Aysun Camuzcuoglu

Increased prolidase activity and oxidative stress in PCOS.

Matrix metalloproteinases (MMPs) have been considered to have a role in various pathological processes, including inflammatory response, cardiovascular disease and recently also in ovarian dysfunction. Since prolidase could be accepted as a matrix metalloproteinase, on the biochemical level, we aimed to evaluate serum prolidase activity and oxidative–antioxidative status in patients with polycystic ovary syndrome (PCOS) and healthy individuals.

Decreased Serum Prolidase Activity and Increased Oxidative Stress in Early Pregnancy Loss

Aims: To compare the levels of serum prolidase activity and oxidative stress markers including total oxidative status (TOS), total antioxidant capacity (TAC), lipid hydroperoxide (LOOH) and total free sulfhydryl (–SH) in healthy pregnant women without early pregnancy loss (EPL) and women with EPL. Methods: Serum samples were obtained from 45 healthy first-trimester pregnancies and 45 pregnancies complicated with EPL. We have measured serum prolidase activity, TAC, TOS, –SH and LOOH levels spectrophotometrically. Results: Serum prolidase, TAC and –SH levels were significantly lower in the women with EPL than in the women without EPL (p <0.001, p = 0.001, p < 0.001, respectively), whereas TOS and LOOH levels were significantly higher (p < 0.001, p = 0.004, respectively). Prolidase activity was negatively correlated with TOS and LOOH levels (r = –0.299, p = 0.004; r = –0.323, p = 0.002, respectively), while positively correlated with TAC and –SH levels (r = 0.232, p = 0.028, and r = 0.418, p <0.001, respectively) Conclusion: Findings of this study have shown that serum prolidase activity and oxidative stress are significantly associated with the presence of EPL, and the correlation between serum prolidase activity and the markers of oxidative stress was reflected in increased serum TOS and LOOH levels and decreased serum TAC and –SH levels.

DNA damage and oxidative stress in patients with mild preeclampsia and offspring

OBJECTIVE Oxidative stress has been shown to play an important role in the pathogenesis of pre-eclampsia, and DNA damage frequently occurs in cells exposed to such stress. The aim of the present study was to investigate DNA damage and oxidative stress in mildly pre-eclamptic women and their offspring. STUDY DESIGN We studied 25 mildly pre-eclamptic mothers, 20 healthy controls, and their infants. Mononuclear leukocyte DNA damage, total antioxidant status (TAS), and total oxidant status (TOS) were determined and the oxidative stress index (OSI) was calculated. RESULTS DNA damage, and TOS and OSI levels were significantly increased, and TAS levels significantly decreased, in maternal and cord blood samples of the mildly pre-eclamptic group. A positive correlation between the extent of DNA damage and diastolic blood pressure was evident in pre-eclamptic mothers and there was a negative correlation between the extent of DNA damage and TOS. CONCLUSION Both oxidative stress and DNA damage are elevated in mildly pre-eclamptic patients and their offspring. Increased oxidative stress may be important in inducing DNA damage in pre-eclamptic patients.

May mean platelet volume levels be a predictor in the diagnosis of pelvic inflammatory disease

