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Dive into the research topics where Antonio Guerrieri is active.

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Featured researches published by Antonio Guerrieri.


Analytica Chimica Acta | 1996

Copper dispersed into polyaniline films as an amperometric sensor in alkaline solutions of amino acids and polyhydric compounds

Innocenzo G. Casella; Tommaso R. I. Cataldi; Antonio Guerrieri; Elio Desimoni

A chemically modified electrode composed of copper microparticles dispersed into a polyaniline (PANI) film was investigated as an amperometric sensor of scarcely electroactive compounds possessing -OH and -NH2 groups. Glassy carbon was used as an electrode material and modified firstly by a PANI film, then allowed to stand in contact with a solution of copper ions, and finally, the electroreduction was done at −0.3 V. The electrochemical behaviour of the resulting modified electrode in alkaline medium was examined by cyclic voltammetry and flow-injection amperometry. Using some representative compounds, the effect of copper loading and pH on the electrode response was investigated. Constant-potential amperometric detection was applied in conjunction with anion-exchange chromatographic (AEC) separations of amino acids and carbohydrates. At an applied potential of 0.55 V vs. AgAgCl, the detection limits (SN = 3) for all analytes investigated ranged 5–15 pmol, and the linear dynamic range was three–four orders of magnitude above the detection limits. The resulting modified electrode was found to retain 95% of its initial response in flowing streams for 3 h of operating time.


Biosensors and Bioelectronics | 1998

Electrosynthesized non-conducting polymers as permselective membranes in amperometric enzyme electrodes : a glucose biosensor based on a co-crosslinked glucose oxidase/overoxidized polypyrrole bilayer

Antonio Guerrieri; G.E. De Benedetto; Francesco Palmisano; Pier Giorgio Zambonin

A glucose amperometric biosensor based on glucose oxidase immobilized on an overoxidized polypyrrole (PPyox) platinum modified electrode, by glutaraldehyde co-crosslinking with bovine serum albumine, is described. The advantages of covalent immobilization techniques (e.g. high loading and long-term stability of the enzyme) are coupled with the excellent interferent rejection of electrosynthesized non-conducting polymers. The sensor showed an apparent Michaelis-Menten constant of 16 +/- 0.8 mM, a maximum current density of 490 microA/cm2 and a shelf lifetime of at least 3 months. Ascorbate, urate, cysteine and acetaminophen at their maximum physiological concentrations produced a glucose bias in the low micromolar range. Flow-injection response was linear up to 20 mM glucose with typical sensitivity of 84.0 +/- 1.5 nA/mM. The sensor was tested for glucose determination of untreated serum samples from both normal and diabetic subjects; results of amperometric assay compared well with those obtained by a standard enzymatic-colorimetric method.


Fresenius Journal of Analytical Chemistry | 1992

Interference-free glucose sensor based on glucose-oxidase immobilized in an overoxidized non-conducting polypyrrole film

D. Centonze; Antonio Guerrieri; C. Malitesta; Francesco Palmisano; Pier Giorgio Zambonin

SummaryAn amperometric glucose sensor is described; it is based on glucose oxidase immobilized in an overoxidized non-conducting polypyrrole membrane. The overoxidation process of polypyrrole produces a permselective, antifouling membrane capable of rejecting ascorbate, urate, acetaminophen and cysteine, as well as proteins and other surface active components typically present in serum. The amperometric assay of glucose in serum correlates well with an established routine procedure based on an enzymatic-colorimetric method.


Biosensors and Bioelectronics | 1993

An interference-free biosensor based on glucose oxidase electrochemically immobilized in a non-conducting poly(pyrrole) film for continuous subcutaneous monitoring of glucose through microdialysis sampling

Francesco Palmisano; D. Centonze; Antonio Guerrieri; Pier Giorgio Zambonin

A glucose biosensor, based on glucose oxidase immobilized in a non-conducting (overoxidised) polypyrrole film, is described which proved practically immune from faradaic interference arising from endogeneous (ascorbate, urate, cysteine) and exogeneous (acetaminophen) electroactive interferents. The bias introduced in the measurement of 5 mM glucose by the given interferents at their maximum physiological levels never exceeded 2% which is, by far, the lowest value ever reported. The biosensor has been used for continuous subcutaneous monitoring of glucose in a rabbit implanted with a microdialysis probe. The potential and limits of this approach are discussed.


Analyst | 2002

A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts

Antonio Guerrieri; L. Monaci; Maurizio Quinto; Francesco Palmisano

A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCl to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38 +/- 4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.


Analyst | 1995

Amperometric sensor for choline and acetylcholine based on a platinum electrode modified by a co-crosslinked bienzymic system

Antonio Guerrieri; Giuseppe De Benedetto; Francesco Palmisano; Pier Giorgio Zambonin

A fast-response and sensitive amperometric biosensor for choline and acetylcholine based on choline oxidase and acetylcholinesterase immobilized on a platinum electrode by co-crosslinking with bovine serum albumin and glutaraldehyde has been developed. The immobilization procedure proved fast and simple, not requiring dedicated manipulation. Detection limits were in the sub-micromolar range and linearity extended over three concentration decades. After one month of discontinuous use, 70% of the initial sensitivity was still observed. The response time was around 1 s for both analytes making the developed biosensor a suitable detector for flow injection systems and high-performance liquid chromatography.


