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Dive into the research topics where B. G. Gasperin is active.

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Featured researches published by B. G. Gasperin.


Animal Reproduction Science | 2016

Metabolic and reproductive parameters in prepubertal gilts after omega-3 supplementation in the diet

Fabiana Moreira; Z.M.G. Cheuiche; G. Rizzoto; M.Q. Santos; M.S. Schuch; M.J. Flach; B. G. Gasperin; Ivan Bianchi; T. Lucia

Polyunsaturated fatty acids may benefit reproductive performance of female swine. This study evaluated metabolic and reproductive parameters of prepubertal finishing gilts fed with fish oil as a natural source of omega-3 fatty acids (6.88g/d) (n=12) over a period of 45 d. Gilts in the control group were fed soybean oil (n=13). Body weight and backfat were determined at 15-d intervals. Serum levels of leptin, IGF-1, insulin, cholesterol and triglycerides were measured at the beginning (D0) and at the end of the period (D45). Immunolabeling intensity for leptin and its receptor (ObRb) was assessed in oocytes of preantral follicles. Gilts fed omega-3 presented slightly heavier uteri (P=0.09) than control gilts, but there was no effect on body weight and backfat (P>0.05). Cholesterol serum levels tended to be lower at D45 for omega-3 supplemented gilts than for controls (P=0.06). Triglycerides and IGF-1 serum levels were lower at D45 than at D0 for control gilts (P<0.05), but unaltered for supplemented gilts. Insulin levels were unaffected by supplementation (P>0.05), but were greater at D45 than at D0 in both treatments (P<0.05). Immunolabeling for leptin and ObRb in oocytes included in preantral follicles was more intense for supplemented gilts than for control gilts (P<0.05). Omega-3 supplementation was associated with reduced serum cholesterol level and more intense staining for leptin in oocytes of prepubertal gilts, which suggests some involvement on triggering puberty.


Animal reproduction | 2016

Expression of growth and differentiation Factor 9 and cognate receptors during final follicular growth in cattle

C. S. Haas; Monique T. Rovani; Fernando Caetano de Oliveira; A. D. Vieira; Vilceu Bordignon; P. B. D. Gonçalves; Rogério Ferreira; B. G. Gasperin

Mutations in growth and differentiation factor 9 (GDF9) gene are associated to sterility or, paradoxically, increased ovulation rate in ewes. Despite its importance, the exact function of GDF9 in ovarian physiology is still poorly understood. This study aimed to investigate GDF9 function during dominant follicle growth and its regulation in follicular fluid. The regulation of GDF9 receptors in GnRH/LH-stimulated granulosa cells was also investigated. In a first experiment, a new follicular wave was induced and the intrafollicular GDF9 treatment into the largest growing follicle (8.5-9.5 mm) at both 100 (n = 3) and 1000ng/ml (n = 4) had no effect on follicular growth, estrus manifestation and ovulation compared to control (PBSinjected) follicles (n = 3). In a second experiment, follicles were obtained just after follicular deviation (day 4 after follicular emergence) and the abundance of GDF9 in follicular fluid did not differ between healthy dominant (n = 4) and atretic subordinate follicles (n = 4), as assessed by western blot analysis. Finally, mRNA expression of BMPR2 and TGFBR1 receptors was evaluated in granulosa cells obtained from preovulatory follicles (>12 mm diameter) obtained 0, 3, 6, 12 or 24 h after i.m. GnRH administration (n = 4-5 follicles/moment). Both receptors were significantly up regulated 12 h after GnRH treatment. Present results do not confirm the hypothesis that GDF9 inhibits dominant follicle growth and suggests a minor role in determining follicle fate. In the other hand, GDF9 receptors regulation in GnRH/LH-stimulated granulosa cells provides the first in vivo evidence of its involvement in the complex cascade of events that culminates in ovulation and luteinization in cattle.


Pesquisa Agropecuaria Brasileira | 2018

Motilidade de espermatozoides bovinos após incubação em fluído folicular

Sergio Farias Vargas Júnior; Vitória Gasperin Guazzelli Costa; Fernando Caetano de Oliveira; K. L. Goularte; C. S. Haas; B. G. Gasperin; Thomaz Lucia Júnior

The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total motility (TM), progressive motility (PM), curvilinear distance, and straightness, after 1 hour of incubation; and TM, PM, average path velocity, and curvilinear velocity after 3 hours of incubation. The ovarian follicle and follicular fluid do not provide a suitable environment to maintain bovine sperm cell motility.


Andrologia | 2018

Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls

C. E. R. Ferreira; C. S. Haas; K. L. Goularte; M. T. Rovani; F. F. Cardoso; Augusto Schneider; B. G. Gasperin; T. Lucia

The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT‐PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculates colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls.


Theriogenology | 2017

The acute effect of intravenous lipopolysaccharide injection on serum and intrafollicular HDL components and gene expression in granulosa cells of the bovine dominant follicle

Felipe Terres de Campos; Joao Aveiro Alvarado Rincon; Diego Andres Velasco Acosta; Pedro Augusto Silva Silveira; Jorgea Pradieé; Marcio Nunes Corrêa; B. G. Gasperin; Luiz Francisco Machado Pfeifer; Carlos C. Barros; Ligia Margareth Cantareli Pegoraro; Augusto Schneider


Reproduction in Domestic Animals | 2018

Natriuretic peptide system regulation in granulosa cells during follicle deviation and ovulation in cattle

Mp De Cesaro; Jt dos Santos; J. G. Ferst; J. E. Nóbrega; Pra da Rosa; Monique T. Rovani; Gustavo Freitas Ilha; Rodrigo C. Bohrer; Ronan Ferreira; B. G. Gasperin; Vilceu Bordignon; P. B. D. Gonçalves


Reproduction in Domestic Animals | 2016

Mechanistic target of rapamycin is activated in bovine granulosa cells after LH surge but is not essential for ovulation

Pra da Rosa; Amp Dau; Mp De Cesaro; Jt dos Santos; B. G. Gasperin; Raj Duggavathi; Vilceu Bordignon; P. B. D. Gonçalves


Animal reproduction | 2010

Role of angiotensin II on follicle development and ovulation

P. B. D. Gonçalves; V. M. Portela; Rogério Ferreira; B. G. Gasperin


Theriogenology | 2018

Estrus resynchronization in ewes with unknown pregnancy status

Vladinis O. Miranda; Fernando Caetano de Oliveira; Jenniffer H. Dias; Sergio Farias Vargas Júnior; K. L. Goularte; Manoel Francisco de Sá Filho; Ocilon G. de Sá Filho; Hernan Baldassarre; A. D. Vieira; T. Lucia; B. G. Gasperin


Livestock Science | 2018

Supplementation of diets with omega-3 fatty acids from microalgae: Effects on sow reproductive performance and metabolic parameters

C.J.M. Posser; L.M. Almeida; Fabiana Moreira; I. Bianchi; B. G. Gasperin; T. Lucia

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C. S. Haas

Universidade Federal de Pelotas

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P. B. D. Gonçalves

Universidade Federal de Santa Maria

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Monique T. Rovani

Universidade Federal de Santa Maria

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A. D. Vieira

Universidade Federal de Pelotas

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C. E. R. Ferreira

Universidade Federal de Pelotas

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K. L. Goularte

Universidade Federal de Pelotas

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T. Lucia

Universidade Federal de Pelotas

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Augusto Schneider

Universidade Federal de Pelotas

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