Ashish Kumar Sarker

Antimicrobial and Anticancer Activities of Ethyl Acetate Extract of Co-culture of Streptomyces sp. ANAM-5 and AIAH-10 Isolated From Mangrove Forest of Sundarbans, Bangladesh.

In this study we investigated the antimicrobial and anticancer activities of ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 isolated from soil of mangrove forest Sundarbans, Bangladesh. The antimicrobial activity of ethyl acetate extract was determined using broth-dilution method against Candida albicans, Saccharromyces cerevaceae and Aspergillus niger whereas anticancer activity was evaluated against Ehrlich Ascites Carcinoma (EAC) cells in Swiss albino mice with the dose of 50 mg/kg and 100 mg/kg body weight (i.p). The Minimum Inhibitory Concentrations (MIC) of ethyl acetate extract was found 32μg/ml against Candida albicans while 64 μg/ml against Saccharromyces cerevaceae and Aspergillus niger. The antineoplastic activity of the crude extract was increased in dose dependent manner with a significant value (p<0.01). Bacterial crude extract enhanced the mean survival time (MST) of tumor bearing mice at 71.79% and maximum cell growth inhibition was found 75.75 % with dose of 100 mg/kg body weight (i.p.). Our study revealed that ethyl acetate extract of co-culture of Streptomyces sp. ANAM-5 and AIAH-10 is an excellent source of antimicrobial and anticancer compounds which may become helpful to treat infections and cancer.

Isolation and characterization of a marine bacterium from Sundarbans, Bangladesh.

Aims: Marine environment is a vast but represents a largely untapped source for isolation of new microorganism having ability to produce novel bioactive compound(s ). The present study was designed with an aim to discover and identify marine bacterial strain producing antibacterial metabolites from marine soil samples of Sundarbans, Bangladesh. Study Design : We used serial dilution technique for isolation of marine b acteria, streak plate technique for screeninig of priliminery antibacterial activity, cover slip culture method for morphological characterization and the 16S rDNA sequencing approach for phylogenetic characterization .