Atif Riaz

Effect of Saccharomyces cerevisiae fermentation on the colorants of heated red beetroot extracts

The quality and flower yield of roses are directly dependent on the balanced application of macro and micronutrients. In the present study, foliar application of macro- and micronutrients was done after every 15 days when new emerging leaves had sprouted after pruning. The results reveal that plants treated with foliar application of micronutrients along with NPK showed significant increase in the growth characteristic like plant height, number of flowers plant-1, bud diameter, flower diameter, fresh and dry weight of flower, flower quality, flower stalk length compared to the application of NPK alone and untreated plants (control). Application of foliar fertilizer (NPK = 15:32:7 + micro power) and NPK (15:32:7) + chelated mix micronutrients gave the highest values compared to the other treatments in both cultivars. Cardinal responded well to micronutrients as compared to Whisky Mac in case of cultivars. Leaf total chlorophyll contents, vase life and the mineral contents (NPK) of leaves were significantly increased as a result of foliar application of micronutrients compared to the control treatment. It was concluded that application of micronutrients along with NPK could improve flower yield and quality of roses. Keywords : Chlorophyll content, NPK percentage, micronutrients, rose African Journal of Biotechnology Vol. 12(7), pp. 702-708

Exogenous 24-Epibrassinolide Elevates the Salt Tolerance Potential of Pea (Pisum sativum L.) by Improving Osmotic Adjustment Capacity and Leaf Water Relations

Salinity, as a wide spread problem around the globe, can drastically limits the crop growth and productivity. Therefore, there is a growing need to mitigate the drastic effects of this abiotic stress through the use some sustaiable short-term or long term approaches. An investigation was carried out to assess the potential role of 24-epibrassinolide (EBL) in mitigating the drastic effects of salt stress on growth, water relations, osmolytes, and ions accumulation of salt-tolerant and salt-sensitive pea genotypes. EBL treatment significantly altered the leaf water status and accumulation of osmolytes and various ions in salt-stressed [with sodium chloride (+NaCl)] plants. The plants of both salt-tolerant and salt-sensitive genotypes, subjected to +NaCl conditions had a significant reduction in plant fresh and dry weights, internodal distance, leaf area (LA), leaf water potential (w), leaf osmotic potential (o), leaf turgor potential (t), relative water content (RWC), number of seeds pod−1, seed weight, root/leaf inorganic osmolytes (Ca2+, K+ and Mg2+), while increased the levels of root/leaf proline, glycinebetaine, total free amino acids, total soluble sugars, sodium (Na+), and chloride (Cl−). There was non-significant effect of EBL on number of pods plant−1 both under saline and non-saline conditions. However, the foliar application of EBL significantly alleviated the deleterious effects of salinity by improving the plant biomass, water relations and concentration of organic/inorganic osmolytes and yield attributes. However, EBL minimized the drastic effects of salt stress by limiting the root/leaf Na+ and Cl− contents. It can be concluded that EBL efficiently alleviated the salinity-induced drastic effects by improving the water status of plant tissues, which led to salt dilution within plant tissues, thus minimized the ion-toxicity, ultimately elevated the plant growth by minimizing the toxic action of Na+ and Cl−. Secondly, EBL augmented the salt-tolerance capacity of tested pea genotypes by enhancing their osmotic adjustment potential, in terms of high accumulation of organic and inorganic osmolytes within leaf and root tissues.

Pre-planting Exogenous Application of Gibberellic Acid Influences Sprouting, Vegetative Growth, Flowering, and Subsequent Bulb Characteristics of 'Ad-Rem' Tulip

Tulip (Tulipa gesneriana L.) is a fascinating bulbous cut flower, and also used for potting, bedding, and border purpose. However, under the sub-tropical climatic conditions, quality tulip flower production is severely affected by its non-uniform bulb sprouting, lack or erratic flowering, small flower stalk, and short vase life. Hence, this study was performed to evaluate the potential of gibberellic acid (GA3) on sprouting, vegetative growth, flowering, flower quality, and vase life, including different bulb characteristics of ‘Ad-Rem’ tulip. Tulip bulbs were dipped in aqueous solution of different GA3 concentrations (0, 50, 75, 100, 125, and 150 mg L-1) for 30 min with Tween-20 (0.01%) as a surfactant. Tulip bulbs treated with 100 mg·L-1 GA3 sprouted in significantly less number of days, exhibited higher sprouting percentage, more plant height, leaf area, leaf chlorophyll, photosynthesis rate, flower stalk length, stalk diameter, and fresh and dry flower stalk weight. Moreover, 100 mg·L-1 GA3 application also decreased overall flower emergence time, increased flower size, produced attractive, glowing, and sturdy flowers with increased quality, and extended the vase life of ‘Ad-Rem’ tulip flowers up to 2.9-folds. However, 150 mg·L-1 GA3 application exhibited better results regarding daughter bulblets per clump with a subsequent increase in bulb diameter, and weight compared to other treatments. Additionally, significant correlation was also found among different growth, flower, and bulb characteristics of GA3-treated tulip plants. Hence, 100-150 mg·L-1 pre-planting exogenous application of GA3 may be effective for quality tulip production under the sub-tropical climatic conditions.

