Attar S. Chawla

In-vivo degradation of poly(lactic acid) of different molecular weights

Lactic acid was polymerized using tetraphenyl tin as a catalyst. The molecular weight of the resultant poly(lactic acid) varied between 0.89 X 10(6) and 2.94 X 10(6) depending upon the concentration of the catalyst used. In-vivo degradation of the poly(lactic acid) samples having 4 different molecular weights were studies by implanting these in Wistar rats. It was found that at the end of a 48-week implantation period lower molecular weight poly(lactic acid) samples were degraded at a faster rate than the higher molecular weight samples.

Evaluation of plasma polymerized hexamethylcyclotrisiloxane biomaterials towards adhesion of canine platelets and leucocytes

Silicone coated Celgard-2400 and Silastic membranes were prepared by plasma polymerization of hexamethylcyclotrisiloxane. The adhesion of canine platelets and leucocytes was tested by passing whole blood from the anaesthetized mongrel dog in an ex-vivo shunt system. The silicone control Celgard and silicone coated Silastic membranes had fewer platelets and fewer leucocytes compared to those on the control Silastic membranes. Furthermore, these blood cells underwent fewer morphological changes on the silicone coated Celgard and Silastic compared to those on the control Silastic. From these observations the silicone coated biomaterials were judged to be better than the Silastic as far as the adhesion of platelets and leucocytes are concerned.

Albuminated Polymer Surfaces for Biomedical Application

Amino groups were added on to the surfaces of Celgard-2400 membranes by exposing them to an ammonia plasma. The presence of amino groups on the surfaces was detected by an attenuated total reflection Fourier Transform infrared spectrometer and by the Auger electron spectrometer. Through these amino groups, albumin was attached to the membranes. In some experiments, the attached albumin was further stabilized by cross-linking with glutaraldehyde. The effect of washing the albuminated membranes with saline and with plasma was investigated. It was observed that after the initial wash-out of albumin, the concentration of attached albumin tends to level off. The amount of albumin retained on the membranes varied between 275 to 357 micrograms/cm2.

Nonthrombogenic Surface by Radiation Grafting of Heparin: Preparation, in-vitro and in-vivo Studies

This is a study of the feasibility of radiation grafting of heparin to cellulose membranes. No significant amount of heparin could be bound to cellulose nitrate or cellulose acetate membranes by gamma radiation. Two-hour irradiated DEAECA membranes retained the highest amount of heparin; however, there was a 25% decrease in tensile strength. In-vitro studies show that blood did not clot even after 60 min on this membrane. In-vivo studies in dogs showed a significantly higher thrombo-resistance on the heparanized samples as compared to control samples.

Characterization of plasma polymerized polypropylene coatings.

Propylene was polymerized at low pressure in a radio frequency plasma reactor. The plasma polymerized propylene (PPP) films were insoluble in toluene. Thermal analysis of PPP showed no phase transition took place up to 255 degrees C. From these results, it was concluded that PPP is a highly crosslinked polymer. To further characterize the deposited PPP, Fourier Transform infrared (FTIR) spectroscopy in the attenuated total reflection (ATR) mode was used. The deposited material was shown to be polymerized propylene as a spectrum of the material showed absorption bands characteristic of polypropylene.

In vivo interactions between novel filler free silicone rubber and blood.

To evaluate the thrombogenicity of the novel filler free silicone rubber (FFSR) prepared in our laboratory, its interactions with blood were studied. The test samples (FFSR) along with control Silastic samples were placed in an extracorporeal shunt and primed with sterilized normal saline. The blood from an anaesthetized and systemically heparinized dog was passed over these samples for predetermined time intervals. After washing, fixing, etc., the platelets adhering to the samples were counted under light microscopy. To study the morphological changes of the platelets adhering to the samples, they were examined under scanning electron microscope. The number of platelets adhering to the FFSR samples was very small compared to the Silastic samples. For example, when the blood was passed over the samples for 10 min the number of platelets sticking per 0.14 mm2 surface area was 10 for FFSR samples compared to 380 for Silastic. Furthermore, there were no leukocytes on FFSR samples compared to 10 on Silastic. From these studies, the novel filler free silicone rubber prepared in our laboratory was judged to be more compatible with formed elements of blood compared to the Silastic material.

Effect of Gamma Radiation on the Molecular Weight and the Anticoagulant Activity of Heparin

4 g% aqueous solutions of heparin were irradiated with the gamma radiation doses of 4.6 x 10(5) or 9.2 x 10(5) rads. The irradiated and also the non-irradiated heparin samples were fractionated using a Sephadex G-200 column. With radiation, the peak of the molecular weight distribution curves shifted toward the lower molecular weight. Also, the number average molecular weight decreased by 8.2 and 11.5% with the doses of 4.6 x 10(5) and 9.2 x 10(5) rads, respectively. The anticoagulant activity depended on the molecular weight of the heparin fractions. For the heparin fractions with molecular weights below 7,900, the anticoagulant activity decreased with radiation. Thus, for a heparin fraction with a molecular weight of 6,200, the anticoagulant activity decreased from 211 to 198 IU/mg after 4 h of irradiation.