Baodi Dong

Effects of Irrigation and Straw Mulching on Microclimate Characteristics and Water Use Efficiency of Winter Wheat in North China

Abstract In North China, irrigation is required to obtain a high yield from winter wheat; this results in rapid aquifer depletion. The primary objective of this study was to investigate the influencing mechanisms of irrigation and straw mulching in preserving the soil moisture. Maize straw (3−5 cm) was mulched immediately after sowing winter wheat, and irrigation water was supplied at 60 mm, controlled by using a flow meter, during the jointing, heading, or milking stages of the crop. The results revealed that irrigation decreased the eddy thermal diffusivity, sensible heat flux, and soil heat flux, but increased the latent heat flux. In contrast, straw mulching enhanced the eddy thermal diffusivity and sensible heat flux, but decreased the latent heat flux. Straw mulching increased the soil temperature at 5 cm depth form January to February, but decreased the soil temperature before January and after February. There were no significant differences in the total evapotranspiration between mulched and non-mulched treatments, however, there was a statistically significant difference in the evapotranspiration among the different growing seasons. Straw mulching reduced the evapotranspiration from the seeding stage to the regrowing stage, and the evapotranspiration with mulching was less than that non-mulching 47.4 mm. Further, straw mulching significantly reduced the number of spikes in the crop. Both irrigation and straw mulching increased the number of kernels, but had no visible effects on the thousand kernel weight. These results indicate that straw mulching may decrease the yield and water use efficiency (WUE) of winter wheat in North China.

The Wheat GT Factor TaGT2L1D Negatively Regulates Drought Tolerance and Plant Development

GT factors are trihelix transcription factors that specifically regulate plant development and stress responses. Recently, several GT factors have been characterized in different plant species; however, little is known about the role of GT factors in wheat. Here, we show that TaGT2L1A, TaGT2L1B, and TaGT2L1D are highly homologous in hexaploid wheat, and are localized to wheat chromosomes 2A, 2B, and 2D, respectively. These TaGT2L1 genes encode proteins containing two SANT domains and one central helix. All three homologs were ubiquitously expressed during wheat development and were responsive to osmotic stress. Functional analyses demonstrated that TaGT2L1D acts as a transcriptional repressor; it was able to suppress the expression of AtSDD1 in Arabidopsis by binding directly to the GT3 box in its promoter that negatively regulates drought tolerance. TaGT2L1D overexpression markedly increased the number of stomata and reduced drought tolerance in gtl1-3 plants. Notably, ectopic expression of TaGT2L1D also affected floral organ development and overall plant growth. These results demonstrate that TaGT2L1 is an ortholog of AtGTL1, and that it plays an evolutionarily conserved role in drought resistance by fine tuning stomatal density in wheat. Our data also highlight the role of TaGT2L1 in plant growth and development.

Comparison of the effects of symmetric and asymmetric temperature elevation and CO2 enrichment on yield and evapotranspiration of winter wheat (Triticum aestivum L.).

Under the changing climate, asymmetric warming pattern would be more likely during day and night time, instead of symmetric one. Concurrently, the growth responses and water use of plants may be different compared with those estimated based on symmetric warming. In this work, it was compared with the effects of symmetric (ETs) and asymmetric (ETa) elevation of temperature alone, and in interaction with elevated carbon dioxide concentration (EC), on the grain yield (GY) and evapotranspiration in winter wheat (Triticum aestivum L.) based on pot experiment in the North China Plain (NCP). The experiment was carried out in six enclosed-top chambers with following climate treatments: (1) ambient temperature and ambient CO2 (CON), (2) ambient temperature and elevated CO2 (EC), (3) elevated temperature and ambient CO2 (ETs; ETa), and (4) elevated temperature and elevated CO2 (ECETs, ECETa). In symmetric warming, temperature was increased by 3°C and in asymmetric one by 3.5°C during night and 2.5°C during daytime, respectively. As a result, GY was in ETa and ETs 15.6 (P < 0.05) and 10.3% (P < 0.05) lower than that in CON. In ECETs and ECETa treatments, GY was 14.9 (P < 0.05) and 9.1% (P < 0.05) higher than that in CON. Opposite to GY, evapotranspiration was 7.8 (P < 0.05) and 17.9% (P < 0.05) higher in ETa and ETs treatments and 7.2 (P < 0.05) and 2.1% (P > 0.05) lower in ECETs and ECETa treatments compared with CON. Thus, GY of wheat could be expected to increase under the changing climate with concurrent elevation of CO2 and temperature as a result of increased WUE under the elevated CO2. However, the gain would be lower under ETa than that estimated based on ETs due to higher evapotranspiration.

Effects of Drought Stress on Pollen Sterility, Grain Yield, Abscisic Acid and Protective Enzymes in Two Winter Wheat Cultivars

Drought stress induced pollen sterility is a detrimental factor reducing grain number in wheat. Exploring the mechanisms underlying pollen fertility under drought conditions could assist breeding high-yielding wheat cultivars with stress tolerance. Here, by using two Chinese wheat cultivars subjected to different levels of polyethylene glycol (PEG)-induced drought stress, possible links between pollen fertility and stress tolerance were analyzed under different levels of drought stress at the young microspore stage. In both cultivars, higher grain number reduction was observed under condition of lower water availability. Overall, the drought tolerant cultivar (Jinmai47) exhibited less grain number reduction than the drought sensitive cultivar (Shiluan02-1) under all stress conditions. Compared with Shiluan02-1, Jinmai47 exhibited superior physiological performance in terms of leaf photosynthetic rate, ear carbohydrate accumulation, pollen sink strength, pollen development and fertility under stress. Moreover, Jinmai47 showed a lower increase in endogenous abscisic acid in ears than Shiluan02-1. Furthermore, higher levels of superoxide dismutase (SOD) and peroxidase (POD) activities were also found in the drought tolerant cultivar Jinmai47 under PEG stress, compared with the drought sensitive cultivar Shiluan02-1. Changes in these physiological traits could contribute to better pollen development and male fertility, ultimately leading to the maintenance of grain number under drought stress.