Ashish Asrondkar

Antileishmanial potential of fused 5-(pyrazin-2-yl)-4H-1,2,4-triazole-3-thiols: Synthesis, biological evaluations and computational studies

A series of newer 1,2,4-triazole-3-thiol derivatives 5(a-m) and 6(a-i) containing a triazole fused with pyrazine moiety of pharmacological significance have been synthesized. All the synthesized compounds were screened for their in vitro antileishmanial and antioxidant activities. Compounds 5f (IC50=79.0µM) and 6f (IC50=79.0µM) were shown significant antileishmanial activity when compared with standard sodium stibogluconate (IC50=490.0µM). Compounds 5b (IC50=13.96µM) and 6b (IC50=13.96µM) showed significant antioxidant activity. After performing molecular docking study and analyzing overall binding modes it was found that the synthesized compounds had potential to inhibit L. donovani pteridine reductase 1 enzyme. In silico ADME and metabolic site prediction studies were also held out to set an effective lead candidate for the future antileishmanial and antibacterial drug discovery initiatives.

Microwave-assisted synthesis of novel 5-substituted benzylidene amino-2-butyl benzofuran-3-yl-4-methoxyphenyl methanones as antileishmanial and antioxidant agents.

A series of 5-substitutedbenzylideneamino-2-butylbenzofuran-3-yl-4-methoxyphenyl methanones is synthesized and evaluated for antileishmanial and antioxidant activities. Compounds 4f (IC50 = 52.0 ± 0.09 µg/ml), 4h (IC50 = 56.0 ± 0.71 µg/ml) and 4l (IC50 = 59.3 ± 0.55 µg/ml) were shown significant antileishmanial when compared with standard sodium stibogluconate (IC50 = 490.0 ± 1.5 µg/ml). Antioxidant study revealed that compounds 4i (IC50 = 2.44 ± 0.47 µg/ml) and 4l (IC50 = 3.69 ± 0.44 µg/ml) have shown potent comparable activity when compared with standard ascorbic acid (IC50 = 3.31 ± 0.34 µg/ml). Molecular docking study was carried out which replicating results of biological activity in case of initial hits 4f and 4h suggesting that these compounds have a potential to become lead molecules in drug discovery process. In silico ADME study was performed for predicting pharmacokinetic profile of the synthesised antileishmanial agents and expressed good oral drug like behaviour.

Synthesis of substituted 4-(3-chloro-2-oxo-4-phenylazetidin-1-yl)- 1, 5-dimethyl-2-phenyl-1, 2-dihydro-3H-pyrazol-3-one with Green Chemistry approach

The rate at which reaction proceeds and how yield does it provide, has always been a question of key importance in the field of research, carried out at both academic and industrial level. With the advent of microwave irradiation technology, it has now become possible to address this difficulty owing to its property to interact directly with molecules of reacting mixture without altering their molecular structure and thus providing a forward thrust to this whole time consuming process. The presented work compares the synthesis of derivatives of 4-aminoantipyrine with microwave irradiation and conventional method. It also addresses and attempts to find out the possession of anti-oxidant activity of the derivatives. The analysis of the synthesized derivatives was carried out using analytical techniques such as HPLC, FTIR.

Comparative studies on Synthesis of 2-Chloro-N-(2, 5-Dihydro-1, 3, 4-Thiadiazol-2-Yl) Acetamide using Microwave irradiation and Conventional techniques and their Antioxidant activity.

The advantages of Microwave irradiation technology in the field of organic chemistry have been long known as it facilitates accelerated reaction rates, higher yields with relatively less power consumption than classical techniques. The presented study compares aspects like reaction time and percentage yield of various 2- amino-1, 3, 4-thiadiazole derivatives when synthesized by both, conventional as well by microwave irradiation along with their characterization using techniques such as FTIR and HPLC. They have a very promising role in the field of medicinal chemistry owing to their anti-cancer and anti-fungal properties The study also aims to evaluate biological activity of the compounds a simple and inexpensive technique making use of 2,2-Diphenyl-1- picrylhydrazyl(DPPH) to establish their worth as potential anti-oxidants . Thus this combination of faster synthesis and cost effectiveness can significantly reduce the cost of production of various pharmaceutical products containing thiadiazole and/or its derivatives which are known to be clinically potent.

Synthesis of substituted Coumarin derivatives and their spectral Characterization

A series of some coumarin derivative have been synthesized by the interaction of 4-hydroxy coumarin with substituted benzaldehyde. The identities of these synthesized compounds have been established on the basis of chemical transformation and IR, 1 H NMR and Mass spectral studies. In the present study

Synthesis of 5-Aryl-3-(5-Bromo-3-Benzofuran-2-yl)-1-Pyrazole as Antimicrobial Agent.

In this work, we evaluate the biological activities of some new derivatives of benzofuran which can be used as effective anti-microbial agents. The recent reviews of literature have highlighted the attention of medicinal chemists because of their diverse biological activities and profound efficacy. Clinically potent benzofurans generated interest to construct a system which possesses anti-bacterial and anti-fungal activity. Furan and benzofuran are associated with wide spectrum of biological activity. In the view of these, an effort was made to check some synthesized compounds for their anti-microbial activity. In present study, pyrazoline derivatives were synthesized; 5-bromo-3-methyl acetophenone undergoes ring formation in presence of chloroacetone to form benzofuran which further forms chalcone on treatment with substituted benzaldehyde. This intermediate on treatment with hydrazine hydrate results into pyrazoline. Further it reacts with various benzoyl chlorides to form the titled product. Synthesized compounds have been confirmed on the basis of spectral studies and analytical data. All the compounds were screened for their in- vitro anti-bacterial activity against Gram positive Staphylococcus aureus ATCC3750 and Gram negative Salmonella typhi NCTC786, anti-fungal strains of Candida albicans ATCC10233 and Aspergillus niger ATCC

Synthesis of substituted 1, 2, 4-triazole derivatives by Microwave irradiation

Various substituted Triazole-Thiol containing different functional group have been synthesized by microwave method. The title product 1-((3H-indol-2-ylamino) methyl)-4-phenyl-4, 5-dihydro-1H-1, 2, 4- triazole-3-thiol is synthesized by using amino benzothiazole. The final structures have been established on the basis of their chemical analysis and spectral data. All micro-wave synthesized compounds results into good yield as compared to conventional method of which fluoro substituted compound shows maximum yield. Keyword: Benzothiazole, 1,2,4-triazole,Microwave irradiation etc