Arzu Özlüer Hunt

Effects of Clove Oil and Eugenol on Anaesthesia and Some Hematological Parameters of European Eel Anguilla anguilla, L., 1758

Abstract Altun, T., Hunt, A.Ö. and Usta, F. 2006. Effects of clove oil and eugenol on anaesthesia and some hematological parameters of European eel Anguilla anguilla, L., 1758. J. Appl. Anim. Res., 30: 171–176. To investigate anaesthetic and some hematological effects of clove oil and eugenol for European eel, Anguilla anguilla, L., 1758, 0.0, 0.025, 0.05 and 0.075 ml/l of clove oil or 0.0, 2.250, 3.375 and 4.500 mi/l of eugenol were applied to fish (80.95±8.94 g). After fish was anaesthetized, half of fishes from each group were transferred into recovery tanks (to clean water), whereas, other half was retained there for next 48h. Induction time ranged between 3 and 5 min for clove oil and 2 and 5 min for eugenol. Fish reached to 5th anaesthesia stage in all experimental groups except control and the lowest concentration group of both anaesthetics. Reaching the fish to 8th stage of recovery took 60 min in all groups. Opercular rates of fish in test groups of clove oil and eugonal decreased slightly. During the study no mortality was observed. WBC counts decreased (P<0.05), but RBC counts, hematocrit value and glucose level increased by increasing dose (P<0.05). It is conclused that 0.050 ml/l of clove oil or 3.375 ml/l of eugenol can be accepted suitable to anaesthetize European eel of this size.

Mannan Oligosakkarit (MOS) İlaveli Yemlerle Beslenen Cyprinus carpio’larda Büyüme, Kas Kompozisyonu ve Lizozim Aktivitesinin Araştırılması

Bu calismada mannan oligosakkarit (MOS) ilaveli yemle beslenen Cyprinus carpio ’da buyume, vucut komposizyonu ve lizozim enzim aktivitesi arastirilmistir. Bu amacla baliklar, MOS’un %0.25 ve %0.50 oranlarini iceren yemlerle 60 gun sureyle beslenmislerdir. MOS’un buyume parametreleri (canli agirlik kazanci, spesifik buyume orani, yem cevirim orani) uzerine etkisinin incelendigi calismamizin sonucunda, %0.25 MOS ilavesi yapilan gruptaki baliklarda diger gruplardaki baliklara gore genel olarak daha iyi bir buyume performansi gozlenmis ve istatistiki olarak onemli bir fark belirlenmistir (P 0.05). MOS ilavesi yapilan gruplarda; kuru madde, lipit ve ham kul iceriklerindeki farkliliklarin onemli olmadigi (P>0.05), ancak ham protein iceriginin ise %0.50 MOS iceren diyetle beslenen grupta ise daha dusuk ve istatistiksel olarak fark yarattigini ortaya koymustur. MOS iceren yemlerle beslenen baliklarin serum lizozim aktivitesi, kontrol grubuna gore onemli duzeyde artmistir. Anahtar Kelimeler : Cyprinus carpio , mannan oligosakkarit, buyume, lizozim. Abstract An Investigation of Effects of Mannan Oligosaccharide on Growth, Body Composition, and Lysozyme Activity of Carp ( Cyprinus carpio ) In this study, Cyprinus carpio was fed with mannan oligosaccharide (MOS) supplemented diet (0.25% 0.50% the proportion of MOS) for 60 days. The effects of MOS on growth parameters (live weight gain, specific growth rate, feed convertion ratio) and lysozyme activity were investigated. Better growth performance was observed in 0.25% MOS group when compared to other groups (P 0.05). Dry matter, lipid and ash contents of MOS groups were not significant compared to control group. But, crude protein content was lower in 0.50% MOS group. Lysozyme activity was significantly increased (P<0.05) by MOS concentrations in two experimental groups when compared to control. Keywords : Cyprinus carpio , mannan oligosaccharide, growth, lysozyme.