B. Cappelli-Gotzos

Action de différents mélanges gazeux sur l'artère mésentérique de l'embryon de poulet explantée in vitro

The effect of various gas mixtures on the mesenteric artery of chicken embryo explanted in vitro has been studied. The expiants were cultivated in normal air, with addition of 3% of CO2, and in hypo- and hyperoxic atmosphere. The best survival of explanted arteries is obtained with atmospheric air. The hypoxic gas mixture (2% O2) has the most toxic effect on the expiants. The hyperoxic conditions show also a toxic effect, but in a lesser degree than hypoxic conditions.

In vitro responses of proliferating and non-proliferating cells to high doses of 17β-estradiol: Cytochemical study

The authors studied the effect of 17 beta-estradiol on the proliferation in vitro of chick embryo fibroblasts and human macrophages by microdensitometry, cytofluorometry, and autoradiography. For fibroblasts 17 beta-estradiol shortens the duration of the preparing period to mitosis, particularly of the synthetic phase (S) and has no effect on the duration of mitosis. For macrophages, which have temporarily lost their mitotic capacity, 17 beta-estradiol cannot induce mitotic division.

Peculiar Ultrastructural Features in the Cytoplasm of Cells from Human Effusions Associated with Malignant Disease

A peculiar structure revealed by the electron microscope in a few cells from two human pleural fluids, showing the morphologic features of metastasizing cancer cells in effusions, is described. It has the appearance of a rod-shaped pentalaminary structure approximately 25-35 nm thick formed by an outer double membrane, surrounding a central, more dense axis showing transverse striations at about 10-nm intervals. The double membrane often exhibits a terminal expansion connected with the endoplasmic reticulum and it is sometimes associated with microfilaments. These structures could be a variant of or represent a step in the formation of confronting cisternae, but the periodic striations they show in the more dense lamella give them some resemblance to a Langerhans cell granule. To date, such striations have not been seen in confronting cisternae.

Etude cytofluorométrique et histoautoradiographique de la synthèse d'ADN et de protéines sur des cellules mésothéliales humaines

The study of nuclear DNA synthesis of human mesothelial cells showed that cells do not divide by mitosis, even if an important percentage of them have a tetraploid amount of DNA. Concerning the cell cycle of mesothelial cells, the authors conclude that some of them are blocked in postsynthesis.

Sur une petite modification technique permettant une meilleure survie des artères de l'embryon de poulet explantées in vitro

The mesenteric artery of chicken embryo, cultivated in vitro in an abundant gas atmosphere (normal air) does not show the marked signs of cellular distress that appear when the artery is cultivated in the usual ‘salières’, containing a small quantity of oxygen.

Sur les relations entre la production d'hexosamines et d'hydroxyproline de la part de fibroblastes cultivés in vitro dans différentes concentrations d'O2

Studies and comparison of the hexosamine and hydroxyprolin production by fibroblasts cultivated in vitro at different oxygen concentrations. The results show that the highest hexosamine production occurs at a low oxygen concentration (5%). The highest hydroxyprolin production occurs at 20% O2; this oxygen concentration is considered as an hyperoxic one for fibroblasts cultivated in vitro. Besides it has been ascertained that the highest sulphated MPS production occurs between 5 and 20% oxygen without any significant variation.

Histoautoradiographic demonstration of cytoplasmic DNA synthesis in the cells from human ascitic fluid cultivated in vitro

Cells of human ascitic fluid, cultivated in vitro, showed an activity of cytoplasmic DNA synthesis apparently non-concomitant with a nuclear DNA synthesis.