Microsatellite Analysis of Rosa damascena from Pakistan and Iran

For the perfume industry, Rosa damascena is the most important species used in the production of rose attar which is made by distilling volatile oils from the petals of flowers. It is also used widely in the manufacture of rose water, as a flavoring agent. Other species like Rosa gallica L., Rosa centifolia L., Rosa × borboniana Desp. and the rose cultivar Gruss an Teplitz also exhibit the fragrance that is sought by perfumeries in the world. The main rose oil producers in the world are Turkey and Bulgaria, and they are obtaining the rose oil almost exclusively from the single clone of R. damascena. In the recent studies a wide genetic diversity for R. damascena has been revealed in Iran, but there was a need to explore and evaluate the R. damascena in the neighboring areas of Iran. Thus the new research project was planned with the objective to evaluate the genetic diversity amongst R. damascena landraces grown in Pakistan and the subsequent comparison with tested germplasm grown in Iran. Further, the study is based on the collection of Damask and selected garden roses from Pakistan, Iran and USA, determining the characterization through microsatellite markers. The SSR markers confirmed the high level of diversity of the Rosa damascena germplasm within Iran and showed that the Pakistani genotypes were similar to those from the Iranian provinces of Isfahan, Kerman and Fars. The garden rose cultivars were distantly related.

Assessment of biodiversity based on morphological characteristics and RAPD markers among genotypes of wild rose species

Conservation and utilization of the native plant resources is essential for long term sustainability of biodiversity. Wild native resources are adapted to specific and diverse environmental conditions and therefore, these adaptive features can be introduced into modern cultivars either through conventional breeding or advanced molecular genetic techniques. Understanding the genetic make up of the wildly growing plant species and of target desirable genes is a prerequisite for this purpose. Five wild rose ( Rosa L.) genotypes were collected from different locations in northern hilly areas of Pakistan for this study. Different morphological characteristics and PCR based random amplified polymorphic DNA (RAPD) technique was used to find out the diversity and relationship among the genotypes. On morphological basis, Rosa webbiana collected from Muree and Nathia gali showed maximum (83%) similarity, whereas on DNA pattern basis, Rosa brunonii collected from Bansra gali and Sunny bank showed maximum (72%) similarity, while R. webbiana showed maximum diversity among all the species.

Evaluation of the Suitability of Sewage and Recycled Water for Irrigation of Ornamental Plants

The present study was conducted to evaluate the potential benefits and risks of the sewage water and recycled treated water on three ornamental plant species, including Umbrella plant (Cyperus alternifolius), Euonymus (Euonymus japonicas), and Dracaena (Cordyline terminalis). Plants in the pots were irrigated with equal volumes of water in 2-day intervals. Treatment plants were analyzed for sodium (Na), potassium (K), copper (Cu), iron (Fe), and lead (Pb) concentrations, in roots and shoots. The experiment was conducted by using a completely randomized design (CRD) with five replications. Data were analyzed using analysis of variance technique and least significance difference (LSD) test was applied at 5% probability level. Plants species performed better in sewage water after recycled water, in terms of growth, number of leaves, chlorophyll content, and leaf thickness, which proved sewage water accelerates the growth and development of ornamental foliage plants, but plants irrigated with this water exhibited greater concentration of Pb than other treatments.

Pollen Morphology and Viability Relates to Seed Production in Hybrid Roses

ABSTRACT Fertility of hybrid tea roses is often reduced due to their interspesific origin but also to intensive inbreeding. New genotypes used as pollen donors represent an economic risk for a breeding program, as their influence on seed production is unknown. In this study 9 garden rose genotypes were selected from a company database as high fertile or low fertile male parents, according to the number of seeds per hybridization. Pollen morphology and in vitro germination of the selected genotypes were characterized. Pollen was either small (mean diameter = 30 urn), shrunken, and irregular (abnormal), or large (mean diameter = 30 urn), elliptical and crossed by furrows (normal). High correlations were found between the number of seeds produced per hybridization and the pollen diameter (r = 0.94) or the percentage of normal pollen (r = 0.96). In order to evaluate the predictive power of the models, we conducted regression analyses and performed a validation experiment on genotypes not present in the database and without background information on fertility. Pollen diameter and percentage of normal pollen were characterized and fitted in the regression models for seed set predictions. Validation with an independent dataset gave a good prediction for 83.3% of the data. This indicates that using either the mean pollen diameter or the percentage of normal pollen resulted in effective fertility prediction. Moreover cluster analysis of the data classified all the cultivars into various groups with varying fertility. This tool could enhance the genetic variability in crossings between hybrid tea roses, thus creating possibilities for less economically risky exploitation of new tetraploid genotypes as male parents.

