Anurag Payasi

Ethylenediaminetetraacetic acid: A non antibiotic adjuvant enhancing Pseudomonas aeruginosa susceptibility

Pseudomonas aeruginosa is an opportunistic bacterium which has been shown to have multi-drug resistance against fluoroquinolones, β-lactams, and aminoglycosides. In this investigation, we studied the effect of different concentrations of ethylenediaminetetraacetic acid (EDTA) on MexA-MexB-OprM efflux pump and subsequent changes in susceptibility and expression. Next, we examined, the expression of mexAB gene following treatment with half of minimum inhibitory concentration (MIC) of drugs. Our results revealed that 10 mM EDTA significantly reduced MIC of all drugs; moreover, the higher reduction (8 fold) was observed with CSE1034. MexA and MexB expression was down regulated at 2.93 and 3.21 fold, respectively with 10 mM EDTA. When the same concentration of EDTA was incorporated with drugs, the CSE1034 down regulates 5.64 and 5.94 fold expression of mexA and mexB, respectively. Moreover, meropenem treated groups exhibited 2.63 and 3.12 fold down regulation in the expression of the genes. However, treatment with piperacillin plus tazobactam, amoxicillin plus clavulanate, cefoperazone plus sulbactam and imipenem plus cilastatin did not produce changes in the expression of MexAB-OprM. Hence, CSE1034 could be one of the best choices to treat infections caused by microorganisms that overexpressed MexAB-Opr-M as compared to other drugs. Furthermore, use of EDTA disodium at appropriated concentrations can be regarded as a safe strategy to fight against the menace provided by the efflux pumps.

Role of EDTA and CSE1034 in curli formation and biofilm eradication of Klebsiella pneumoniae: a comparison with other drugs

Role of EDTA and CSE1034 in curli formation and biofilm eradication of Klebsiella pneumoniae : a comparison with other drugs

Role of CSE1034 in bacterial lipids and polysaccharides involved in biofilm formation: a comparison with other drugs

In the present investigation minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) and CSE1034 (Sulbactomax) was compared with piperacilline+tazobactam, amoxyclave, ceftriaxone, ceftriaxone+sulbactam and cefoperazone+ sulbactam in planktonic and sessile cells of Acinetobacter baumannii, Enterobacter cloacae and Pseudomonas aeruginosa . MICs were determined by broth microdilution method with a final inoculum size of 10 6 cfu/ml of plaktonic cells. MBECs were measured using a calgary biofilm device method to establish a co-relation with biofilm breaking efficacy of different drugs. The MICs for CSE1034 ranged from 0.5 to 4.0 µg/ml and for other antibacterial drugs ranged from 2 to 32 µg/ml. The MBEC for CSE1034 ranged from 8 to 16 µg/ml and for other antibacterial agents, it ranged from 64 to 4096 µg/ml. The CSE1034 exhibited approximately 5 logs reduction in the number of bacteria present in biofilm when compared with other antibacterial agents. When total lipid and total polysaccharide contents were compared, CSE1034 showed 90 and 84% reduction, respectively. The enhanced efficacy of CSE1034 in the eradication of biofilm infection is due to presence of EDTA which helps in the destabilizing of the barriers responsible for the development of biofilm as well as antibiotic resistance. In conclusion, combining of ceftriaxone+sulbactam with EDTA can significantly reduces the MIC and MBEC values against selected organisms. Hence, CSE1034 could be one of the best choices to eradicate the biofilm caused by these organisms.