Arezoo M. Ardekani

Hydrodynamic mechanisms of cell and particle trapping in microfluidics

Focusing and sorting cells and particles utilizing microfluidic phenomena have been flourishing areas of development in recent years. These processes are largely beneficial in biomedical applications and fundamental studies of cell biology as they provide cost-effective and point-of-care miniaturized diagnostic devices and rare cell enrichment techniques. Due to inherent problems of isolation methods based on the biomarkers and antigens, separation approaches exploiting physical characteristics of cells of interest, such as size, deformability, and electric and magnetic properties, have gained currency in many medical assays. Here, we present an overview of the cell/particle sorting techniques by harnessing intrinsic hydrodynamic effects in microchannels. Our emphasis is on the underlying fluid dynamical mechanisms causing cross stream migration of objects in shear and vortical flows. We also highlight the advantages and drawbacks of each method in terms of throughput, separation efficiency, and cell viability. Finally, we discuss the future research areas for extending the scope of hydrodynamic mechanisms and exploring new physical directions for microfluidic applications.

Numerical investigation of particle–particle and particle–wall collisions in a viscous fluid

The dynamics of particle-particle collisions and the bouncing motion of a particle colliding with a wall in a viscous fluid is numerically investigated. The dependence of the effective coefficient of restitution on the Stokes number and surface roughness is analysed. A distributed Lagrange multiplier-based computational method in a solid-fluid system is developed and an efficient method for predicting the collision between particles is presented. A comparison between this method and previous collision strategies shows that the present approach has some significant advantages over them. Comparison of the present methodology with experimental studies for the bouncing motion of a spherical particle onto a wall shows very good agreement and validates the collision model. Finally, the effect of the coefficient of restitution for a dry collision on the vortex dynamics associated with this problem is discussed.

Dynamics of bead formation, filament thinning and breakup in weakly viscoelastic jets

The spatiotemporal evolution of a viscoelastic jet depends on the relative magnitude of capillary, viscous, inertial and elastic stresses. The interplay of capillary and elastic stresses leads to the formation of very thin and stable filaments between drops, or to ‘beads-on-a-string’ structure. In this paper, we show that by understanding the physical processes that control different stages of the jet evolution it is possible to extract transient extensional viscosity information even for very low viscosity and weakly elastic liquids, which is a particular challenge in using traditional rheometers. The parameter space at which a forced jet can be used as an extensional rheometer is numerically investigated by using a one-dimensional nonlinear free-surface theory for Oldroyd-B and Giesekus fluids. The results show that even when the ratio of viscous to inertio-capillary time scales (or Ohnesorge number) is as low as Oh ~ 0.02, the temporal evolution of the jet can be used to obtain elongational properties of the liquid.

Low-Reynolds-number swimming at pycnoclines

Microorganisms play pivotal functions in the trophic dynamics and biogeochemistry of aquatic ecosystems. Their concentrations and activities often peak at localized hotspots, an important example of which are pycnoclines, where water density increases sharply with depth due to gradients in temperature or salinity. At pycnoclines organisms are exposed to different environmental conditions compared to the bulk water column, including reduced turbulence, slow mass transfer, and high particle and predator concentrations. Here we show that, at an even more fundamental level, the density stratification itself can affect microbial ecology at pycnoclines, by quenching the flow signature, increasing the energetic expenditure, and stifling the nutrient uptake of motile organisms. We demonstrate this through numerical simulations of an archetypal low-Reynolds-number swimmer, the “squirmer.” We identify the Richardson number—the ratio of buoyancy forces to viscous forces—as the fundamental parameter that quantifies the effects of stratification. These results demonstrate an unexpected effect of buoyancy on low-Reynolds-number swimming, potentially affecting a broad range of abundant organisms living at pycnoclines in oceans and lakes.

Unsteady motion of two solid spheres in Stokes flow

This study is concerned with the unsteady motion of two solid spherical particles in an unbounded incompressible Newtonian flow. The background flow is uniform and can be time dependent. In addition, the particle Reynolds numbers 2aVa∕ν and 2bVb∕ν, based on characteristic particles velocities Va and Vb, are assumed to remain small throughout the motion. Here, a and b denote the particle radii and ν is the kinematic viscosity of the fluid. Two approximate methods are employed in order to calculate the unsteady force exerted on each particle. In the first approach, a simplified method of reflections in combination with the point-force method is employed. In the second approach, a simplified method of reflections combined with Burger’s unsteady flow solution is considered. The forces due to the background flow and the disturbed flow created by the presence of particles are treated separately. The equation of motion for each particle is derived and some special cases are presented in detail including the moti...

