Bagus Komang Satriyasa

The Number of Leydig Cells, Sertoli Cells, and Spermatogonia are Lower towards a Little Rats that Their Parent Given Genistein during Periconception Period

The testis was composed by the cells of Leydig, Sertoli, and Spermatogenic. The cells formation itself was able to be disrupted by exposure to an endocrine disrupting chemical (EDC) since the prenatal period. The research was intended to prove that Genistein could obstruct the formation of Leydig cells, Sertoli cells, Spermatogenic of rats. The randomized post-test only control group design method was conducted towards white female Wistar rats, 12-13 weeks old, has been had one child, could normally eat and drink. The analysis unit was the child of mother rat treatment group that given Genistein of 10 mg/kg/day and the control group that received distilled water, each of them were 15. The research was done in the Laboratory of Faculty of Veterinary Medical, Udayana University, from January to July 2016. The computer was used for analyzing the data using, with α of 0.05. The result was the Genistein child had an average of 5.464 Sertoli cell, 11.120 Leydig cell, and 48.427 spermatogonia, whereas, the control group had an average of 8.173 Leydig cells, 12.987 Sertoli cells, and 69.547 spermatogonia. There were significant differences between the two groups ( p 0.000). The conclusion was that an average of Leydig cells, Sertoli cells, and Spermatogonia lower in children whose their parent rats given Genistein during Periconception period.