Atsuhito Kuboki
Okayama University of Science
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Atsuhito Kuboki.
ACS Chemical Biology | 2012
Ken-ichiro Hayashi; Joshua Neve; Masakazu Hirose; Atsuhito Kuboki; Yukihisa Shimada; Stefan Kepinski; Hiroshi Nozaki
The plant hormone auxin is a master regulator of plant growth and development. By regulating rates of cell division and elongation and triggering specific patterning events, indole 3-acetic acid (IAA) regulates almost every aspect of plant development. The perception of auxin involves the formation of a ternary complex consisting of an F-box protein of the TIR1/AFB family of auxin receptors, the auxin molecule, and a member the Aux/IAA family of co-repressor proteins. In this study, we identified a potent auxin antagonist, α-(phenylethyl-2-oxo)-IAA, as a lead compound for TIR1/AFB receptors by in silico virtual screening. This molecule was used as the basis for the development of a more potent TIR1 antagonist, auxinole (α-[2,4-dimethylphenylethyl-2-oxo]-IAA), using a structure-based drug design approach. Auxinole binds TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and so inhibits auxin-responsive gene expression. Molecular docking analysis indicates that the phenyl ring in auxinole would strongly interact with Phe82 of TIR1, a residue that is crucial for Aux/IAA recognition. Consistent with this predicted mode of action, auxinole competitively inhibits various auxin responses in planta. Additionally, auxinole blocks auxin responses of the moss Physcomitrella patens, suggesting activity over a broad range of species. Our works not only substantiates the utility of chemical tools for plant biology but also demonstrates a new class of small molecule inhibitor of protein-protein interactions common to mechanisms of perception of other plant hormones, such as jasmonate, gibberellin, and abscisic acid.
Tetrahedron Letters | 2002
Susumu Ohira; Atsuhito Kuboki; Taisuke Hasegawa; Takato Kikuchi; Tatsuhiko Kutsukake; Maki Nomura
Abstract Sporochnols, fish deterrents from a marine alga, were synthesized, using intramolecular CH insertion of alkylidenecarbene as a key step to construct the chiral quaternary center.
Bioscience, Biotechnology, and Biochemistry | 2002
Susumu Ohira; Megumi Akiyama; Kumiko Kamihara; Yuichi Isoda; Atsuhito Kuboki
(1S,3R)-1-Aminocyclopentane-1,3-dicarboxylic acid (ACPD), a potent agonist of metabotropic glutamate receptors, was synthesized from L-serine. The chiral quaternary center was constructed by C–H insertion of the alkylidenecarbene, this being generated by the reaction between lithiotrimethylsilyldiazomethane and the corresponding ketone.
Tetrahedron Letters | 2007
Atsuhito Kuboki; Toru Yamamoto; Mamie Taira; Tetsuya Arishige; Susumu Ohira
Tetrahedron Letters | 2005
Megumi Akiyama; Yuichi Isoda; Masato Nishimoto; Aki Kobayashi; Daisuke Togawa; Nobuaki Hirao; Atsuhito Kuboki; Susumu Ohira
Tetrahedron Letters | 2004
Atsushi Yamazoe; Ken-ichiro Hayashi; Atsuhito Kuboki; Susumu Ohira; Hiroshi Nozaki
Tetrahedron Letters | 2004
Megumi Akiyama; Toshiki Awamura; Kazuhito Kimura; Yoshimi Hosomi; Ayako Kobayashi; Kazutaka Tsuji; Atsuhito Kuboki; Susumu Ohira
Tetrahedron Letters | 2004
Atsuhito Kuboki; Toshihiro Tajimi; Yoshihide Tokuda; Dai-ichiro Kato; Takeshi Sugai; Susumu Ohira
Tetrahedron Letters | 2013
Susumu Ohira; Kyouhei Takaya; Taichi Mitsui; Masahiro Kido; Kazuyuki Kakumoto; Ken-ichiro Hayashi; Atsuhito Kuboki; Hiroyuki Tani; Shogo Ikeda; Munekazu Iinuma; Yukihiro Akao; Hiroshi Nozaki
Tetrahedron Letters | 2008
Atsuhito Kuboki; Chie Maeda; Tetsuya Arishige; Kohei Kuyama; Mami Hamabata; Susumu Ohira