Arthur N. Mason

The degradation of β-sitosterol by pseudomonas sp. NCIB 10590 under aerobic conditions

Abstract The bacterial degradation of β-sitosterol by Pseudomonas sp NCIB 10590 has been studied. Major biotransformation products included 24-ethylcholest-4-en-3-one, androsta-1,4-diene-3,17-dione, 3-oxochol-4-en-3-one-24-oic acid and 3-oxopregn-4-en-3-one-20-carboxylic acid. Minor products identified were 26-hydroxy-24-ethylcholest-4-en-3-one, androst-4-ene-3,17-dione, 3-oxo-24-ethylcholest-4-en-26-oic acid, 3-oxochola-1,4-dien-3-one-24-oic acid, 3-oxopregna-1,4-dien-3-one-20 carboxylic acid and 9α-hydroxyandrosta-1,4-diene-3,17-dione. Studies with selected inhibitors have enabled the elucidation of a comprehensive pathway of β-sitosterol degradation by bacteria.

The degradation of hyodeoxycholic acid by Pseudomonas spp. N.C.I.B. 10590

Abstract The microbial degradation of hyodeoxycholic acid by Pseudomonas spp. N.C.I.B. 10590 has been studied. Two major products and one minor product have been isolated and identified as 6α-hydroxyan-drosta-1, 4-dien-3,17-dione, 6α-hydroxy-3-oxopregna-1,4-dien-20-carboxylic acid and androsta-1,4-dien-3, 6,17-trione respectively. Two other minor products were isolated and evidence is given for the following structures: 6α-hydroxyandrost-4-en-3,17-dione and 6α-hydroxy-3-oxopregn-4-en-20-carboxylic acid. A possible paathway of hyodeoxycholic acid degradation is suggested.

The degradation of chenodeoxycholic acid by Pseudomonas Spp. N.C.I.B. 10590.

Abstract The microbial degradation of chenodeoxycholic acid by Pseudomonas spp. N.C.I.B. 10590 has been studied. Two major products have been isolated and identified as 7α-hydroxy-1,4-androstadiene-3,17-dione and 7α-hydroxy-3-oxo-1,4-pregnadiene-20-carboxylic acid. Two minor products were isolated and evidence is given for the following structures: 1,4,6-androstatriene-3,17-dione and 7α-hydroxy-3-oxo-4-pregnene-20-carboxylic acid. A possible pathway of chenodeoxycholic acid degradation is suggested.

Novel microbial transformation of deoxycholic acid

Deoxycholic acid has been converted into 12ξ-hydroxyandrosta-1,4-diene-3,17-dione by Pseudomonas sp. NCIB 10590.