Barbara H. Fadem

Dose-response and time-response relationships between progesterone and the display of patterns of receptive and proceptive behavior in the female rat

Abstract The relationship between administration of progesterone and the display of patterns of receptive (response to the male) and preceptive (female initiated) sexual behavior was examined in ovariectomized, estrogen-primed female rats in a “restrained male” test situation. It was found that the degree of receptivity and proceptivity displayed was directly proportional to progesterone dose and time from progesterone injection (up to 4.5 hr). Higher progesterone doses and longer period of time from progesterone injection (up to 4.5 hr) were both associated with shorter latencies to return to the male following intromission and ejaculation. Receptivity could be induced with estrogen alone but progesterone was required for the display of proceptivity and higher doses of progesterone were needed to effect increases in proceptivity relative to receptivity. Proceptive behavior also occurred in a narrower time range than did receptive behavior. Receptivity alone is characterized as the lowest degree, and receptivity plus proceptivity as the highest degree, of expression of the total behavior pattern of the estrous female rat. Receptivity and proceptivity together constitute a continuum of estrous responsiveness. Increasing the progesterone dose from 0 to 200 μg, and increasing the latency from progesterone injection from 0 to 4.5 hr, were associated with increasing degree of expression of the total behavioral continuum.

Sexual Behavior of the Gray Short-Tailed Opossum (Monodelphis domestica)

Sexual behavior was studied in captive specimens of the gray short-tailed opossum ( Monodelphis domestica ), a small, Brazilian, didelphid marsupial. A “switch-male” procedure in which males are alternated in breeding pairs was devised to encourage mating. Nonreceptive females are strongly aggressive toward males. Male courtship behavior includes strategies aimed at reducing this aggressive response. In copulation, the male clasps the females ankles with his hind feet and the pair usually lies upon the right side. Copulation involves intravaginal thrusting, locking, and apparently a single intromission and ejaculation. These behavior patterns are compared with those of other commonly studied didelphids. Since M. domestica will breed in the laboratory, it may prove to be a practical marsupial model for studies of early mammalian development.

Neonatal hormonal influences on the development of proceptive and receptive feminine sexual behavior in rats

Abstract The objective of this study was to examine the influence of androgen and of the inhibiting of aromatization of androgen to estrogen during the early neonatal period on the development of receptive (lordosis and acceptance of stimulus male mounting attempts) and proceptive (affiliation with and solicitation of stimulus males) feminine sexual behavior. Within 8 hr of birth, male rats were castrated or received subcutaneous implants of the aromatase inhibitor androst-1,4,6-triene-3, 17-dione (ATD) while females received injections of testosterone propionate (TP). At 90 days of age all treated animals and controls were tested for receptive and proceptive feminine sexual behavior. It was found that androgen present neonatally blocked proceptive as well as receptive behavior patterns in adult rats. The proceptive and receptive feminine sexual behavior patterns displayed by adult males deprived of the effects of androgen neonatally either by castration or by treatment with ATD were comparable to those of normal females.

Nest-building in gray short-tailed opossums: temperature effects and sex differences.

The effects of ambient temperature and of sex on nest-building behavior were studied in a laboratory colony of gray short-tailed opossums, small, Brazilian marsupials. At 24 degrees C, both males and females used more nesting material and built larger nests of better quality than at 27 degrees C. Although both males and females built nests using the mouth, forelegs, hindlegs and tail, females built nests more reliably at the higher temperature and used more nesting material than males at both temperatures. These findings are discussed with respect to the thermoregulatory and reproductive characteristics of marsupials.

