B. E. Madari

Lessons from the Terra Preta de Índios of the Amazon region for the utilisation of charcoal for soil amendment

Neste trabalho faz-se a divulgacao do potencial de carvoes e residuos orgânicos parcialmente carbonizados visando obter materiais que mimetizam a materia orgânica do solo das Terras Pretas de Indio da Amazonia, e que sirvam como condicionadores de solo e sequestrem carbono de forma recalcitrante e reativa. Pesquisas desenvolvidas por grupos brasileiros e estrangeiros tem contribuido para o entendimento do surgimento e utilizacao das Terras Pretas de Indio da Amazonia. Aqui sao divulgados resultados de estudos quimicos no sentido do desenvolvimento do conhecimento cientifico e tecnologico e de inovacao no aproveitamento de subprodutos orgânicos, principalmente de industrias de biocombustiveis, carvao vegetal metalurgico e outros, buscando imitar a excelente performance da chamada Terras Pretas de Indio da Amazonia.

Fracionamento químico da matéria orgânica e características de ácidos húmicos de solos com horizonte a antrópico da amazônia (Terra Preta)

Amazonian dark earth soils that have anthropogenic A horizon (Au) present high natural fertility usually attributed to their high organic matter content and to their higher reactivity. In this study humic substances were quantified and the humic acids (HA) extracted from the 0-20 cm layer of Amazonian dark earth soils (Terra Preta do Indio) under forest and agricultural use were characterized. Adjacent soils with no Au horizon were also investigated. The HA were characterized through the thermogravimetric analysis, elemental composition analysis and characterization of functional groups (total, carboxylic, and phenolic). The A horizon of the anthropogenic soils presented higher total carbon level compared to adjacent soils. Among the humic fractions, the humin was the most abundant. The humic acid fraction (HA) was the dominant among the alkaline soluble fractions. The humic acids of the anthropogenic soils, both under forest and cultivation, showed higher humification degree compared to the non-anthropogenic soils. The agricultural cultivation affected the humic acids stability and reactivity in the anthropogenic soils.

Soil fertility and upland rice yield after biochar application in the Cerrado

The objective of this work was to evaluate the effect of biochar made from Eucalyptus on soil fertility, and on the yield and development of upland rice. The experiment was performed during two years in a randomized block design with four replicates, in a sandy loam Dystric Plinthosol. Four doses of NPK 05-25-15, annually distributed in stripes (0, 100, 200 and 300 kg ha-1), and four doses of biochar (0, 8, 16 and 32 Mg ha-1), applied once in the first year - alone or with NPK - were evaluated. In the first year, biochar positively affected soil fertility [total organic carbon (TOC), Ca, P, Al, H+Al, and pH], at 0-10 cm soil depth, and it was the only factor with significant effect on yield. In the second year, the effect of biochar diminished or was overcome by the fertilizer. TOC moved down in the soil profile to the 0-20 cm depth, influencing K availability in this layer. In the second year, there was a significant interaction between biochar and the fertilizer on plant growth and biomass dry matter accumulation.

Evidence of limited carbon sequestration in soils under no-tillage systems in the Cerrado of Brazil

The Brazilian government aims at augmenting the area cropped under no-tillage (NT) from 32 to 40 million ha by 2020 as a means to mitigate CO2 emissions. We estimated soil carbon (C) sequestration under continuous NT systems in two municipalities in the Goiás state that are representative of the Cerrado. A chronosequence of NT fields of different age since conversion from conventional tillage (CT) was sampled in 2003 and 2011. Soil C levels of native Cerrado and pasture were measured for comparison. After about 11 to 14 years, soil C stocks under NT were highest and at the levels of those under natural Cerrado. Average annual rates of soil C sequestration estimated using the chronosequence approach were respectively 1.61 and 1.48 Mg C ha−1 yr−1 for the 2003 and 2011 sampling, and were higher than those observed using repeated sampling after eight years. The diachronic sampling revealed that the younger NT fields tended to show higher increases in soil C stocks than the older fields. Converting an extra 8 million ha of cropland from CT to NT represents an estimated soil C storage of about 8 Tg C yr−1 during 10 to 15 years.

Comparison of DNA extraction protocols for microbial communities from soil treated with biochar

Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercial DNA extraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit Miniprep™ (ZR kit), for extracting microbial genomic DNA from sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.

Methods of soil organic carbon determination in Brazilian savannah soils

Several methods exist for determining soil organic carbon, and each one has its own advantages and limitations. Consequently, a comparison of the experimental results obtained when these methods are employed is hampered, causing problems in the comparison of carbon stocks in soils. This study aimed at evaluating the analytical procedures used in the determination of carbon and their relationships with soil mineralogy and texture. Wet combustion methods, including Walkley-Black, Mebius and Colorimetric determination as well as dry combustion methods, such as Elemental and Gravimetric Analysis were used. Quantitative textural and mineralogical (kaolinite, goethite and gibbsite) analyses were also carried out. The wet digestion methods underestimated the concentration of organic carbon, while the gravimetric method overestimated. Soil mineralogy interfered with the determination of carbon, with emphasis on the gravimetric method that was greatly influenced by gibbsite.