SummaryBackgroundOur aim in this study was to investigate whether mean platelet volume (MPV) value could be used as an early marker to predict pelvic inflammatory disease (PID).MethodsOverall, 44 patients with PID and 44 healthy women were included in the study. The control group consisted of 44 women who applied to the clinic for a routine gynaecological check-up, without chronic disease or a history of medication use. Owing to the fact that it would affect thrombocyte function, women who have the following conditions were excluded from the study: women who were taking anticoagulant therapy, oral contraceptives, nonsteroid anti-inflammatory medications and who had chronic diseases. The leukocyte count, platelet count, neutrophil ratio and MPV values were collected from PID and the control group. C reactive protein values of patients with PID were also noted.ResultsMPV values in patients with PID were lower than those in the control group. This reduction in MPV is statistically significant when the PID patient group is compared with the control group (p < 0.001). A negative correlation was discovered between platelet count and MPV values (p = 0.019, r = − 0.425). Receiver-operating curve analysis pointed out that MPV has greater area under curve value than neutrophil rate, leukocyte and platelet count (0.73, 0.64, 0.72 and 0.49 respectively).ConclusionSince the MPV value was significantly decreased in patients with PID, it may serve as an additional and even more valuable marker than leukocyte count in the diagnosis of PID.ZusammenfassungGrundlagenZiel dieser Studie war es zu untersuchen, ob das mittlere Thrombozytenvolumen (MPV) als früher Marker einer Erkennung von Entzündungen im Beckenbereich verwendet werden kann.Methodik44 Patienten mit Entzündungen im Becken und 44 gesunde Frauen wurden in die Studie aufgenommen. Die Kontrollgruppe waren Frauen, die die Ambulanz der Klinik zur gynäkologischen Routineuntersuchung aufgesucht hatten. Um andere Einflüsse auf die Thrombozytenfunktion zu vermeiden, wurden Frauen, die unter oraler Antikoagulationstherapie standen, die orale Kontrazeptiva und/oder nicht-steroidale antiinflammatorische Medikamente nahmen oder die eine chronische Erkrankung hatten, nicht in die Studie aufgenommen. Die Leukozytenzahl, die Thrombozytenzahl, der Neutrophilen Quotient und die MPV Werte wurden bei beiden Gruppen erhoben. Bei der Patientengruppe wurden zusätzlich die CRP Werte gemessen.ErgebnisseDie MPV Werte bei den Patientinnen waren niedriger als in der Kontrollgruppe (p < 0,001). Es bestand eine negative Korrelation zwischen der Thrombozytenzahl und den MPV Werten (p = 0,019, r = − 0,425). ROC Analysen ergaben, dass die MPV eine größere Fläche unter der Kurve hatte als die Neutrophilen Rate, oder die Leukozyten- beziehungsweise die Thrombozytenzahl (0,73, 0,64, 0,72 und 0,49 respektive).SchlussfolgerungDa das MPV bei Patientinnen mit Entzündungen im Beckenbereich signifikant erniedrigt war, kann dieser Parameter als ein zusätzlicher und im Vergleich zur Leukozytenzahl sogar wertvollerer Marker bei der Diagnose von Entzündungen im Beckenbereich dienen.

Oxidative stress and prolidase activity in women with uterine fibroids

The aim of this study was to investigate oxidative stress markers and prolidase activity in serum and tissue samples of women with uterine fibroids, with further analysis on position and size. Lipid hydroperoxide, ceruloplasmin, catalase, arylesterase, free sulfhydryl group activity and prolidase activity levels were measured in fibroid tissue, myometrial tissue and serum of the same patients (n = 51), at the same time. Results show that ceruloplasmin, catalase, arylesterase, free sulfhydryl group and prolidase activities were higher in fibroid tissue than those in myometrial tissue (p = 0.003, 0.009, 0.004, 0.02, 0.008, respectively). Serum levels of catalase and prolidase were lower, and arylesterase and free sulfhydryl groups were higher in the fibroid group than those in the control group (p < 0.001 for all). Fibroid volume in submucosal subgroup of the fibroid group yield significant correlation with ceruloplasmin, catalase, arylesterase and prolidase activities (r = 0.84, p = 0.02; r = 0.93, p < 0.001; r = 0.63, p = 0.049 and r = 0.87, p = 0.01, respectively). Despite the lack of statistical significance, the highest levels of prolidase activity were found in fibroid samples, especially in submucosal ones. It is concluded that this study demonstrated increased antioxidative repair system in the fibroid tissue compared to the myometrium and serum of the same patients. Additionally, higher pathophysiological potential of the submucosal fibroids over intramural and subserosal fibroids were shown with the levels of oxidative stress markers and prolidase activity levels.

Does a risky outcome of antenatal screening test indicate oxidative stress

Abstract Objective: The study aimed to investigate a possible relationship between second trimester aneuploidiy screening results and oxidative stress in foetal amnion and maternal serum. Methods: Concerning the outcome of the second-trimester screening test, 50 pregnant women of high risk were included in the experimental group, whereas 50 pregnant women with normal scores who wished to proceed with the amniocentesis procedure due to advanced maternal age and counselling were included in the control group. The biochemical parameters of total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were measured in the amniotic fluid samples and maternal serum samples. Results: OSI in the maternal serum samples, as well as TAS and TOS in the amniotic fluid, was significantly higher in the control group compared to the experimental group (p < 0.001, p = 0.047, p = 0.005, respectively). There was no significant difference in the TAS and TOS in the maternal serum samples or the OSI in the amniotic fluid between the groups. Conclusions: The results indicate that the positivity of the screening test is not significantly correlated with oxidative stress, a factor regarded as a pathological mechanism in various diseases. Potential maternal anxiety could underlie the elevated oxidative stress in the control group.

