Aroni Sattler

Nosema ceranae has been present in Brazil for more than three decades infecting Africanized honey bees.

Until the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light microscopy, without ultrastructural analysis) wrongly identified N. ceranae as N. apis. Although we can conclude that N. ceranae has been affecting Africanized honeybees in Brazil for at least 34 years, the impact of this pathogen remains unclear.

An improved method for the detection and presumptive identification of Paenibacillus larvae subsp. larvae spores in honey

SUMMARY One of the most important diseases that attacks honey bees (Apis spp.) in the larval stage is American foulbrood (AFB), for which Paenibacillus larvae subsp. larvae is the causative agent. American foulbrood is considered an exotic bee disease in Brazil because it has not yet been detected in the country. Feeding honey containing spores of P. I. larvae to bee larvae can transmit AFB, hence imported honey could spread this disease to Brazil. Since the disease is widely spread in some Latin American countries, including one bordering Brazil, the Brazilian authorities, and beekeepers, are concerned about the risk of introduction of AFB to Brazil. Accordingly, an improved method of detecting spores of P. I. larvae in honey has been developed. The method is based on concentrating spores by centrifugation, inactivating vegetative cells and then inoculating onto a newly developed selective solid medium: Paenibacillus larvae subsp. larvae agar (PLA). Suspect colonies are then selected and cultures are confirmed by biochemical identification. The method was able to detect less than 10 viable spores of P. I. larvae per 1 ml of honey.

Essential minerals and inorganic contaminants (barium, cadmium, lithium, lead and vanadium) in dried bee pollen produced in Rio Grande do Sul State, Brazil

Like other beehive products, such as honey, royal jelly and propolis, bee pollen has attracted great interest because of the health benefits it can provide when consumed. Bee pollen has high contents of sugars and proteins and a low content of lipids, it is also a rich source of vitamins and other bioactive compounds, which makes it an attractive micronutrient supplement. However, few studies have investigated its composition. Therefore, the aim of this study was to characterize the essential minerals and inorganic contaminants present in bee pollen produced at apiaries in Rio Grande do Sul State, Brazil. Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) revealed the presence of 8 essential minerals (calcium, iron, copper, chromium, manganese, molybdenum, phosphorus and zinc) in the 5 analyzed samples; 6 of them were in sufficiently high amounts to meet dietary requirements. Of the 5 inorganic contaminants assessed (barium, cadmium, lithium, lead and vanadium), only cadmium was present at levels over the International Honey Commission’s standards. All bee pollen samples showed a high content of the 8 essential minerals. Contamination usually results from the use of pesticides, fertilizers and other chemicals in agriculture; thus, monitoring of its levels must be included in bee pollen analysis.

Genetic parameters for five traits in Africanized honeybees using Bayesian inference

Heritability and genetic correlations for honey (HP) and propolis production (PP), hygienic behavior (HB), syrup-collection rate (SCR) and percentage of mites on adult bees (PMAB) of a population of Africanized honeybees were estimated. Data from 110 queen bees over three generations were evaluated. Single and multi-trait models were analyzed by Bayesian Inference using MTGSAM. The localization of the hive was significant for SCR and HB and highly significant for PP. Season-year was highly significant only for SCR. The number of frames with bees was significant for HP and PP, including SCR. The heritability estimates were 0.16 for HP, 0.23 for SCR, 0.52 for HB, 0.66 for PP, and 0.13 for PMAB. The genetic correlations were positive among productive traits (PP, HP and SCR) and negative between productive traits and HB, except between PP and HB. Genetic correlations between PMAB and other traits, in general, were negative, except with PP. The study permitted to identify honeybees for improved propolis and honey production. Hygienic behavior may be improved as a consequence of selecting for improved propolis production. The rate of syrup consumption and propolis production may be included in a selection index to enhance honeybee traits.

Isolamento de esporos de Paenibacillus larvae subsp. larvae no Brasil

The objective of this work was to detect the presence of Paenibacillus larvae subsp. larvae spores in products from a warehouse located in Rio Grande do Sul State, Brazil, the identification of possible contamination sources, and the assessment of spores transference possibility from contaminated imported products from the warehouse to apiaries located in the surrounding area. Samples of imported pollen and bulk honey stocked in the warehouse, and honeycomb (brood, honey and pollen) from a healthy hive, honey from one apiary and adult bees were analyzed. Imported honey and pollen, and three groups of adult bees and the honey collected from the honeycomb resulted positive.

Stingless Bees as Alternative Pollinators of Canola

ABSTRACT Alternative pollinators can ensure pollination services if the availability of the managed or most common pollinator is compromised. In this study, the behavior and pollination efficiency of Apis mellifera L. and two species of stingless bees, Plebeia emerina Friese and Tetragonisca fiebrigi Schwarz, were evaluated and compared in flowers of Brassica napus L. ‘Hyola 61’. A. mellifera was an efficient pollinator when collecting nectar because it effectively touched the reproductive organs of the flower. In contrast, stingless bees were efficient pollinators only when collecting pollen. The number of pollen grains deposited on the stigma after a single visit by worker bees of the three species was greater than the number of grains resulting from pollination without the bee visits. On average, the three species deposited enough pollen grains to fertilize all of the flower ovules. A. mellifera and P. emerina had similar pollination efficiency because no significant differences were observed in the characteristics of the siliques produced. Although T. fiebrigi is also an effective pollinator, the seed mass produced by their pollination was lower. Native bees promoted similar rates of fruit set compared with A. mellifera. Thus, P. emerina has potential to be used for pollination in canola crops.

Report on the microbiota of Melipona quadrifasciata affected by a recurrent disease

Melipona quadrifasciata is an eusocial stingless bee traditionally used for honey production in Brazil. In the last decades, the species disappeared from the wild in Southern Brazil, being kept exclusively in managed colonies for commercial and recreational purposes. Stingless beekeepers from this region report annual losses of their colonies due to a syndrome of yet unknown causes. We investigate whether it is associated to pathogenic microorganisms already known to cause disease in bees. These results provide a starting point for future studies aimed at clarifying the relationship between the microbial community of stingless bees and their colony collapses.

A melissopalynological analysis of Apis mellifera L. loads of dried bee pollen in the southern Brazilian macro-region

Abstract Sixty-one samples of dried bee pollen collected from various apiaries in the southern Brazilian macro-region were examined to detect the botanical origin of the pollen and to enable more accurate product certification. The palynological analysis of the samples followed the standard methodology and was conducted without the use of acetolysis. The samples were washed once or twice with ethanol and then with water. The sediment obtained was homogenised in a water/glycerine solution for microscopic observation. The target number of pollen grains to be counted was 500 pollen grains or more per sample. The results demonstrated that 35 samples consisted of a unique pollen type representing more than 90% of the pollen sum (or more than 60% if no accessory pollen was present). These samples were considered monofloral. Several pollen types grouped in a sample were related to heterofloral pollen batches. The most frequent pollen types were of Mimosa scabrella, Eucalyptus, Andira, Machaerium, Myrcia and Piptocarpha. The results of the current study were related to the surrounding vegetation of the apiaries and reflected the resources available to the bees. Furthermore, these results are relevant to apicultural activities and are commercially significant.