B.E. Seguin

Retained fetal membranes in cows: Manual removal versus nonremoval and its effect on reproductive performance

Sixteen primiparous Holstein cows with retained fetal membranes (RFM) were studied for postpartum prostaglandin release, uterine infection and resumption of estrous cyclicity after manual removal of RFM (eight cows) versus leaving the RFM untreated (eight cows). The RFM were results of induced parturition on Day 274 of gestation. Seventeen non-RFM primiparous cows were controls. The 15-keto-13, 14-dihydro-metabolite of prostaglandin F(2alpha) (PGFM) was measured in daily blood samples. Aerobic and anaerobic bacteria were cultured from weekly uterine swabs from Week 3 until results were negative. Resumption of estrous cyclicity was determined by milk progesterone three times weekly. Manual removal caused an immediate and large but short-lived increase in PGFM, probably due to the physical damage of uterine tissue. No sustained difference in postpartum PGFM release between cows with RFM manually removed and cows with RFM left untreated was detected. Non-RFM controls had lowest PGFM concentrations. Uterine infections were more frequent and more severe after manual removal of RFM. Untreated RFM-cows and controls were similarly affected. Most infections involved Actinomyces (formerly Corynebacterium ) pyogenes and/or Fusobacterium necrophorum . Actinomyces pyogenes was isolated in the third week postpartum in 5 8 cows with RFM manually removed versus 2 8 cows with RFM left intact and in 2 17 controls. Manual removal prolonged the interval from calving to first functional corpus luteum by 20 d. This study, using RFM resulting from induced parturition, shows that manual removal of RFM can delay the postpartum return to normal reproductive status without altering PGFM profiles.

Use of the prostaglandin F2α analog cloprostenol (ICI 80, 996) in dairy cattle with unobserved estrus

Abstract Two field trials were designed to evaluate use of the prostaglandin F 2α analog cloprostenol (CP) in dairy cattle with unobserved estrus and a palpably mature corpus luteum (CL). In Trial 1, 98 cows were treated with CP (500 μg IM) and 83 were given saline. Cows in both groups were assigned to be inseminated based on observed signs of estrus. Cows treated with CP were inseminated sooner than controls although heat detection still presented some difficulty. Conception rates were the same for both groups. In Trial 2, 154 cows were treated with CP for unobserved estrus and were assigned to be inseminated at estrus (78 cows) or were inseminated twice by appointment at 72 and 96 hours after treatment (76 cows). Appointment breeding eliminated problems associated with detection of estrus so nearly all cows were inseminated soon after treatment. In addition, cows bred twice by appointment had a higher conception rate than those bred based on signs of estrus. Induction of luteolysis with the prostaglandin F 2α analog CP in cows with unobserved estrus and a CL should prove to be a very effective addition to reproductive herd health programs. Successful use of CP for this purpose is dependent upon accurate diagnosis of the presence of a mature CL. In these trials 83 (92.4%) of 90 cows sampled had blood serum progesterone concentrations indicative (≥ 2.0 ng/ml) of significant luteal tissue activity.

Effect of stage of diestrus and number of cloprostenol (ICI 80, 996) injections on intervals to estrus, LH peak and ovulation in heifers.

Intervals to the onset of estrus, luteinizing hormone (LH) peak and ovulation were compared in diestrous heifers after each of two cloprostenol treatments. Diestrous heifers were grouped at the first treatment (T1) according to day of the cycle, with heifers on days 5 through 8 designated as early diestrus and heifers on days 9 through 17 designated as late diestrus. Cloprostenol treatment was repeated (T2) 11 days after T1, at which time heifers in both groups were at similar stages of the estrous cycle. Visual observation, identification of the preovulatory LH peak, and rectal palpation were utilized to estimate data parameters. Split-plot analysis of variance showed a significant treatment x group interaction (P < .05). Time from prostaglandin treatment to the onset of estrus was similar for the early diestrous group after T1 (x = 53.1 hours ) and the early and late diestrous groups after T2 (x = 49.4 hours and 45.4 hours respectively). This interval was longer (P < .05) for the late diestrous group after T1 (x = 60.8 hours ) than for either group after T2, but not different from that for the early diestrous group after T1. Serum progesterone concentrations were higher (P < .05) at the time of T1 in the late diestrous group (x = 5.8 ng/ml ) than in the early diestrous group (x = 3.0 ng/ml ) or in either group at the time of T2 (x = 2.8 and 3.2 ng/ml respectively). Over all heifers, the synchrony of the onset of estrus was more precise (P < .05) after T2 than T1. Intervals from the onset of estrus to ovulation were not affected by group or treatment (overall mean = 24.4 +/- 1.0 hours, n = 42). We conclude that different recommendations for appointment artificial insemination (AI) may be indicated depending on the number of prostaglandin injections which are used in a prostaglandin synchronization program prior to insemination.

