Barbara Lanza

Measurement of kinaesthetic properties of in-brine table olives by microstructure of fracture surface, sensory evaluation and texture profile analysis (TPA)

BACKGROUND A series of transformations occur in olive fruit both during ripening and processing. In particular, significant changes in the microstructural composition affect the flavour, texture, nutrients and overall quality of the end product. Texture is one of the sensory quality attributes of greatest importance to consumer acceptance. In the present work, kinaesthetic properties of in-brine table olives of three cultivars of Olea europaea L. (Bella di Cerignola, Peranzana and Taggiasca cvs) were provided by several measurements of olive tissue texture by sensory, rheological and microstructural approaches. RESULTS Olives at the same stage of ripening and processed with the same technology, but belonging to different cultivars, showed significant differences at microstructural, sensorial and rheological levels. To describe the relationship between the three variables, multiple regression analysis and principal component analysis were chosen. CONCLUSION Differences in microstructure were closely related both in terms of hardness measured by texture profile analysis and hardness measured by sensory analysis. The information provided could be an aid for screening and training of a sensory panel.

Control of Aspergillus carbonarius in grape berries by Lactobacillus plantarum: A phenotypic and gene transcription study

The in vitro and in situ antifungal activity of Lactobacillus plantarum against the ochratoxigenic fungus Aspergillus carbonarius was investigated in this study. Four different fungal isolates from grape berries were co-cultured with four different strains of L. plantarum on Malt Extract Agar (MEA) plates at 30 °C. Bacterial strains inhibited fungal growth up to 88% and significantly reduced toxin production up to 100%. In addition, L. plantarum was evaluated as biocontrol agent against A. carbonarius growth and OTA production on table grapes. Temporal studies of bacterial antagonism were performed with two different grape cultivars. Artificially wounded and unwounded berries were pre-treated with 108 CFU/mL bacteria and inoculated with 106 spores/mL of A. carbonarius ochratoxigenic isolates. Biocontrol agents displayed high rate of colonization on grapes during 5 days of incubation at 30 °C. Scanning electron microscopy (SEM) also determined the presence of microorganisms on grape surface. Bacterial strains were effective in controlling fungal infection reaching up to 71% inhibition rates. However the presence of wounds on grape skin facilitated infection of berries by A. carbonarius, since unwounded berries showed lower levels of infection. Results also revealed significant reduction in mycotoxin production ranging between 32% and 92%. Transcriptome analysis following exposure to co-cultivation, exhibited differential expression for each gene studied of AcOTAnrps (Aspergillus carbonarius OTA nonribosomal), AcOTApks (Aspergillus carbonarius OTA polyketide synthase) and laeA, emphasizing the significance of strain variability. The genes AcOTAnrps and laeA were most influenced by the presence of L. plantarum. This work is a contribution for the potential biocontrol of toxigenic fungi in table grapes by lactic acid bacteria (LAB). The above findings underline the significance of bacterial strain variability on the effectiveness of biopreservative features of L. plantarum strains.