Michele Maffia; Antonio Danieli; Martina Herget; Uwe Wenzel; Hannelore Daniel; Carlo Storelli
Research in Veterinary Science | 2008
A.M. Bakke-Mckellep; M. Sanden; Antonio Danieli; Raffaele Acierno; Gro Ingunn Hemre; Michele Maffia; Åshild Krogdahl
Aquaculture Nutrition | 2007
Gro Ingunn Hemre; A. Sagstad; A.M. Bakke-Mckellep; Antonio Danieli; Raffaele Acierno; Michele Maffia; M. Frøystad; Åshild Krogdahl; M. Sanden
American Journal of Physiology-regulatory Integrative and Comparative Physiology | 1997
Michele Maffia; Tiziano Verri; Antonio Danieli; M. Thamotharan; M. Pastore; G. A. Ahearn; Carlo Storelli
Archive | 2012
Ermelinda Prato; Antonio Danieli; Michele Maffia; Francesca Biandolino
Cellular and Molecular Neurobiology | 2012
Emanuela Urso; D. Manno; Antonio Serra; Alessandro Buccolieri; Antonia Rizzello; Antonio Danieli; Raffaele Acierno; Benedetto Salvato; Michele Maffia