Aydin Erenmemisoglu

Effect of Some Antidepressants on Glycaemia and Insulin Levels of Normoglycaemic and Alloxan-induced Hyperglycaemic Mice

Depression is an important problem among diabetic patients. We have investigated the effect of some antidepressant drugs on plasma glucose and insulin levels in normoglycaemic and alloxan‐induced diabetic mice. For this purpose the effects of nortryptiline (as an example of a tricyclic antidepressant) and fluoxetine and sertraline (as examples of selective 5‐HT re‐uptake inhibitors) were examined on plasma glucose and insulin levels.

Hypoglycaemic Effect of Zizyphus jujuba Leaves

Zizyphus jujuba leaves have been widely used as a hypoglycaemic agent by diabetics in some regions of Turkey. In this study, the effects of Z. jujuba leaves on plasma glucose levels in normo‐ and hyperglycaemic rats were investigated. In addition, the chronic toxicity of Z. jujuba leaves was investigated in normoglycaemic rats.

Sister-chromatid exchange inducing effect of smokeless tobacco using on T-lymphocyte chromosomes

A kind of a smokeless tobacco (Maras powder) is widely used instead of cigarettes in the South Eastern region of Turkey. In this study we investigated the sister-chromatid exchange (SCE) inducing effect of this powder on the chromosomes of its users compared with smokers and nonsmokers using standard cell culture methods and SCE staining techniques. Average SCE per metaphase and total SCEs increased significantly among both smokeless tobacco users and smokers compared to nonsmokers (p < 0.01). However, the effect is significantly lower in smokeless tobacco users than in smokers (p < 0.05).

Bioequivalence Study of Two Different Clopidogrel Bisulfate Film-coated Tablets

OBJECTIVE The aim of the present study was to evaluate the bioequivalence of two oral clopidogrel (CAS 113665-84-2) formulations. METHOD The study was conducted as a monocentric, open, randomized, single-dose, two-period crossover trial in 48 healthy volunteers with a duration of hospitalization of approximately 24 h after dosing on day 1 and with a real wash-out period of 7 days. Blood samples were collected for 24 h post dosing in each period. The plasma was separated and the concentrations of clopidogrel were determined by a LC-MS/MS method. AUC(0-tlast), Cmax, tmax, AUC(0-inf), MRT and t1/2 were calculated for both formulations. RESULTS The arithmetic means of AUC(0-tlast) and Cmax were 3,656.01 pg x h/ml and 1970.22 pg/ml for the test formulation and 3771.51 pg x h/ml and 1756.52 pg/ml, respectively, for the reference formulation. The mean tmax was 1.16 h for the test and 1.13 h for the reference formulation. The point estimators of the ratios of the test and reference formulations for AUC(0-tlast) and Cmax were 1.042 and 1.115, respectively. Furthermore, the 90% confidence intervals calculated by means of ANOVA-log for the first primary endpoint of the trial, the intra-individual ratio (T/R) of AUC(0-tlast) of clopidogrel was between 0.932 and 1.165. The 90% confidence interval calculated by means of ANOVA-log for Cmax of clopidogrel was between 0.973 and 1.277. The 90% confidence intervals for both parameters were within the predefined acceptance ranges (0.80-1.25 for AUC(0-tlast) and 0.75-1.33 for Cmax). The intraindividual coefficients of variation determined by means of ANOVA-log were 33.51% for AUC(0-tlast) and 41.29% for Cmax. CONCLUSION While both products were bioequivalent in terms of the rate and extent of absorption, the present study also confirmed a high variability for clopidogrel suggesting high volunteer numbers in bioequivalence trials.

Do Non-Steroidal Anti-Inflammatory Drugs Induce Sister Chromatid Exchanges in T Lymphocytes?

The genetic toxicity of non-steroidal anti-inflammatory drugs was investigated using the sister chromatid exchange technique in cultured human lymphocytes. A total of 48 patients were treated with non-steroidal anti-inflammatory drugs (ibuprofen, ketoprofen, naproxen, indomethacin, diclofenac or acetylsalicylic acid) for 2 weeks. The average numbers of sister chromatid exchanges in cultured lymphocytes from the patients, before and after treatment with these drugs, did not differ significantly (P > 0.05). These results indicate that treatment with non-steroidal anti-inflammatory drugs for 2 weeks does not induce sister chromatid exchanges in T lymphocytes.

Proton pump inhibitors omeprazole, lansoprazole and pantoprazole induce relaxation in the rat lower oesophageal sphincter

Objectives  We aimed to investigate effects of the proton pump inhibitors (PPIs) omeprazole, lansoprazole and pantoprazole, which are currently used for the treatment of hyperacidity and gastro‐oesophageal reflux, on the reactivity of the isolated rat lower oesophageal sphincter.

ANTINOCICEPTIVE EFFECT OF PROPOFOL ON SOMATIC AND VISCERAL PAIN IN SUBHYPNOTIC DOSES

Abstract In this study we examined the effect of propofol on somatic and visceral pain in mice. A thermal method (tail immersion) and a chemical method (acetic-acid-induced writhing) were used to determine the antinociceptive effect of propofol. First we determined the dose-response relationships of propofol with a preliminary study. Then propofol was administered according to the results of the preliminary experiment. We used the subhypnotic and nonsedative doses of propofol in the experiments. This dose was lower than 10 mg/kg for mice according to our findings, and ED 50 sedation for propofol was 33 mg/kg. Propofol retarded tail withdrawal latencies and decreased writhing numbers of mice in a dose-dependent manner in dosages of 10 and 5 mg/kg ( P P P

Neuromuscular blocking effect of thiamphenicol on frog rectus abdominis muscle.

1. In the present study, thiamphenicol caused a significant fall in ACh-induced frog rectus abdominis muscle contraction peaks and significant rightward shifts of the contractile response curves in a dose-dependent manner. 2. In addition, the maximal ACh response of muscle could not be obtained in presence of thiamphenicol. On the other hand, thiamphenicol did not alter KCl-induced contractions of the muscle at the same concentrations. 3. In contrast chloramphenicol did not cause any significant change in ACh-induced contraction peaks. 4. These results have suggested that thiamphenicol may have a neuromuscular blocking effect on frog rectus abdominis muscle.