Antonio Peroglio Deiro

Repeatability and reproducibility of retinal thickness measurements with spectral-domain optical coherence tomography using different scan parameters.

Purpose: To evaluate repeatability (test–retest) and reproducibility of retinal thickness measurements using spectral-domain optical coherence tomography with different densities of A-scans per B-scan and different frames per B-scan for real-time averaging. Methods: Twelve healthy subjects were analyzed by spectral-domain optical coherence tomography. Raster lines analysis with 19 B-scans over the examined area centered on the macula was performed. Images were acquired both in standard-density (768 A-scans/B-scan) and high-density (1,536 A-scans/B-scan) modalities. Moreover, images were acquired using 2 and 20 frames/B-scan for real-time averaging. Each analysis was repeated twice to test for repeatability. Results: Intersession repeatability was good for all studied analysis protocols, with Lin concordance correlation coefficient values ranging between 0.88 and 1.00. Reproducibility assessment showed consistent retinal thickness measurements using variable scan density, with Bland–Altman limits of agreement of −6 &mgr;m to 6 &mgr;m in the central area. Reproducibility assessment showed consistent retinal thickness measurements using different number of frames used in the real-time averaging process, with Bland–Altman limits of agreement of −8 &mgr;m to 4 &mgr;m in the central area. Conclusion: Spectral-domain optical coherence tomography is a very reliable tool for central retinal thickness assessment. Changes in the number of A-scans/B-scan and in frames used for real-time averaging do not affect repeatability and reproducibility.

Spectral domain-optical coherence tomography and fundus autofluorescence findings in a case of purtscher-like retinopathy.

PURPOSE The purpose of this study is to describe spectral domain-optical coherence tomography and autofluorescence images in a case of bilateral Purtscher-like retinopathy. METHODS This is an observational case report of a 31-year-old man suffering with Purtscher-like retinopathy postpancreatitis. RESULTS In the acute phase, spectral domain-optical coherence tomography showed hyperreflectivity in the inner retinal layers, corresponding to cotton-wool exudation as a result of precapillary ischemia. The follow-up using spectral domain-optical coherence tomography showed that the inner retina is the most involved layer by ischemic damage in the acute phase. In the regression phase, signs of suffering in the outer retinal layers became visible as well, although after the resolution of the pathology, there was no evident spectral domain-optical coherence tomography abnormality in the photoreceptors layers. Autofluorescence images after resolution showed hyperautofluorescent vessels affected by ischemia as a possible result of long-lasting retention of intraretinal blood. CONCLUSION Spectral domain-optical coherence tomography and autofluorescence are sensitive and noninvasive imaging modalities to evaluate retinal alterations after Purtscher-like retinopathy. Spectral domain-optical coherence tomography confirms that ischemia causes an alteration primarily in the inner retinal layers, but the process also involves the outer retinal layers.

New Insights Into the Development and Progression of Geographic Atrophy After Full Thickness Autologous Choroidal Graft

Purpose To investigate if the contiguity between native and transplanted retinal pigment epithelium (RPE) represents a protective factor against the progression of atrophy after autologous choroidal graft. In addition, the changes in fundus autofluorescence (FAF) in the contiguous and noncontiguous RPE areas were explored. Methods The first postoperative reliable FAF image was selected and divided into sectors based on the characteristics of the RPE at the edge of the graft. The sectors were categorized into three groups: contiguous RPE, noncontiguous RPE, and not classifiable. The area of RPE atrophy, inside and outside the graft, was measured for each sector at baseline, one, three, and five years of follow-up. The FAF pattern outside the graft was evaluated for every sector at baseline and during the follow-up. Results Nineteen patients met the inclusion criteria and were included in this study. Trend analysis showed that the atrophy progression outside the graft was statistically significant in areas where native and transplanted RPE were noncontiguous (P < 0.0001) yet not so in contiguous areas (P < 0.058). Inside the graft, both groups showed an increase in atrophy over time. In addition, the noncontiguous group developed more severe increased FAF patterns compared with the contiguous group. Conclusions RPE contiguity after autologous choroidal graft seems to be a protective factor against atrophy progression, whereas any area of damaged or absent RPE tends to enlarge over time. This may suggest that the transplantation of an RPE sheet is more likely to be effective than an RPE cell suspension.

Morphological changes in the diseased retina on a healthy choroid-retinal pigment epithelial complex after full macular translocation for exudative age-related macular degeneration

To describe the change in the retinal morphology after full macular translocation (FMT) for exudative age‐related macular degeneration (AMD) and identify predictive factors for the visual outcome.