Corpus luteum function and pregnancy rate in lactating dairy cows given human chorionic gonadotropin at middiestrus.

The effect of human chorionic gonadotropin (HCG) on corpus luteum (CL) function in lactating dairy cows and its subsequent effect on pregnancy rates achieved with artificial insemination was studied in two parts. In Part 1, four IM injections at intervals of seven days or two IM injections at a 14 day interval of HCG (10,000 IU) were given to two groups of six cows each, groups A and B respectively, beginning on days 9, 10 or 11 of the estrous cycle. Ten control cows were given 10 ml of isotonic saline solution on days 9, 10 or 11. Interestrous intervals were prolonged by an average of 8.1 and 3.4 days for groups A and B respectively over controls. In Part 2, 200 lactating dairy cows on nine farms were assigned, on an alternate basis at insemination, to control or treatment groups to study the effect of CL prolongation via HCG on pregnancy rate. A single injection of HCG (10,000 IU) was given 10, 11 or 12 days after a first or second insemination. Pregnancy rates for control and treated cows were similar (64 101 = 63% and 58 99 = 59% respectively). Interestrous intervals of treated nonpregnant cows were prolonged by approximately five days. Providing additional time for developing embryos to become established in utero by delaying luteolysis did not improve pregnancy rates achieved with artificial insemination in lactating dairy cows.

Simultaneous injection of PGF2α and GnRH into diestrous dairy cows delays return to estrus

Simultaneous injections of prostaglandin F2alpha (PGF) and gonadotropin releasing hormone (GnRH) or saline were given to 32 diestrous dairy cows to test the ability of GnRH to improve estrous and ovulation synchrony beyond that of PGF alone. Cows were randomly assigned to receive PGF on Day 8 or Day 10 of the estrous cycle (estrus = Day 0), and all cows were further assigned to simultaneous injection of GnRH or saline. Corpus luteum (CL) regression, return to estrus and follicular activity were monitored by plasma progesterone assay, twice-daily estrous detection and ultrasonographic examination, respectively. Plasma progesterone concentrations declined to <1.0 ng/ml at 24 hours after PGF in all cows and were not affected by GnRH. Gonadotropin releasing hormone inducted premature ovulation or delayed return to estrus in 7 of 8 cows treated with PGF/GnRH on Day 8 and 3 of 8 cows treated with PGF/GnRH on Day 10. Further, cows with premature GnRH-induced ovulations failed to develop and maintain a fully functional CL, and all returned to estrus 7 to 13 days after the induced ovulation. These data indicate that GnRH administered simultaneously with a luteolytic dose of PGF disrupts follicular dynamics and induces premature ovulation or delays normal return to estrus and, therefore, does not improve the synchrony of estrus and ovulation achieved with PGF alone.

Does intrauterine site of insemination in cattle really matter

Two trials were conducted to determine the influence of semen placement on pregnancy rate in dairy heifers and cows. Seventy-two dairy heifers were artificially inseminated (AI) 10 to 12 h after the first detection of estrus. Control heifers (n = 25) were inseminated at the junction of the uterine body and internal cervical os. The remaining heifers were inseminated deep in one uterine horn, 3 to 5 cm anterior to the external bifurcation. Twenty-three heifers were inseminated in the horn ipsilateral to the ovary bearing the ovulatory follicle, and 24 heifers were inseminated in the contralateral horn. Pregnancy rates did not differ for the three groups of heifers. In a second trial, 64 inseminations were performed in 38 nonlactating, adult dairy cattle. Thirty-one inseminations were made deep in the uterine horn ipsilateral to the ovary bearing the ovulatory follicle and 33 in the contralateral horn. Pregnancy rates were similar for both groups. Combining both trials, pregnancy rates for ipsilateral and contralateral inseminations were equal (32 54 = 59% and 34 57 = 60% , respectively). Therefore, placement of semen in one horn of the uterus does not appear to be a cause of decreased or increased pregnancy rate with AI.

Single appointment insemination for heifers after prostaglandin or progestin synchronization of estrus.

Pregnancy rates to a single appointment insemination were compared in seven groups of beef or dairy heifers following estrus synchronization with the prostaglandin F(2a) product, cloprostenol, versus the progestín product, Syncro-Mate-B. For cloprostenol synchronization, two injections of cloprostenol were given 11 d apart, with insemination occurring at 61+/-1 h after the second injection. The Syncro-Mate-B treatment consisted of a norgestomet/estradiol injection and a norgestomet implant on Day 0, followed by insemination at 49+/-1 h after implant removal on Day 9. Treatments were coordinated so all heifers in each group were housed together at the time of and for 48 h before a common insemination time so that the treatment received by individual heifers was not known. Overall pregnancy rates did not differ between treatment groups: 42% for 111 heifers treated with cloprostenol and 38% for 108 heifers treated with Syncro-Mate-B. Four blood samples per heifer taken during each replicate showed that two groups (n = 67) had many noncyclic heifers, while five groups (n = 152) had very few. Differences in pregnancy rates between these categories existed for both estrus synchronization methods, 18 versus 53% for cloprostenol and 21 versus 45% for Syncro-Mate-B. Thus the two methods were equally effective for cyclic heifers and equally ineffective for noncyclic heifers.