Biochar: Agronomic and environmental potential in Brazilian savannah soils

A B ST R A C T Due to the high activity of microorganisms, the loss of soil organic matter is high in tropical regions. This loss becomes even greater if the soil is managed improperly or when there is no technology that leverages the permanence of the soil carbon by maintaining appropriate levels of organic matter, providing chemical, physical and biological soil improvements and contributing to reduce CO 2 emissions to the atmosphere. Due to its aromatic structure, biochar is a highly stable form of carbon in the soil that may contribute to the reduction of greenhouse gas emissions, such as CO 2 , N 2 O and CH 4 , and act as a soil conditioner, improving the physical and chemical properties of the soil. Biochar may also result in increased productivity due to the improvement of soil attributes or a possible electrophysiological effect. Research over the past decades has demonstrated the potential of biochar as a soil conditioner, improving fertility and nutrient-use efficiency, in addition to maximising the productivity of crops, such as soybean and rice.

Produção e ciclagem de nutrientes por plantas de cobertura nas culturas de arroz de terras altas e de soja

The cover crops in no-till system can contribute to the formation of mulch and nutrient cycling to annual crops in succession. The objective of this study was to evaluate biomass production and nutrient cycling of cover crops sown in the second growing season, in crop rotation after upland rice and soybean, in no-tillage and conventional tillage systems, on a Red Latassol of Rio Verde, state of Goias, from April 2008 to April 2010. The experiment was evaluated in randomized strips, in a 5 x 6 factorial design, with four replications. In the horizontal strips two soil management systems (after three years of no-tillage and conventional systems) were evaluated and the cover crops in the vertical strips. Biomass and ground cover and nutrient cycling rates were only evaluated in the no-till treatments, in a 5 x 6 factorial arrangement, where the plots were subdivided, corresponding to six harvest dates of dried biomass 0, 15, 30, 60, 90 and 120 days after cutting of the cover crops. The following cover crops were sown in the second growing season: Brachiaria ruziziensis, Pennisetum glaucum and B. ruziziensis + Cajanus cajan and a fallow treatment as reference. Biomass production and the rates of soil cover and nutrient accumulation and release by cover crops as well as rice and soybean yield were evaluated. B. ruziziensis and B. ruziziensis + C. cajan performed best in biomass production, ground cover and nutrient accumulation at the end of the cover crops. The nutrients N and K had the highest concentration in the biomass, and the highest nutrient release to the soil was observed for K and P. The highest rice yield was observed when grown in no-tillage on crop residues of P. glaucum and B. ruziziensis, while soybean yields did not differ in the treatments.

Atributos biológicos do solo em pastagens de diferentes idades no sistema de integração lavoura‑pecuária

The objective of this work was to evaluate the effect of the pasture (Urochloa brizantha) component age on soil biological properties, in a crop-livestock integrated system. The experiment was carried out in a Brazilian savannah (Cerrado) area with 92 ha, divided into six pens of approximately 15 ha. Each pen represented a different stage of the pasture component: formation, P0; one year, P1; two years, P2; three years, P3; and final with 3.5 years, Pf. Samples were taken in the 0-10 cm soil depth. The soil biological parameters - microbial biomass carbon (MBC), microbial biomass respiration (C-CO2), metabolic quotient (qCO2), microbial quotient (qmic), and total organic carbon (TOC) - were evaluated and compared among different stages of the pasture, and between an adjacent area under native Cerrado and another area under degraded pasture (PCD). The MBC, qmic and TOC increased and qCO2 reduced under the different pasture stages. Compared to PCD, the pasture stages had higher MBC, qmic and TOC, and lower qCO2. The crop-livestock integrated system improved soil microbiological parameters and immobilized carbon in the soil in comparison to the degraded pasture.

Direct nitrous oxide (N2O) fluxes from soils under different land use in Brazil: a critical review.

Brazil typifies the land use changes happening in South America, where natural vegetation is continuously converted into agriculturally used lands, such as cattle pastures and croplands. Such changes in land use are always associated with changes in the soil nutrient cycles and result in altered greenhouse gas fluxes from the soil to the atmosphere. In this study, we analyzed literature values to extract patterns of direct nitrous oxide (N2O) emissions from soils of different ecosystems in Brazil. Fluxes from natural ecosystems exhibited a wide range: whereas median annual flux rates were highest in Amazonian and Atlantic rainforests (2.42 and 0.88 kg N ha-1), emissions from cerrado soils were close to zero. The decrease in emissions from pastures with increasing time after conversion was associated with pasture degradation. We found comparatively low N2O-N fluxes from croplands (-0.07 to 4.26 kg N ha-1 yr-1 , median 0.80 kg N ha-1 yr-1) and a low response to N fertilization. Contrary to the assumptions, soil parameters, such as pH, Corg, and clay content emerged as poor predictors for N2O fluxes. This could be a result of the formation of micro-aggregates, which strongly affect the hydraulic properties of the soil, and consequently define nitrification and denitrification potentials. Since data from croplands mainly derived from areas that had been under natural cerrado vegetation before, it could explain the low emissions under agriculture. Measurements must be more frequent and regionally spread in order to enable sound national estimates.