Effects of sevoflurane and desflurane on oxidative stress during general anesthesia for elective cesarean section

SummaryBackgroundAnesthetic agents might considerably influence maternal-fetal oxidative stress and antioxidants during cesarean section (CS). The aim of this study was to investigate the effects of desflurane and sevoflurane on oxidative stress parameters both in mothers and newborns undergoing elective CS.Materials and methodsEighty ASA physical status I–II, term parturients undergoing elective CS under general anesthesia were randomized to desflurane (Group D) and sevoflurane (Group S) groups. Blood samples were collected from mothers before operation and postoperatively and umbilical artery samples were obtained at delivery. Total oxidant status (TOS), total antioxidant capacity (TAC) status, lipid hydroperoxide (LOOH), and free sulfhydryl (–SH) levels were measured and oxidative stress index was calculated. Secondary outcomes included maternal hemodynamics.ResultsPreoperative LOOH, TOS, OSI, TAC, and –SH levels were similar among groups. Postoperative maternal serum LOOH, TOS, and OSI levels were significantly increased in Group D compared to Group S (p = 0.003, p = 0.005, p = 0.04; respectively). Postoperative umbilical artery LOOH, TOS, OSI levels were also significantly increased in Group D compared to Group S (p = 0.04, p = 0.02, p = 0.01; respectively). Postoperative TOS (p = 0.001, < 0.001 respectively) and OSI (p = 0.003, < 0.001 respectively) levels in both Group D and Group S were statistically significantly decreased compared to preoperative levels. Postoperative LOOH and –SH levels in Group S (p = 0.04, 0.029 respectively) were statistically significantly decreased compared to preoperative levels. There were no significant differences in TAC and –SH levels among groups (p = nonsignificant [n.s.]). Maternal perioperative mean blood pressure and heart rate were similar among groups (p = n.s.).ConclusionOxidative stress indices might be modified with preferred anesthetic agent and sevoflurane showed more favorable effects than desflurane in view of oxidative stress.ZusammenfassungHintergrundAnästhetika könnten den materno-fötalen oxidativen Stress und Antioxidantien während einer Sectio Caesarea (CS) erheblich beeinflussen. Ziel der vorliegenden Studie war es, die Wirkungen von Desfluran und Sevofluran auf Parameter des oxidativen Stresses sowohl bei Müttern, die sich einer elektiven CS unterziehen, als auch bei deren Neugeborenen zu untersuchen.Material und MethodenAchtzig zum Termin mittels elektiver CS unter Allgemeinnarkose Gebärende im ASA physikalischen Status I–II wurden randomisiert entweder Desfluran (Gruppe D) oder Sevofluran (Gruppe S) zugeteilt. Blutproben wurden von den Müttern prä- und postoperativ abgenommen, Nabelschnurblut bei der Geburt. Der Gesamt-Oxidations-Status (TOS), die totale antioxidative Kapazität (TAC), Lipid Hydroperoxid (LOOH) sowie die freien Sulfhydrylgruppen (–SH) wurden bestimmt. Der oxidative Stress Index (OSI) wurde berechnet. Die Hämodynamik der Mutter war ein sekundäres Outcome.ErgebnisseDie präoperativen LOOH, TOS, OSI, TAC und –SH Konzentrationen waren bei beiden Gruppen ähnlich. Die postoperativen mütterlichen LOOH, TOS, OSI Serum Konzentrationen waren in Gruppe D im Vergleich zur Gruppe S signifikant erhöht (p = 0,003, p = 0,005, p = 0,04; respektive). Postoperative Nabelschnur-Arterien Werte von LOOH, TOS, OSI waren in Gruppe D im Vergleich zur Gruppe S auch signifikant erhöht (p = 0,04, p = 0,02, p = 0,01; respektive). Postoperative TOS (p = 0,001, < 0,001 respektive) und OSI (p = 0,003,< 0,001 respektive) Werte waren sowohl in Gruppe D als auch Gruppe S statistisch signifikant im Vergleich zu den präoperativen Werten erniedrigt. Postoperative LOOH und –SH Konzentrationen waren in Gruppe S (p = 0,04, 0,029 respektive) im Vergleich zu präoperativen Werten statistisch signifikant erniedrigt. Auch die postoperative LOOH und –SH Werte der Gruppe S (p = 0,04, 0,029 respektive) waren im Vergleich zu den präoperativen Werten statistisch signifikant erniedrigt. Es bestand kein signifikanter Unterschied der TAC und –SH Werte zwischen den Gruppen (p = nicht signifikant [n.s.]). Auch der mittlere perioperative Blutdruck und die Herzfrequenz waren bei beiden Gruppen ähnlich (p = n.s).SchlussfolgerungenOxidative Stress Indikatoren können durch das bevorzugte Anästhetikum beeinflusst werden. Sevofluran zeigte bezüglich oxidativen Stress günstigere Wirkungen als Desfluran.

