Antonio Lidón

Responses of five Mediterranean halophytes to seasonal changes in environmental conditions

We have studied the responses to changing environmental conditions of five halophytes in a Mediterranean salt marsh, during a 2-year period. Salt tolerance in succulent dicotyledonous halophytes is mostly dependent on compartmentalisation of toxic ions in vacuoles and biosynthesis of osmolytes for osmotic adjustment – mechanisms that appear to be constitutive in the most tolerant taxa – while monocots avoid excessive ion transport to the plant aerial parts. Contrary to what has been described for salt treatments under artificial conditions, the selected halophytes are not affected by oxidative stress in their natural habitat, and do not need to activate antioxidant defence mechanisms.

Environmentally induced changes in antioxidant phenolic compounds levels in wild plants

Different adverse environmental conditions cause oxidative stress in plants by generation of reactive oxygen species (ROS). Accordingly, a general response to abiotic stress is the activation of enzymatic and non-enzymatic antioxidant systems. Many phenolic compounds, especially flavonoids, are known antioxidants and efficient ROS scavengers in vitro, but their exact role in plant stress responses in nature is still under debate. The aim of our work is to investigate this role by correlating the degree of environmental stress with phenolic and flavonoid levels in stress-tolerant plants. Total phenolic and antioxidant flavonoid contents were determined in 19 wild species. Meteorological data and plant and soil samples were collected in three successive seasons from four Mediterranean ecosystems: salt marsh, dune, semiarid and gypsum habitats. Changes in phenolic and flavonoid levels were correlated with the environmental conditions of the plants and were found to depend on both the taxonomy and ecology of the investigated species. Despite species-specific differences, principal component analyses of the results established a positive correlation between plant phenolics and several environmental parameters, such as altitude, and those related to water stress: temperature, evapotranspiration, and soil water deficit. The correlation with salt stress was, however, very weak. The joint analysis of all the species showed the lowest phenolic and flavonoid levels in the halophytes from the salt marsh. This finding supports previous data indicating that the halophytes analysed here do not undergo oxidative stress in their natural habitat and therefore do not need to activate antioxidant systems as a defence against salinity.

Composted organic wastes from the pharmaceutical and agro-food industries induce soil bioactivity and nodulation in alfalfa

BACKGROUND Environmentally friendly agriculture needs to reduce the use of synthetic fertilizers and to reclaim nutrients from organic wastes. In this study the effect of five doses (0, 12, 24, 48 and 96 t ha(-1) ) of two two-phase olive mill waste (TPOMW)-based composts on the bioactivity and chemical characteristics of an agricultural soil and their potential to fertilize alfalfa (Medicago sativa) and stimulate nodulation were assessed during a two-year incubation experiment. The two composts were prepared either with the olive mill waste alone (compost A), which served as control, or mixed with a liquid fatty-proteinaceous hydrolyzate waste (FPH) from the pharmaceutical industry (compost AH). RESULTS Compost AH resulted in greater N immobilization than compost A because the former supplied the soil with easily degradable C and N, which increased microbial biomass and activity. Both compost mineralizations during the first year of incubation supplied the soil with more nutrients (mainly N), more so with A than with AH. Nevertheless, plant growth was similar in soils amended with either A or AH. Both composts induced nodulation similarly and the highest dose (96 t ha(-1) ) increased the formation of nodules by a factor of 11 compared with the four lower doses. CONCLUSION TPOMW serves as an effective ground material for co-composting with liquid wastes such as FPH. TPOMW supplies key nutrients and stimulates nodulation in alfalfa.

Compartmental model for nitrogen dynamics in citrus orchards

A simple model that represents the soil nitrogen dynamics in a citrus orchard is studied. This model consists of several compartments of organic and inorganic nitrogen representing the main processes that occur among the compartment of the soil column. Some of these processes are coupled to the carbon dynamics in the soil, thus, the evolution of organic carbon in the soil has been also described in the model. The dependence of these processes with soil moisture requires the coupling of a nitrogen model with a model of soil water dynamics. A compartmental model for the water has been used to simulate the dynamics of water in the root profile. The proposed model has been used to predict the soil mineral nitrogen content and the nitrate leaching in a citrus orchard placed in the area of Valencia and the obtained results have been compared with the results obtained with the nitrogen component of the widely used Leaching Estimation and Chemistry Model (LEACHM).

Ecohydrological-Based Forest Management in Semi-arid Climate

The role of forests on the provision and regulation of non-marketed ecosystem services is well known (Thorsen et al. 2014). This is especially important in areas like the Mediterranean, where protective forests play a major role against soil erosion and degradation, landscape quality and stabilization of the hydrological cycle. Socio-economic and cultural changes affecting rural society from the 1960s have produced a demographic decline, and with it, an abandonment of rural activities, leading to an expansion and densification of forest and scrub. Forest encroachment may decrease the streamflow from upper catchments (Gallart and Llorens 2004); this study reports a decrease in average annual flow of major Spanish rivers between 37 and 59%, partly explained by the densification of upstream forests, and increasing interception loss. Moreover, some Mediterranean basins (e.g. Segura and Jucar in Spain) present very serious problems of water scarcity, because of a combination of low/irregular rainfall and high rates of evapotranspiration, that has resulted in overuse of groundwater resources (Estrela et al. 2000a, b). These problems may even endanger urban water supply (approx. 15% of the total water supply in Spain). In addition, the Mediterranean region is already suffering some significant impacts of the climate change, such as longer dry seasons, or lower soil moisture content (Giorgi and Lionello 2008; Garcia-Ruiz et al. 2011). All these issues have raised concern about the importance of forests and water interactions in the Mediterranean (Birot et al. 2011).