Banu Bayram

Association of angiotensin converting enzyme (ACE) gene I/D polymorphism and polycystic ovary syndrome (PCOS).

This study was conducted in Turkish patients with polycystic ovary syndrome to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in polycystic ovary syndrome development. Genomic DNA obtained from 200 persons (100 patients with polycystic ovary syndrome and 100 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with a charge coupled device (CCD) camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (p<0.001). The D allele frequency was indicated as 68% and I allele was as 32% in the patients, whereas it was 51.5-48.5% respectively in the control group. As a result of our study we may assert that angiotensin converting enzyme gene I/D polymorphism DD genotype should be considered as a genetic marker in polycystic ovary syndrome development in this Turkish study population.

Role of phenolic compounds in nitric oxide synthase activity in colon and breast adenocarcinoma.

Cancer chemopreventive agents are designed to reduce the incidence of tumorigenesis by intervening at one or more stages of carcinogenesis. This study aimed to determine the effects of resveratrol (RES) and tannic acid (TA), which are chemopreventive agents, on the nitric oxide synthase (NOS) levels that are effective for development of cancer in colon and breast cancer cell lines. The CaCo-2 (human colon carcinoma cell line) and MCF-7 (Michigan Cancer Foundation-7; human breast adenocarcinoma cell line) cells were grown in the laboratory. RES and TA were used to treat CaCo-2 and MCF-7 cells. Nitric Oxide Synthase Assay Kit was used to determine the NOS enzyme activity of CaCo-2 and MCF-7. Statistical differences between control and RES- and TA-treated cells were calculated using the Students t-test for double comparison. It was observed that NO activity was generally decreased in CaCo-2 and MCF-7 cells, in which RES and TA were applied. Results suggest that the phenolic compounds RES and TA have different effects on NOS enzyme activity of the colon and breast cancer cells.

Plasminogen Activator Inhibitor Type-1 Gene 4G/5G Polymorphism Is Associated with Hypertensive Patients in the Turkish Population

This study has been performed on hypertensive patients in the Turkish population to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene and with the aim of examining the role of this polymorphism in hypertension development. Genomic DNA obtained from 284 persons (176 patients with hypertension and 108 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using 4G and 5G allele-specific primers. Polymerase chain reaction products were assessed by being exposed to 2% agarose gel electrophoresis. Results were evaluated with the chi-square test. The 4G allele frequency was 31.25% and the 5G allele frequency was 68.75% in patients, whereas it was 49/51% in a control group. 5G5G genotype was found statistically high (p < 0.001) in patients relative to controls. This study showed that the plasminogen activator inhibitor type-1 gene 4G/5G polymorphism and the 5G5G genotype appear to be associated with an elevated risk of developing hypertension in a representative sample of Turkish population.

Plasminogen activator inhibitor type-1 gene 4G/5G polymorphism and polycystic ovary syndrome.

AIM This study was conducted in Turkish patients with polycystic ovary syndrome (PCOS) to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene and to examine the role of this polymorphism in PCOS development. MATERIALS AND METHODS Genomic DNA obtained from 200 persons (100 patients with PCOS and 100 healthy controls) was used in the study. DNA was amplified by polymerase chain reaction using 4G and 5G allele-specific primers. Polymerase chain reaction products were assessed with charged-couple device camera after exposing to 2% agarose gel electrophoresis. RESULTS No statistically significant difference between the groups with respect to genotype distribution was found (p>0.05) in the study. The 4G and 5G allele frequencies were indicated as 51.5% and 48.5% in patients, respectively, whereas this was 51%-49% in the control group. CONCLUSION Consequently, it has been established by this study that the 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of PCOS in the Turkish population.

The Association Between Development and Progression of Multinodular Goiter and Thyroid-Stimulating Hormone Receptor Gene D727E and P52T Polymorphisms

AIM This study has been performed on a Turkish population with multinodular goiter (MNG) to investigate the thyroid-stimulating hormone receptor (TSHR) gene D727E and P52T polymorphisms. METHODS DNA samples were isolated from 300 patients with MNG and 142 controls. Polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis were used. RESULTS The D727E polymorphism G-allele frequency and the CG and GG genotypes were significantly higher in patients with MNG. However, there was no significant difference in the P52T polymorphism between patients and control subjects. CONCLUSIONS As a conclusion, the D727E polymorphism G allele may be related to MNG development in the studied population.