Ovulation rate after GnRH or PGF2α administration in early postpartum dairy cows

The objectives of this study evaluating induction of ovulation in early postpartum dairy cows were to: compare two methods of GnRH (100 mcg) administration (i.m. route and s.c. implant), and determine if prostaglandin F(2alpha) (PGF) causes release of LH or ovulation similar to that reported for GnRH. In trial #1, serum LH peaked at 2h after i.m. administration of GnRH and was declining at 4h. The s.c. GnRH implant also caused an elevation in serum LH at 2 and 4h after treatment, with LH declining at 6h. Serum LH was unchanged in control cows. Experimental treatment caused ovulation in 4 of 14 GnRH i.m. treated cows, 4 of 12 GnRH implanted cows and 0 of 13 control cows. Parity had no effect on LH response but did affect resulting ovulation rate as multiparous cows were more likely to ovulate than were primiparous cows in response to either GnRH treatment. All cows that ovulated had a follicle larger than 12 mm at the time of treatment. In trial #2, serum LH increased as before after i.m. administration of GnRH, however, serum LH was unchanged in cows treated with PGF or saline. Gonadotropin releasing hormone caused more cows to ovulate than did PGF or saline treatments, and GnRH shortened the interval from treatment to the onset of CL function over the PGF treatment; 13.9+/-2.6, 28.2+/-4.1 and 22.3+/-4.1 days for GnRH, PGF and saline, respectively. In summary, there was no difference in the ability of s.c. implantation and i.m. administration of GnRH to cause ovulation. Prostaglandin F(2alpha) did not cause release of LH or ovulation. In 22 early postpartum dairy cows treated with 100 mcg GnRH i.m. in these two trials, nearly all cows (95%) responded with a release of LH but only 45% (10/22) responded with an ovulation and subsequent formation of a CL.

Comparative luteolytic activity of estradiol cyclopentylpropionate and prostaglandin F2α in diestrous cows

Abstract The effect of estradiol cyclopentylpropionate (EC) on corpus luteum (CL) function in diestrous cows was evaluated. Two doses of EC (4 and 20 mg) were given by intramuscular (IM) injection and one dose of EC (4 mg) was given by intrauterine (IU) infusion. Control cows were treated with physiologic sterile saline (PSS) IU or corn oil IU (negative controls) or prostaglandin F 2α (PGF 2α , 30 mg IM, positive control). A total of 24 cows, four per treatment, were treated on days 8 to 12 of the estrous cycle (day 0 equals day of estrus). Luteal function was monitored by serum progesterone through 96 hours after treatment. A decrease in serum progesterone from pretreatment diestrous concentrations to less than 1.0 ng/ml was considered indicative of luteolysis. Intrauterine injection of PSS and corn oil had no effect on luteal function. Neither IM nor IU administration of EC caused consistent or rapid luteolytic effects. Prostaglandin F 2α consistently induced rapid luteal regression. These results indicate that EC should not be considered luteolytic in the same sense as is PGF 2α .

Interestrous intervals in cows after unilateral ovariectomy at estrus to prevent corpus luteum development

Abstract Two experiments were conducted to answer the question: What happens to the estrous cyclicity pattern of cows if ovulation and subsequent corpus luteum (CL) development are prevented by removing the ovary containing the largest follicle during estrus? In Experiment 1, the mean interestrous interval of the first estrous cycle after this procedure was 5.6 ± 0.6 days for 10 cows (one outlier of 2.5 d and one cow that ovulated from the remaining ovary were excluded) versus a 21.0 day mean for control cycles in the same cows. In Experiment 2, the same unilateral ovariectomy procedure was done but the effect of its timing was tested by performing the procedure before (n = 7 cows) or after (n = 5 cows) the anticipated luteinizing hormone (LH) surge at a prostaglandin-controlled estrus. Intervals to estrus (6.1 vs. 5.8 d) or to its LH surge (5.8 vs. 5.8 d) did not differ between these groups. Two cows treated before the LH surge that developed follicular cysts and one cow treated after the LH surge that ovulated from the remaining ovary were excluded. Therefore, the time required for cows to return to estrus from this controlled baseline point (unilateral ovariectomy at estrus of the ovary with the ovulatory follicle) was highly predictable (5.8 ± 0.6, n = 19), and the ovulatory mechanism, including magnitude of the LH surge, recovered in most cows despite the short interestrous interval.