Azusa Saika
National Institute of Advanced Industrial Science and Technology
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Publication
Featured researches published by Azusa Saika.
Genome Announcements | 2014
Azusa Saika; Hideaki Koike; Tomoyuki Hori; Tokuma Fukuoka; Shun Sato; Hiroshi Habe; Dai Kitamoto; Tomotake Morita
ABSTRACT The basidiomycetous yeast Pseudozyma antarctica is known as a producer of industrial enzymes and the extracellular glycolipids, mannosylerythritol lipids. Here, we report the draft genome sequence of the type strain JCM10317. The draft genome assembly has a size of 18.1 Mb and a G+C content of 60.9%, and it consists of 197 scaffolds.
PLOS ONE | 2016
Azusa Saika; Hideaki Koike; Tokuma Fukuoka; Shuhei Yamamoto; Takahide Kishimoto; Tomotake Morita
Mannosylerythritol lipids (MELs) belong to the glycolipid biosurfactants and are produced by various fungi. The basidiomycetous yeast Pseudozyma tsukubaensis produces diastereomer type of MEL-B, which contains 4-O-β-D-mannopyranosyl-(2R,3S)-erythritol (R-form) as the sugar moiety. In this respect it differs from conventional type of MELs, which contain 4-O-β-D-mannopyranosyl-(2S,3R)-erythritol (S-form) as the sugar moiety. While the biosynthetic gene cluster for conventional type of MELs has been previously identified in Ustilago maydis and Pseudozyma antarctica, the genetic basis for MEL biosynthesis in P. tsukubaensis is unknown. Here, we identified a gene cluster involved in MEL biosynthesis in P. tsukubaensis. Among these genes, PtEMT1, which encodes erythritol/mannose transferase, had greater than 69% identity with homologs from strains in the genera Ustilago, Melanopsichium, Sporisorium and Pseudozyma. However, phylogenetic analysis placed PtEMT1p in a separate clade from the other proteins. To investigate the function of PtEMT1, we introduced the gene into a P. antarctica mutant strain, ΔPaEMT1, which lacks MEL biosynthesis ability owing to the deletion of PaEMT1. Using NMR spectroscopy, we identified the biosynthetic product as MEL-A with altered sugar conformation. These results indicate that PtEMT1p catalyzes the sugar conformation of MELs. This is the first report of a gene cluster for the biosynthesis of diastereomer type of MEL.
Genome Announcements | 2015
Tomohiko Matsuzawa; Hideaki Koike; Azusa Saika; Tokuma Fukuoka; Shun Sato; Hiroshi Habe; Dai Kitamoto; Tomotake Morita
ABSTRACT The yeast Starmerella bombicola NBRC10243 is an excellent producer of sophorolipids (SLs) from various feedstocks. Here, we report the draft genome sequence of S. bombicola NBRC10243. Analysis of the sequence may provide insight into the properties of this yeast that make it superior for use in the production of functional glycolipids and biomolecules, leading to the further development of S. bombicola NBRC10243 for industrial applications.
Applied Microbiology and Biotechnology | 2018
Azusa Saika; Hideaki Koike; Tokuma Fukuoka; Tomotake Morita
Mannosylerythritol lipids (MELs) are a type of glycolipid biosurfactant produced by basidiomycetous yeasts, most notably those belonging to the genera Pseudozyma and Ustilago. Mannosylerythritol lipids are environmentally friendly and possess many unique functions, such as gene delivery, bio-activation, and human skin repair, and thus have potential applications in cosmetic, pharmaceutical, agriculture, food, and environmental industries. However, MELs will require overcoming same issues related to the commercialization, e.g., expansion of the structure and function variety and cost reduction. In the past decade, various studies have attempted to tailor production of targeted MELs in order to expand the utility of these biosurfactants. Moreover, the rapid development of genomic sequencing techniques will enhance our ability to modify MEL producers. In this review, we focus on current research into the tailored production of MELs, including conventional and advanced approaches.
Genome Announcements | 2016
Yuka Sameshima-Yamashita; Hideaki Koike; Motoo Koitabashi; Azusa Saika; Tomotake Morita; Tohru Yarimizu; Hiroko Kitamoto
ABSTRACT Paraphoma sp. B47-9 is a producer of a biodegradable plastic–degrading enzyme. Here, we report the draft genome sequence of this strain. The draft genome assembly has a size of 39.3 Mb with a GC content of 52.4% and consists of 185 scaffolds.
Polymer Degradation and Stability | 2017
Shun Sato; Azusa Saika; Yukiko Shinozaki; Takashi Watanabe; Ken Suzuki; Yuka Sameshima-Yamashita; Tokuma Fukuoka; Hiroshi Habe; Tomotake Morita; Hiroko Kitamoto
Applied Microbiology and Biotechnology | 2018
Azusa Saika; Yu Utashima; Hideaki Koike; Shuhei Yamamoto; Takahide Kishimoto; Tokuma Fukuoka; Tomotake Morita
Journal of Bioscience and Bioengineering | 2017
Shoji Mizuno; Yukio Enda; Azusa Saika; Ayaka Hiroe; Takeharu Tsuge
Applied Microbiology and Biotechnology | 2017
Azusa Saika; Hideaki Koike; Shuhei Yamamoto; Takahide Kishimoto; Tomotake Morita
Journal of Bioscience and Bioengineering | 2018
Azusa Saika; Yu Utashima; Hideaki Koike; Shuhei Yamamoto; Takahide Kishimoto; Tokuma Fukuoka; Tomotake Morita
Collaboration
Dive into the Azusa Saika's collaboration.
National Institute of Advanced Industrial Science and Technology
View shared research outputsNational Institute of Advanced Industrial Science and Technology
View shared research outputsNational Institute of Advanced Industrial Science and Technology
View shared research outputsNational Institute of Advanced Industrial Science and Technology
View shared research outputsNational Institute of Advanced Industrial Science and Technology
View shared research outputsNational Institute of Advanced Industrial Science and Technology
View shared research outputs