Armand Abramovici

Induction of Atypical Prostatic Hyperplasia in Rats by Sympathomimetic Stimulation

Prostatic innervation may participate in its homeostasis and growth. α‐Adrenergic inhibition alleviates clinical symptoms in benign prostatic hyperplasia. However, the prostatic effect of adrenergic agonists has not been investigated. This study deals with the prostatic effect of subchronic sympathomimetic stimulation.

Clinical and pathological findings in prostates following intravesical bacillus Calmette-Guerin instillations

The prostates of 36 patients who were treated with intravesical bacillus Calmette-Guerin were evaluated by digital rectal examination and transrectal ultrasonography. When abnormal palpatory and/or ultrasonographic findings were detected, core needle biopsies from the suspicious areas were performed. Of the 36 patients 20 underwent biopsies of the prostate. Pathological findings revealed typical granulomas in 8 patients (3 caseating and 5 noncaseating multifocal granulomas). Nonspecific chronic prostatitis was noted in 4 patients and benign prostatic hyperplasia was noted in 8. The number of bacillus Calmette-Guerin instillations ranged from 6 to 19. The interval from initiation of therapy to biopsy ranged from 1.5 to 14.5 months. Caseating granulomas were found during the early course of bacillus Calmette-Guerin instillations (1.5 to 3.0 months), whereas noncaseating granulomas were detected at later stages (4 to 14.5 months). These findings present a high incidence of granuloma formation in patients treated with intravesical bacillus Calmette-Guerin. The duration of therapy is a determinant factor in the induction of granuloma type.

Molecular structure-teratogenicity relationships of some fragrance additives

The embryotoxic effect of some flavour additives was tested on young chick embryos. A certain correlation between the molecular structure of a compound and its teratogenic expression was observed. The presence of an unsaturated bond at C2 near a carbonyl function was found to be more effective than its saturated analogs or the alcoholic derivatives. Short chains or semicyclic structures, as well as additional -OH groups might favour the embryotoxic impact. An additional finding was that the teratogenic effect of a compound seems to be modulated by the dose related lethal effect. A uniform phenotypic expression of the malformation was the rule for all the tested compounds. The possible interreaction between the liposoluble unsaturated carbonyl compounds and the lipid constituents of the embryonic cell membrane is offered as a mechanism for the teratogenic activity of some flavour additives.

The teratogenic effect of cosmetic constituents on the chick embryo.

Cosmetic products have a more extended usage than drugs, especially if we consider their wide and habitual usage by healthy people, particularly women. The scent is the particular constituent in these preparations which make their reputation and thus their success on the market; the characteristic bouquet of perfumes, creams, soaps, shampoos, sprays, etc., is obtained by adding different mixtures of essential oils which bear the name of the plants and flowers (1) from which they are extracted in small quantities (rose, citronella, lemon, bergamot oils). Currently, they are produced industrially at high purity; these industrial products are called by their original name, to which the suffix of alcoholic or aldehyde derivate is added (citral = lemon oil, citronellal = citronella oil, etc.).

Placental ultrasonographic biochemical and histochemical studies in human fetuses affected with Niemann-Pick disease type A

Placental ultrasonographic, bio- and histochemical studies were performed on four unrelated fetuses affected with Niemann-Pick disease Type A, following prostaglandin-induced abortion at about the 19th week of gestation. An accumulation of sphingomyelin in the placentae of affected fetuses indicates the essential role of the enzyme sphingomyelinase, even during the early stages of gestation. A fair correlation between histochemical localization of sphingomyelin in the placentae and ultrasonographic findings was found, indicating the value of ultrasonic echo wave information in the diagnosis of metabolic disorders.

Citral and testosterone interactions in inducing benign and atypical prostatic hyperplasia in rats

Citral is a monoterpene in wide use as an aromatic supplement in the cosmetics and food industries. Previous studies in our laboratory have shown that cutaneous application of citral on adolescent rats may induce benign prostatic hyperplasia (BPH)-like and even atypical hyperplastic changes in the ventral lobes. In the present study we investigate the possible interactions between citral and serum testosterone levels on the induction of hyperplastic changes in the ventral prostate of adolescent rats. In addition, the study includes a comparative analysis of normal intact rats showing circadian variations of serum testosterone levels and rats in whom this rhythmic pattern was abolished either by excessive supplementation of exogenous androgen or by castration. Our results demonstrate an induction of benign as well as atypical prostatic hyperplasia following citral application. The most severe atypical changes were noted in the citral-treated rats with high serum testosterone levels. Although the mechanism of action of citral is yet unknown, the present results suggest a synergism between citral and testosterone resulting in hyperplastic changes in the rat ventral prostate. In addition, the results reconfirm that serum testosterone levels fluctuate according to a circadian rhythm in intact young and adolescent male rats. The application of citral tends to lower the morning circadian peaks, and the circadian pattern was abolished in orchiectomized rats and in those treated with testosterone implants.