Heavy metal accumulation imparts structural differences in fragrant Rosa species irrigated with marginal quality water

Wastewater is an alternative to traditional sources of renewable irrigation water in agriculture, particularly in water-scarce regions. However, the possible risks due to heavy metals accumulation in plant tissues are often overlooked by producers. The present study aimed to identify heavy metals-induced structural modifications to roots of scented Rosa species that were irrigated with water of marginal quality. The chemical and mineral contents from the experimental irrigation canal water (control) and treated wastewater were below the limits recommended by the Pakistan Environmental Protection Agency (Pak-EPA) for medicinal plants. The experimentally untreated wastewater contained electrical conductivity (EC), chemical oxygen demand (COD), biological oxygen demand (BOD), and heavy metals (Co, Cu, Cd, Pb) that were above the recommended limits. The responses by wastewater-treated Rosa species (Rosa damascena, R. bourboniana, R. Gruss-an-Teplitz, and R. centifolia) were evaluated. The experimental data revealed that treated wastewater significantly increased the thickness of collenchyma (cortex and pith) and parenchyma tissues (vascular bundle, xylem, and phloem) of R. Gruss-an-Teplitz. Root dermal tissues (epidermis) of R. bourboniana also responded to treated wastewater. R. damascena and R. centifolia were the least affected species, under the experimental irrigation conditions. Collenchyma and dermal tissues were thicker in R. damascena and R. Gruss-an-Teplitz under untreated wastewater conditions. In parenchyma tissues, vascular bundles were thicker in R. damascena in untreated wastewater conditions, while the xylem and phloem of R. Gruss-an-Teplitz were thicker where treated wastewater was applied. In tissues other than the vascular bundle, the differences in anatomical metrics due to the experimental irrigation treatments were greater during the second year of the experiment than in the first year. The contents of metals other than chromium in the roots and stems of roses were below the WHO limits, under all of the experimental irrigation conditions. Rosa centifolia contained higher heavy metals content than the other experimental species, and heavy metals content was associated with anatomical changes due to the treatments. We conclude that, under conditions of wastewater irrigation, R. Gruss-an-Teplitz was highly resistant; R. damascena was moderately resistant while R. bourboniana and R. centifolia were the most susceptible to irrigation with marginal quality water. This is the first report of plant tissue responses to wastewater irrigation by the experimental species. Regarding the accumulation of heavy metals in rose plant tissues, the results confirm that untreated wastewater must be treated to grow Rosa species where water is scarce.

Evaluating sustainable and environment friendly substrates for quality production of potted Caladium

PurposeEffect of various combinations of potting substrates was assessed to enhance vegetative growth, plant productivity and mineral composition of Caladium bicolor.MethodsDifferent substrates like leaf compost, farmyard manure, coconut compost, compost and perlite were used in different combinations with silt. Bulbs were sown in pots filled with different combinations of potting substrates, which were arranged under completely randomized design (CRD), with three replications, and totally there were ten treatment combinations.ResultsApplication of different treatments of growing media either alone or in combinations led to considerable improvement in plant growth characteristics as compared to respective control. However, response differed according to the type of media manipulation. The highest stimulatory effect with maximum plant growth in terms of early sprouting, plant height, leaf area, chlorophyll contents, fresh and dry weights of tubers was observed in plants treated with silt+leaf compost+perlite combination; this combination significantly enhanced plant growth (30−150%) as compared to control. In case of NPK and protein contents, treatment combination of silt+leaf compost+coconut compost was greatly affected which lowered the pH, increased available organic matter and consequently maximized nutrient uptake by the plants.ConclusionsThe best selection of conventional organic and inorganic potting media is the key to successful mass propagation of containerized plants. Generally, it could be concluded that application of potting mixes with silt, perlite, leaf composts and coco-based residues is a good cultural practice to improve the crop productivity and provide a better growing environment for plants.

Physiological and Anatomical Response of Fragrant Rosa Species with Treated and Untreated Wastewater

The present study assessed the response of physiological and anatomical characteristics of four widely cultivated fragrant Rosa species. Water analysis showed that all minerals and chemicals were in permissible level in canal water and treated wastewater, whereas untreated wastewater contained higher EC, biological oxygen demand (BOD), chemical oxygen demand (COD) and heavy metals like Cd, Co, Cu, Pb. There was considerable variations among different Rosa species regarding response to wastewater irrigations. Under treated wastewater, R. bourboniana showed highest photosynthetic rate, high transpiration rate and maximum chlorophyll contents than other Rosa species whereas stomatal conductance of R. Gruss-anTeplitz was highest under treated wastewater. Leaf anatomical characteristics showed that R. Gruss-an-Teplitz under untreated wastewater showed large cortical cell area, vascular bundle area, large spongy cell area and thick midrib while large epidermal thickness of R. centifolia was recorded under treated wastewater. Large palisade cell and phloem area and thick leaves (lamina) were found in R. damascena under treated wastewater while large metaxylem area of R. bourboniana in untreated wastewater. The study showed that treated wastewater was most suitable and desirable irrigation treatment than canal water and untreated wastewater while R. bourboniana and R. Gruss-an-Teplitz was dominant Rosa species regarding physiological characteristics, while all species showed great diversity in leaf tissue architecture under treated and untreated wastewater.