Oxigênio Suplementar em Cesariana Eletiva sob Raquianestesia: Manejar um Punhal com Cuidado

BACKGROUND AND OBJECTIVES We aimed to investigate the effect of 21% and 40% oxygen supplementation on maternal and neonatal oxidative stress in elective cesarean section (CS) under spinal anesthesia. METHODS Eighty term parturients undergoing elective CS under spinal anesthesia were enrolled in the study. We allocated patients randomly to breathe 21% (air group) or 40% (oxygen group) oxygen from the time of skin incision until the end of the operation. We collected maternal pre- and post-operative and umbilical artery (UA) blood samples. Total antioxidant capacity (TAC), total oxidant status (TOS) and the oxidative stress index (OSI) were measured. RESULTS Age, weight, height, parity, gestation week, spinal-skin incision time, skin incision-delivery time, delivery time, operation time, 1st and 5th minutes Apgar scores, and birth weight were similar between the groups (p > 0.05 for all comparisons). There were no differences in preoperative TAC, TOS, or OSI levels between the groups (p > 0.05 for all comparisons). Postoperative maternal TAC, TOS and OSI levels significantly increased in the oxygen group (p = 0.047, < 0.001 and 0.038, respectively); umbilical artery TAC levels significantly increased in the oxygen group (p = 0.003); and umbilical artery TOS and OSI levels significantly increased in the air group (p = 0.02 and < 0.001, respectively). CONCLUSIONS The difference in impact on maternal and fetal oxidative stress of supplemental 40% compared to 21% oxygen mandates further large-scale studies that investigate the role of oxygen supplementation during elective CS under spinal anesthesia.

Supplemental oxygen in elective cesarean section under spinal anesthesia: Handle the sword with care.

BACKGROUND AND OBJECTIVES We aimed to investigate the effect of 21% and 40% oxygen supplementation on maternal and neonatal oxidative stress in elective cesarean section (CS) under spinal anesthesia. METHODS Eighty term parturients undergoing elective CS under spinal anesthesia were enrolled in the study. We allocated patients randomly to breathe 21% (air group) or 40% (oxygen group) oxygen from the time of skin incision until the end of the operation. We collected maternal pre- and post-operative and umbilical artery (UA) blood samples. Total antioxidant capacity (TAC), total oxidant status (TOS) and the oxidative stress index (OSI) were measured. RESULTS Age, weight, height, parity, gestation week, spinal-skin incision time, skin incision-delivery time, delivery time, operation time, 1(st) and 5(th) minutes Apgar scores, and birth weight were similar between the groups (p > 0.05 for all comparisons). There were no differences in preoperative TAC, TOS, or OSI levels between the groups (p > 0.05 for all comparisons). Postoperative maternal TAC, TOS and OSI levels significantly increased in the oxygen group (p = 0.047, < 0.001 and 0.038, respectively); umbilical artery TAC levels significantly increased in the oxygen group (p = 0.003); and umbilical artery TOS and OSI levels significantly increased in the air group (p = 0.02 and < 0.001, respectively). CONCLUSIONS The difference in impact on maternal and fetal oxidative stress of supplemental 40% compared to 21% oxygen mandates further large-scale studies that investigate the role of oxygen supplementation during elective CS under spinal anesthesia.

Complete mole hydatidiform in a postmenopausal woman.

This absence of clear therapeutic guidelines reflects the fact that the poor prognosis risk factors are only partially understood. Accumulating evidence suggested that spontaneous regression of cancer most likely occurs by apoptosis of tumours cells rather than by other factors and the probable influences of genetic, environmental or auto-immune factors remain to be determined [2,7]. Our case suggests that, in young patients who wish to be pregnant, and conversely to malignant epithelial ovarian tumours, ‘‘debulking’’ surgery leaving a few implants in place can be of interest in order to limit surgical morbidity in cases with numerous digestive implants but also for the overall global prognosis of the illness. In these cases, a second look surgery can be performed to evaluate the need for and feasibility of a subsequent surgical treatment.