Arthur L. Haskins

The estrogenic potential of estriol: A clinical and laboratory re-evaluation☆

Abstract The estrogenic effect of estriol was studied in humans through the observation of vaginal cytology, ferning of the cervical mucus, estrogen withdrawal bleedings, progestin withdrawal bleeding, and endometrial stimulation. The findings indicate that estriol possesses a low order of estrogenicity at 1 mg. a day. The relative efficacy of estriol is less than 10 per cent of stilbestrol. Specific polarity of estrogenic response in the human reproductive tract could not be demonstrated.

Oviductal sterilization with tantalum clips

Abstract Oviductal sterilization with tantalum clips was accomplished in 350 patients. The sterilization procedures were approached transabdominally in the puerperal patient and transvaginally in the interval patient. The technique in each instance was simple, and the operative complications were minimal. The pregnancy rate in the patients with abdominal puerperal oviductal sterilization was 11 per cent. The pregnancy rate in the patients with vaginal oviductal sterilization was 1.2 per cent.

Studies on the level of blood progesterone throughout the course of labor

Abstract Serum progesterone was measured during the course of induced human labor utilizing a protein-binding technique. The results demonstrate a consistent fall at the onset of true labor with a return to prelabor levels during the accelerated phase of labor. A second fall was seen during the postpartum period. The possible significance of these results is discussed.

The placental transfer of progesterone-4-C14 in human term pregnancy☆

Abstract Progesterone-4-C 14 was administered intravenously to human mothers at term. Determinations of radioactivity in the maternal and fetal organism before and after labor indicated a free diffusion of the radioactivity. The radioactivity was identified as progesterone and pregnanediol. The progesterone and pregnanediol plasma concentrations in the fetus were similar to those obtained in the mother.

CHIARI-FROMMEL SYNDROME: MEDROXYPROGESTERONE ACETATE THERAPY.

Abstract Three patients with Chiari-Frommel syndrome were treated successfully with luteoid suppressive therapy. There was evidence of ovulation in each patient. Two of the patients became pregnant and one has been delivered of a normal infant.

Quantitative assay of human chorionic gonadotropin: A comparison of immunoassay and bioassay

Abstract Three commercially available immunoassay techniques for chorionic gonadotropin determination were studied in our laboratory. The quantitative results were compared with the bioassay technique with which we were most familiar (the male frog test). Each immunoassay technique was specific and highly sensitive for chorionic gonadotropin. Concentration of the chorionic gonadotropin with kaolin adsorption procedures did not alter the effectiveness of the Pregnosticon or UCG test. Marked alteration of effectiveness in the Cravindex technique was noted following this procedure. Lyophilization of urine for HCG concentration was effective within circumscribed limits. There was excellent agreement in quantitation when the erythrocyte agglutination test of UCG test and Pregnosticon were compared. Quantitation with the Gravindex Procedure revealed significant variation from the results obtained with the other tests. Agreement between the immunoassay and biologic assay for chorionic gonadotropin was noted.

Uterine choriocarcinoma treated with operation and amethopterin

Abstract A case of metastatic uterine choriocarcinoma, proved by tissue biopsy, has undergone complete remission for more than one year following surgical excision of the primary tumor supplemented by a single course of protracted amethopterin therapy. The recommended amethopterin regimen was modified in this case for the reasons stated.

Metabolism of ovarian steroids in a patient with chronic familial anovulation

Abstract The intermediary metabolism of ovarian steroids was studied in a patient with chronic familial anovulation. The isolated metabolites demonstrated a predominance of metabolites containing the Δ5-3 β -ol configuration. Isolated in ovarian tissue were four unusual metabolites: 16αOH-estrone, 16αOH-dehydroepiandrosterone, 7 ketodehydroepiandrosterone, and estriol-16α, 17α.

Estrogenic potential of estriol. A comparison of estrone, estradiol, and estriol in aqueous and lipoid solvents with bioassay.

Abstract The comparative estrogenicity of estriol, estrone, and estradiol in a lipoid solvent was determined using the mouse modification of the Astwood uterine imbibition test. Estriol, estrone, and estradiol from doses of 0.001 mcg. to 20 mcg. induced similar uterine weight increases, thereby indicating similar or identical estrogenic potentials among the hormones. Comparson of estriol in oil with estriol in water indicated that at dosage levels from 0.001 mcg. to 0.1 mcg. yielded similar uterine responses. In doses above 0.1 mcg. the effectiveness of aqueous estriol is significantly less than estriol in peanut oil.

THE DIAGNOSTIC SIGNIFICANCE OF FETAL HEMOGLOBIN IN THE MATERNAL CIRCULATION DURING PREGNANCY

Transplacental hemorrhage is now known to occur. If it does it may be fatal or injurious to the fetus. By new technics it is now feasible to measure fetal hemoglobin in the maternal blood stream as proof of fetal blood loss into the maternal circulation.