Estrogen in peripheral plasma during postnatal development in gray short-tailed opossums

Plasma samples obtained from gray short-tailed opossums (Monodelphis domestica) at selected ages through adulthood were assayed for estrogen (E). Levels of E in one mixed-sex plasma pool of animals aged postnatal day (pd) 4 and one of two mixed-sex plasma pools of animals aged pd 8 were over 300 pg/ml. On pd 16, E levels in males and females averaged 30 and 47 pg/ml, respectively. While no significant sex differences in E levels were seen on pd 30 or pd 60, mean E levels for animals on pd 30 were significantly higher (275 pg/ml in males and 181 pg/ml in females) than on pd 60 (78 pg/ml in males and 85 pg/ml in females) or pd 145 (adults). In adult animals, estrogen levels in females averaged 54 pg/ml; all adult male E levels were below the limit of sensitivity of the assay. Maternal E levels, which did not vary significantly by age of litter, averaged 10 pg/ml overall. These findings are discussed with respect to possible significance of high E levels in developing marsupials for sexual differentiation and general brain development.

Neural aromatase activity in a marsupial, the gray short-tailed opossum (Monodelphis domestica): ontogeny during postnatal development and androgen regulation in adulthood

Neural aromatase activity (AA) was measured in gray short-tailed opossums (Monodelphis domestica) on the day of birth and at selected ages through adulthood. In adulthood, regulation by testicular androgens was examined. In mixed-sex samples of whole brain, AA was present at birth and increased until postnatal day (PD) 16. In hypothalamus-preoptic area (HPOA), significantly higher levels of AA were seen in animals on PD 16 than on PD 30 and PD 30 animals had higher levels of AA than all older ages including adults. Significant sex differences in HPOA AA with male levels higher, were seen only on PD 16 and in adulthood. While lower overall than in HPOA, AA was present also in cerebral cortex (CX). In CX, AA was higher on PDs 16 and 30 than at older ages. Significant sex differences in CX AA were observed only in adulthood. One week following castration in adulthood, AA dropped significantly in CX but not in HPOA. These findings are compared with those obtained from other marsupial and eutherian mammals with reference to the possible significance of AA in sexual differentiation of the gray opossum brain.

Estrogen binding macromolecules in hypothalamus―preoptic area of male and female gray short-tailed opossums (Monodelphis domestica)

Estrogen binding sites in the hypothalamus-preoptic area (HPOA) of adult male and female gray opossums (Monodelphis domestica) were characterized by incubating cytosols from gonadectomized animals with [3H]estradiol (E2) in the presence or absence of excess unlabeled E2. Scatchard analyses revealed that HPOA cytosols from both males and females contained high concentrations (25-30 fmol/mg protein) of binding sites with a very high affinity (Kd = 0.08-0.12 nM) for E2. There was no sex difference in either the number or the affinity of estrogen binding sites. Competition studies indicated that estrogen agonists and antagonists displaced [3H]E2 from binding sites in HPOA cytosols more effectively than did progestins, androgens, or adrenal corticoids. These data suggest that the HPOA of both male and female gray opossums contains macromolecules with several of the steroid binding properties characteristic of estrogen receptors.

Ontogeny of estrogen binding sites in the brain of gray short-tailed opossums (Monodelphis domestica).

This study evaluated the binding of [3H]estradiol to brain cytosols from gray short-tailed opossums ranging in age from newborn to 63 days postnatal. Estrogen binding was undetectable in whole-brain cytosol of newborn opossums. By postnatal day 4, high affinity (Kd = 0.2 nM), saturable (Bmax = 3 fmol/mg protein) estrogen binding sites were present, and estrogen binding in whole-brain remained low but detectable (2-5 fmol/mg) through day 63. In contrast, estrogen binding sites in the hypothalamus-preoptic area increased substantially from 3.4 fmol/mg on day 16 to 14 fmol/mg by day 63.

The luteinizing hormone-releasing hormone (LH-RH) system in neonatally estradiol-feminized male gray short-tailed opossums (Monodelphis domestica)

In this study, the luteinizing hormone-releasing hormone (LH-RH) system was examined in adult gray short-tailed opossums that had been treated with estradiol benzoate (EB) on days 1 and 3 of postnatal life, a treatment which results in complete block of testicular development. The finding that the organization of the LH-RH system in neonatally EB-treated males did not differ from that of neonatally untreated animals indicates that the LH-RH system can develop normally despite the absence of gonads throughout perinatal life.