Electroanalysis | 2000

Permselective Behavior of an Electrosynthesized, Nonconducting Thin Film of Poly(2‐naphthol) and Its Application to Enzyme Immobilization

Rosanna Ciriello; Tommaso R. I. Cataldi; D. Centonze; Antonio Guerrieri

The electrooxidation of 2-naphthol in phosphate buffer at pH 7 leads to the formation of a nonconducting polymer of poly(2-naphthol) on a platinum electrode. Such a resulting thin film displays an interesting permselective behavior, which proved useful in minimizing the interference of ascorbate, acetaminophen, cysteine, and urate sample molecules. Electrochemical detection in flowing streams was used to investigate the relevance of permselectivity for sensor development. Nonconducting poly(2-naphthol) film demonstrated useful also as a novel permselective membrane for glucose oxidase immobilization. The glucose response time, t0.95, evaluated in batch addition experiments, was lower than 4 s. The calibration plot was linear up to 15 mM of glucose with a sensitivity of 2.2 nA/mM.


Biosensors and Bioelectronics | 2009

Permselective and enzyme-entrapping behaviours of an electropolymerized, non-conducting, poly(o-aminophenol) thin film-modified electrode: a critical study.

Antonio Guerrieri; Rosanna Ciriello; D. Centonze

Non-conducting polymeric films synthesised by the electrooxidation of o-aminophenol on a platinum electrode in acetate or phosphate buffer displayed an interesting permselective behaviour, which proved valuable in minimising the electrochemical interferences from ascorbate, acetaminophen, cysteine and urate sample molecules in amperometric detection mode. The electrosynthesis of poly(o-aminophenol) (p(oAP)) film showed also useful as permselective membrane for enzyme immobilization as demonstrated by the production of an interference-free glucose oxidase biosensor. In this respect, the glucose response time, t(0.95), evaluated in batch addition experiments, was lower than 5s while the calibration curve was linear up to 10mM of glucose with a sensitivity of 69.7nA/mM. Both the permselective behaviour and the enzyme-entrapping property of the film were critically compared with the relevant studies until now reported. With respect to the sophisticated but complex approaches described elsewhere, this study shows that simply a proper optimization of p(oAP) electrosynthesis and its permselective behaviour is the key to improve significantly the selectivity of the resulting analytical devices.


Fresenius Journal of Analytical Chemistry | 1994

Electrochemically prepared glucose biosensors : kinetic and faradaic processes involving ascorbic acid and role of the electropolymerized film in preventing electrode-fouling

D. Centonze; Antonio Guerrieri; Francesco Palmisano; L. Torsi; Pier Giorgio Zambonin

The influence is discussed of ascorbic acid (AA) on the response of a glucose biosensor based on glucose oxidase immobilized in electropolymerized poly(ophenylenediamine) (PPD) or overoxidized poly(pyrrole) (oxPPy) films on a Pt electrode. The kinetics of the homogeneous reaction between AA and H2O2 has been investigated by two independent methods and found to be too slow to influence the response of typical glucose biosensors. Therefore, the decrease in the sensor response, observable when working in batch under typical experimental conditions, can in no way be ascribed to a depletion of H2O2, which is produced in the biocatalytic cycle via the homogeneous reaction with AA. While the purely additive Faradaic interference can practically be nullified by both entrapping membranes, electrode fouling by electro-oxidation products of AA (responsible for the observed decrease in glucose sensitivity) might still represent a problem when working with PPD based biosensors. In this respect the permeability characteristics of the film markedly influence the magnitude of the observed phenomena. The oxPPy film achieves the goal of completely eliminating ascorbate without blocking the access of glucose to the immobilized enzyme.


Analytica Chimica Acta | 1991

Simultaneous determination of γ-aminobutyric acid and polyamines by o-phthalaldehyde—β-mercaptoethanol precolumn derivatization and gradient elution liquid chromatography with electrochemical detection

Pier Giorgio Zambonin; Antonio Guerrieri; Taddeo Rotunno; Francesco Palmisano

Abstract A method for the simultaneous determination of γ-aminobutyric acid, putrescine, spermine, spermidine and cadaverine is described. A precolumn derivatization procedure utilizing o -phthalaldehyde—β-mercaptoethanol is employed to form electroactive isoindole derivatives, which are separated by reversed-phase gradient elution chromatography and detected amperometrically (+650 mV vs. Ag/AgCl) at a glassy carbon electrode. By careful optimization of the experimental conditions, the baseline drift during the gradient run could be contained within 0.5 nA. On-column detection limits are typically in the low pmol range and linear responses in the 5–200 pmol injected range. The procedure has been applied to rat brain, kidney and liver tissues after homogenization with perchloric acid.

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