Histochemical and quantitative biochemical changes in mucopolysaccharides of the term placenta of the diabetic rat

Full-term placentae of rats with streptozotocin-induced diabetes were analysed histochemically and biochemically for changes in mucopolysaccharide content. The experimental diabetes induced severe cystic degeneration in the spongiosa zone of the placenta and this was accompanied by extracellular deposition of hyaluronate and neutral polysaccharides. The labyrinth zone was affected to a lesser extent. Biochemical analysis also revealed quantitative differences in the total contents of mucopolysaccharides (MPS) between the two zones of the placenta in both diabetic and normal placentae. The spongiosa had a higher total mucopolysaccharide content than the labyrinth. Polysaccharides of low molecular weight were always predominant. The amount of hyaluronate exceeded the quantitites of sulphated mucopolysaccharides which were found in relatively small amounts in both placental zones. In diabetic placentae there was a two- to threefold increase, which was more pronounced in the spongiosa. The increase was observed among all types of MPS. A close correlation between the accumulation of MPS in the placenta and the degree of maternal hyperglycaemia was found. The placentae of the three streptozotocin-resistant animals showed neither histological nor biochemical changes. It is suggested that the accumulation of MPS in the diabetic placenta might be a consequence of a shift in carbohydrate metabolism induced by hyperglycaemia or a persistence of the fetal metabolic features as a part of a retarded maturational process of the placenta.

Histochemical localization of acidic glycosaminoglycans in normal human placentae.

First-trimester and term placentae were studied histochemically with alcian blue stain before and after specific enzyme treatments. A specific deposition of glycosaminoglycans was detected in the villous stroma, fetal blood vessels and on the surface of the syncytiotrophoblast, the latter being discontinuous and of variable thickness. Treatments of sections with hyaluronidases from Streptomyces and from bovine testes and with chondroitinase ABC indicated the presence of (1) chondroitin sulphates and hyaluronic acid mainly in the stroma; (2) heparan sulphate and dermatan sulphate associated with villous fetal blood vessels and the intervillous surface of the syncytiotrophoblast. It is suggested that the location of individual glycosaminoglycans could be related to their functions with regard to the maintenance of the structural integrity of the placenta by preventing its compression and to their involvement in blood anticoagulation and in lipid metabolism.

Effects of senescence and citral on neuronal vacuolar degeneration in rat pelvic ganglia.

A significant part of the morbidity in elderly men involves pelvic organs and their autonomic neural regulation. Environmental stimuli also impair the structure and function of pelvic organs. One of these factors is citral, a widely-used cosmetic fragrance constituent, which causes severe prostatic hyperplasia in rats. In this study, we assessed the effect of topical administration of citral (30 days) on the morphology of pelvic ganglia (PG) in young adult and old Wistar rats. Neuronal vacuolar degeneration with preserved nuclei of PG neurons was observed in untreated senescent, but not young rats. Citral significantly increased the rate of vacuolated neurons in old rats (from 3 to 14%), but only slightly in young ones (from 0 to 0.5-0.3%). Similar lesions were not found in inferior cervical or celiac ganglia, in either group. This shows that environmental stimuli enhance age-related processes of vacuolar neuronal degeneration in PG, and may contribute to the dysfunction of pelvic organs in the elderly.

Postmortem Changes in the Human Carotid Body

Heath and his colleagues have described three varieties of Type I cells based upon differences in nuclear morphology (Heath et al., 1970); this is at odds with the situation in experimental animals. Pallot et al. (1992) examined rat carotid bodies fixed at various times after death of the animal. Their data showed that, with increasing delay in fixation of the carotid body, first Type I cells with dark nuclei became apparent and with further delay cells with pyknotic nuclei appeared. Here we correlate the percentage occurrence of clear, dark and pyknotic nucleated cells in the human carotid body with the delay between death of the patient and fixation of the tissue.