B. Sudhakara Reddy

Haemato-biochemical findings and thyroxin levels in canine demodicosis

In the present study, haemato-biochemical changes associated with canine demodicosis are reported. Dogs (n, 16) affected with generalized demodicosis revealed significant reduction in total erythrocyte count and haemoglobin levels. All the dogs had normal packed cell volume. Affected dogs also showed leukocytosis accompanied by neutrophilia, eosinophilia and lymphopenia. Dogs had normal serum protein levels, normal A/G ratio, reduced serum albumin levels, increased serum globulin and cholesterol levels. Dogs with demodicosis did not show any significant difference in total T4 and free T4 levels when compare with health dogs.

Efficacy of Cefpodoxime with Clavulanic Acid in the Treatment of Recurrent Pyoderma in Dogs

In the present study on recurrent pyoderma, dogs with a history of more than three episodes of skin infections in a period of one year were selected. The associated conditions and (or) underlying factors revealed upon thorough investigation were demodicosis, Malassezia dermatitis, flea infestation, hypothyroidism, keratinization disorder (seborrhea), combination of Malassezia dermatitis and tick infestation, and a combination of scabies and tick infestation. Therapy was given with cefpodoxime with clavulanic acid along with appropriate simultaneous medication for the underlying associated conditions. In all the cases response to therapy was excellent. Improvement was noticed by 9 to 19 days and 17 to 21 days in recurrent superficial and deep pyoderma, respectively. In one dog, relapse occurred by 45 days due to the associated condition of hypothyroidism which was confirmed through laboratory findings. Cefpodoxime with clavulanic acid proved to be an effective, safe, and convenient antibiotic for the treatment of recurrent pyoderma in dogs without any side effects.

Morphometry Of Demodex Canis And Demodex Cornei In Dogs With Demodicosis In India

In the two years period of research on canine dermatology at College Hospital of College of Veterinary Science Tirupati, 32 dogs with derma- tological problems found to have demodicosis. In these cases demodicosis was confirmed by clinical examination, microscopic examination of scrapings and tape impression smears collected from the lesions. In these two different types of Demodex mites were identified based on their habitat and morphology along with micrometry. Micrometry was carried out on 320 mites of two different types of Demodex collected from the all the cases. Demodex canis was identified in skin scrapings with pointed opisthosomal terminal end and mean body length of 211.81± 14.86 µm and mean width of 37.68±0.31 µm. Demodex cornei was noticed in tape impression smears and identification was based on its mor - phology (stubby farm with a blunt posterior opisthosoma end) and its mean length of 137.15 ± 37.72 µm and mean width of 38.28±0.19 µm. Lengths of total body and opisthosoma of both types of the mites differed statistically significantly but, gnathosoma and podosoma did not differ significantly.

Dermatitis due to Mixed Demodex and Sarcoptes Mites in Dogs

In dogs, dermatitis due to mixed mite infestation is rare. During the five-year period of study, two dogs were identified suffering from dermatitis due to mixed Demodex and Sarcoptes mites. Upon clinical examination dogs had primary and secondary skin lesions on face, around the ears, chin, neck, fore limbs and lateral abdomen. Microscopic examination of skin scrapings revealed Demodex and Sarcoptes mites. Both dogs were treated with daily oral ivermectin at 100 to 400 μg/kg body weight as incremental doses, external application of amitraz and supportive treatments with topical antimicrobial shampoo. After completion of forty-two days of therapy, dogs were recovered from the dermatitis.

EVALUATION OF THE EFFICACY OF POLYHERBAL ANTI-STRESSOR PRODUCT (RESTOBAL) AGAINST STRESS INDUCED BY Balantidium coli INFECTION IN BUFFALOES

The present study was conducted to determine the alterations in haematological parameters and stress markers in naturally Balantidium coli infected buffaloes, along with this efficacy of polyherbal antistressor product Restobal (Restobal ® , M/s Ayurvet Limited) was also evaluated against the stress induced by B. coli. In present study, a total of ten apparently healthy buffaloes were selected and considered as control group while twenty buffaloes with natural B. coli infection were included in the disease group and considered for treatment trial. In all the clinical cases, clinical examination and detailed appraisal of haemato-biochemical profiles were carried out. For treatment trial, a total of twenty diseased buffaloes were divided into two groups with ten buffaloes in each group. In therapeutic trials (group I) buffaloes were administered with specific medication against B. coli infection and in herbal trail buffaloes (group II) were administered with specific medication against B. coli infection along with * Corresponding author KEYWORDS

Trypanosoma evansi infection in a cat—a rare case

A seventeen-month-old tom cat was presented to the hospital with the history of pyrexia, unable to stand with forelimbs and abnormal mentation. Trypanosoma evansi organisms were detected in the stained peripheral blood smears collected from the cat. The cat had anemia, hypoglycemia and hypoalbuminemia. The cat was successfully treated with injection diminazene aceturate along with other supportive medication and uneventfully recovery was noticed by the sixth day in his activities.

CD4+ and CD8+ T cells in the peripheral blood of dogs affected with generalised demodicosis

Canine generalised demodicosis is recognised as a severe skin disease of dogs, and studies on CD4+ and CD8+ T cells in this disease condition have been limited. The aim of the present study was to record the CD4+ and CD8+ T cells in the peripheral blood of dogs affected with generalised demodicosis. Whole blood was collected from dogs with generalised demodicosis for estimation of CD4+ and CD8+ T cells by flow cytometry. The demodicosis-affected dogs had lower number of CD4+ T cells, increased CD8+ T cells, and reduced CD4+/CD8+ ratio compared to healthy controls. Present abnormalities in the study are related to the immunological response of the dogs to the mites in a disease process.

Therapeutic management of epistaxis due to ehrlichiosis in a dog

A Labrador bitch was diagnosed that it was suffering with clinical ehrlichiosis. Dog exhibited epistaxis, tachycardia and hyperpnoea. Dog showed signs of anaemia, increased alanine transaminase and aspartate transaminase levels. Present case was successfully treated with oxytetracycline and doxycycline along with supportive therapy.

Rapid serodiagnosis of Trypanosoma evansi in dogs by colloidal dye immunobinding assay

Trypanosoma evansi causes significant mortality in dogs. Diagnosis of T. evansi infection can be done by different diagnostic methods including wet blood film examination, stained blood smear examination, advanced serological and molecular tests. In the present study, colloidal dye immunobinding technique was developed for diagnosis of T. evansi in dogs. In this technique, the whole cell lysate antigen was coated on the nitrocellulose membrane of the flow-through device. Protein A colloidal gold was used as detector. The evaluation of the technique was performed by comparing the known positive T. evansi serum collected from dogs and known negative serum. The assay has been validated with wet blood film examination. The present test is an acceptable alternative for use in clinical laboratories which lacks the specialized equipment for screening of T. evansi infection in dogs.