Effect of solid boundaries on swimming dynamics of microorganisms in a viscoelastic fluid

We numerically study the effect of solid boundaries on the swimming behavior of a motile microorganism in viscoelastic media. Understanding the swimmer-wall hydrodynamic interactions is crucial to elucidate the adhesion of bacterial cells to nearby substrates which is precursor to the formation of the microbial biofilms. The microorganism is simulated using a squirmer model that captures the major swimming mechanisms of potential, extensile, and contractile types of swimmers, while neglecting the biological complexities. A Giesekus constitutive equation is utilized to describe both viscoelasticity and shear-thinning behavior of the background fluid. We found that the viscoelasticity strongly affects the near-wall motion of a squirmer by generating an opposing polymeric torque which impedes the rotation of the swimmer away from the wall. In particular, the time a neutral squirmer spends at the close proximity of the wall is shown to increase with polymer relaxation time and reaches a maximum at Weissenberg number of unity. The shear-thinning effect is found to weaken the solvent stress and therefore, increases the swimmer-wall contact time. For a puller swimmer, the polymer stretching mainly occurs around its lateral sides, leading to reduced elastic resistance against its locomotion. The neutral and puller swimmers eventually escape the wall attraction effect due to a releasing force generated by the Newtonian viscous stress. In contrast, the pusher is found to be perpetually trapped near the wall as a result of the formation of a highly stretched region behind its body. It is shown that the shear-thinning property of the fluid weakens the wall-trapping effect for the pusher squirmer.

Microfluidic fabrication of shape-tunable alginate microgels: Effect of size and impact velocity

We report on a capillary-based microfluidic platform for the fabrication of non-spherical sodium alginate microgels. The sodium alginate droplets were crosslinked off-chip in a mixture of barium acetate and glycerol solution. Novel morphologies such as tear drop, lamp-like, mushroom-like, double-dimpled and bowl-like microgels were fabricated by controlling the size, impact velocity (at the crosslinking solution/oil interface), and concentration of sodium alginate solution. We monitored the microscale deformation process in situ at the interface and proposeed a deformation mechanism resulting in unique morphologies. Additionally, we constructed microgel superstructures by assembling the non-spherical alginate microgels to spherical poly(N-isopropylacrylamide) (pNIPAAm) microgels via electrostatic interaction.

Two spheres in a free stream of a second-order fluid

The forces acting on two fixed spheres in a second-order uniform flow are investigated. When α1+α2=0, where α1 and α2 are fluid parameters related to the first and second normal stress coefficients, the velocity field for a second-order fluid is the same as the one predicted by the Stokes equations while the pressure is modified. The Stokes solutions given by Stimson and Jeffery [Proc. R. Soc. London, Ser. A 111, 110 (1926)] for the case when the flow direction is along the line of centers and Goldman et al. [Chem. Eng. Sci. 21, 1151 (1966)] for the case when the flow direction is perpendicular to the line of centers are utilized and the stresses and the forces acting on the particles in a second-order fluid are calculated. For flow along the line of centers or perpendicular to it, the net force is in the direction that tends to decrease the particle separation distance. For the case of flow at arbitrary angle, unequal forces are applied to the spheres perpendicularly to the line of centers. These forces ...

Particle-wall collision in a viscoelastic fluid

In this study, we present experimental results on particle-wall collision in viscoelastic fluids. A sphere is released in a tank filled with poly(ethylene-oxide) (PEO) mixed with water with varying concentrations up to 1.5 %. The effect of Stokes and Deborah numbers on the rebound velocity of a spherical particle colliding onto a wall is considered. It has been observed that the slope at which the coefficient of restitution increases with Stokes number is smaller for higher Deborah numbers. Higher rebound occurs for higher PEO concentration at the same stokes number. However, the results for the coefficient of restitution in polymeric liquids can be collapsed together with the Newtonian fluid behaviour if one defines the Stokes number based on the local strain rate.

Fabrication of Shape Controllable Janus Alginate/pNIPAAm Microgels via Microfluidics Technique and Off-Chip Ionic Cross-Linking

A novel method to fabricate shape controllable alginate/pNIPAAm complex microgels is reported. Monodisperse alginate/pNIPAAm droplets are created via microfluidics and cross-linked in different concentrations of hot glycerol/barium acetate water solutions. By changing the initial droplet size and glycerol concentration of the collecting solution, the resultant microgel shape and surface details can be systematically tuned. High-speed imaging is used to visualize and explain the microgel formation process.