Antonio Vetrani

Fine-needle cytology and flow cytometry immunophenotyping and subclassification of non-Hodgkin lymphoma: A critical review of 307 cases with technical suggestions

Flow cytometry (FC) is a useful adjunct to fine‐needle aspiration cytology (FNC) in evaluating lymphoproliferative disorders. The authors present a critical review of 307 lymph nodal and extra lymph nodal lymphoproliferative disorders that were diagnosed with FNC and FC.

Impact of endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) in lymph nodal and mediastinal lesions: A multicenter experience

Endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) is an established procedure in lung cancer (LC) staging and in the diagnosis of mediastinal masses. Most of the experiences reported refer to single specialized centers where dedicated teams of endoscopists and pathologists perform the procedure. We report the EUS‐FNA experience of a cooperation group involving clinicians and cytopathologists from three hospitals. Fifty‐seven consecutive EUS‐FNA of mediastinal nodes in LC patients, eight mediastinal and two subdiaphragmatic masses were collected in 3 years. EUS‐FNA was performed by two endoscopists and three experienced pathologists. On‐site evaluation was performed in all cases by the three cytopathologists. Lymph node negative cases underwent surgery, which confirmed the cytological diagnoses but also detected two false negatives. Four of the 10 EUS cytological diagnoses of mediastinal and subdiaphragmatic masses were histologically confirmed. All EUS diagnoses were blindly reviewed by three pathologists to assess intra and interpersonal reproducibility. FNA‐EUS diagnoses were: 10 inadequate (17%), 10 negative (17%), 4 suspicious (7%) and 33 positive (59%). Diagnoses of mediastinal and subdiaphragmatic masses were: relapse of lung carcinoma (3), mesenchimal tumor not otherwise specifiable (3), gastrointestinal stromal tumor (GIST) (1), esophageal carcinoma (2) and paraganglioma (1). The sensitivity attained was 85% and the specificity 100%; revision of the slides demonstrated a significant diagnostic reproducibility of the three cytopathologists (P < 0.5). The sensitivity and specificity attained were similar to those reported in the literature suggesting that experienced cytopathologists and endoscopists from different institutions can employ the same procedure reaching comparable results. Diagn. Cytopathol. 2010.

Fine‐needle aspiration biopsy and flow cytometry immunophenotyping of lymphoid and myeloproliferative disorders of the spleen

Flow cytometry (FC) is a useful adjunct to fine‐needle aspiration biopsy (FNAB) in the evaluation of lymphoproliferative disorders. The application of FC to FNAB of the spleen (sFNAB) is reported.

Fine-needle aspiration cytology in the follow-up of Hodgkin lymphoma.

Hodgkin lymphoma (HL) is characterized by long survival and risk of relapse and second neoplasm. The aim of this study is to evaluate the possibility of improving the accuracy of fine‐needle cytology (FNC) in HL follow‐up using Power Doppler ultrasound (US) assistance and immediate microscopic evaluation (ICE).

Benign chondroblastoma on fine‐needle aspiration smears: A seven‐case experience and review of the literature

We report seven cases of chondroblastoma (CB) of bone, diagnosed by Fine‐Needle Aspiration Cytology (FNAC), and confirmed by histomorphological examination. The concurrence of some cytomorphologic findings — mononucleated cells, multinucleated cells, and intercellular chondroid substance — unequivocally suggested the cytological diagnosis of CB. We also reviewed the literature on this topic in order to discuss morphological criteria and the importance of needle size. The differential diagnosis between CB, Giant Cell Tumor of Bone, and Eosinophilic Granuloma is further discussed. Diagn. Cytopathol. 2015;43:734–738.