Antonio Mezzogiorno

Fibrillogenesis and cytotoxic activity of the amyloid-forming apomyoglobin mutant W7FW14F

The apomyoglobin mutant W7FW14F forms amyloid-like fibrils at physiological pH. We examined the kinetics of fibrillogenesis using three techniques: the time dependence of the fluorescence emission of thioflavin T and 1-anilino-8-naphthalenesulfonate, circular dichroism measurements, and electron microscopy. We found that in the early stage of fibril formation, non-native apomyoglobin molecules containing β-structure elements aggregate to form a nucleus. Subsequently, more molecules aggregate around the nucleus, thereby resulting in fibril elongation. We evaluated by MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) the cytotoxicity of these aggregates at the early stage of fibril elongation versus mature fibrils and the wild-type protein. Similar to other amyloid-forming proteins, cell toxicity was not due to insoluble mature fibrils but rather to early pre-fibrillar aggregates. Propidium iodide uptake showed that cell toxicity is the result of altered membrane permeability. Phalloidin staining showed that membrane damage is not associated to an altered cell shape caused by changes in the cytoskeleton.

Oral benign fibrous histiocytoma: two case reports

Fibrous histiocytoma is a benign soft tissue tumour arising as a fibrous mass everywhere in the human body. The involvement of the oral cavity is rare. We report two cases of benign fibrous histiocytoma that localized in the oral cavity. The clinical and histological features of the lesion are reported. Finally, a literature revision of this pathology at the level of the oral cavity is reported.

Secondary Müllerian System: An Atypical Case of Tumor Originating From Vestigial Müllerian Cells Embedded in the Peritoneum

Clinical Practice Points Tumors originating from vestigial Mullerian cells embedded in the peritoneum could give rise to a primitive, peritoneal, mixed neoplasia with a predominant myoid differentiative trait and a double mesenchymal component. When possible, the most adequate treatment involves the en bloc resection of the mass together with the pelvic, parietal, and diaphragmatic peritoneum, and omentum, uterus, and annexes. The surgical treatment should be completed with the removal of the bulky right and left iliac lymph nodes. The components of the Mullerian ducts that do not undergo processes of differentiation and organogenesis constitute the secondary Mullerian system, from which various proliferative disorders might originate, such as endometriosis, endosalpingiosis, and endocervicosis. Epithelial-like cancers can also originate from the secondary Mullerian system, such a serous, mucinous, and endometrioid hystotypes.

Cysts and Pseudocysts of the Oral Cavity: Revision of the Literature and a New Proposed Classification

This article includes a comprehensive and up-to-date review on the cysts of the oral cavity. Several classifications of odontogenic (OC) and non-odontogenic (non-OC) oral cysts and the surrounding regions have been proposed. We suggest a new critical classification based on an established relationship between anatomical area, histological origin and clinical behavior (frequency, rate of recurrence, malignant potential). Moreover, the differential cytokeratin (CKs) expression of the various cysts is reported as epithelium-specific markers of differential diagnosis. Finally, issues related to differential diagnosis and therapeutic approaches of the cysts included in the two groups are described.

History of the Nephron

The many progressive achievements in knowledge about the structure and function of the nephron – the kidney’s main morpho-functional unit – have always been influenced by philosophical theories, which for a long time have swung between vitalism and mechanism. From Galen’s first imaginative vitalistic hypothesis, the scientific thought about the kidney’s structure/function gradually moved to the iatro-mechanistic view promoted by Borelli, Bellini and Malpighi, to then meet Bowman’s bright vitalistic-based theory. But Bowman’s theory was rather soon replaced by novel yet still mechanistic theories proposed first by Ludwig and then Starling. Ludwig based his scientific approach to knowledge of renal function upon physics and chemistry; Starling eventually developed a ‘colloid-osmotic’ theory.