Barbara Mulinacci | Researchain

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Featured researches published by Barbara Mulinacci.

Journal of Neuroimmunology | 2011

IgG and IgM antibodies to the refolded MOG1–125 extracellular domain in humans

Francesca Gori; Barbara Mulinacci; Lara Massai; Carlo Avolio; Mariantonietta Caragnano; Elisa Peroni; S. Lori; Mario Chelli; Anna Maria Papini; Paolo Rovero; Francesco Lolli

Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.

Proceedings of the National Academy of Sciences of the United States of America | 2005

An N-glucosylated peptide detecting disease-specific autoantibodies, biomarkers of multiple sclerosis

Francesco Lolli; Barbara Mulinacci; Alfonso Carotenuto; Bruno Bonetti; Giuseppina Sabatino; Benedetta Mazzanti; Anna Maria D'Ursi; Ettore Novellino; Marta Pazzagli; Laura Lovato; Maria Claudia Alcaro; Elisa Peroni; Maria C. Pozo-Carrero; Francesca Nuti; Luca Battistini; Giovanna Borsellino; Mario Chelli; Paolo Rovero; Anna Maria Papini

Letters in Peptide Science | 2002

Assessment of new 6-Cl-HOBt based coupling reagents for peptide synthesis. Part 1: Coupling efficiency study

Giuseppina Sabatino; Barbara Mulinacci; Maria Claudia Alcaro; Mario Chelli; Paolo Rovero; Anna Maria Papini

Journal of Medicinal Chemistry | 2006

Conformation−Activity Relationship of Designed Glycopeptides as Synthetic Probes for the Detection of Autoantibodies, Biomarkers of Multiple Sclerosis

Alfonso Carotenuto; Anna Maria D'Ursi; Barbara Mulinacci; Ilaria Paolini; Francesco Lolli; Anna Maria Papini; Ettore Novellino; Paolo Rovero

Biopolymers | 2003

MS PepKit, the first diagnostic test based on CSF114(Glc) a glycopeptide to follow-up Multiple Sclerosis.

Anna Maria Papini; Barbara Mulinacci; Elisa Peroni; Francesca Nuti; Giuseppina Sabatino; Maria C. Pozo-Carrero; Benedetta Mazzanti; Marta Pazzagli; Luca Massacesi; Mario Chelli; Paolo Rovero; Francesco Lolli

Journal of Neuroimmunology | 2004

A proteomic approach to characterize a native antigen involved in autoantibody response in Multiple Sclerosis by a structure-based designed glycopeptide.

Benedetta Mazzanti; Marta Pazzagli; Maria Claudia Alcaro; Barbara Mulinacci; Elisa Peroni; Francesca Nuti; Giuseppina Sabatino; M. C. Pozo Carrero; Alfonso Carotenuto; Bruno Bonetti; Luca Battistini; Diego Franciotta; Stefano Sotgiu; Roberta Lanzillo; Mario Chelli; Paolo Rovero; Francesco Lolli; Anna Maria Papini

Advances in Experimental Medicine and Biology | 2009

Semi-Synthetic Strategies to Obtain Glucosylated MOG to Identify Antibodies as Biomarkers in Multiple Sclerosis Disease

Francesca Gori; Barbara Mulinacci; Eva Bonchi; Mario Chelli; Francesco Lolli; Anna Maria Papini; Paolo Rovero

Advances in Experimental Medicine and Biology | 2009

A glycopeptide-based technique for selective antibody purification.

Elisa Peroni; Marta Tontini; Barbara Mulinacci; Mario Chelli; Francesco Lolli; Paolo Rovero; Anna Maria Papini

Advances in Experimental Medicine and Biology | 2009

Studies for identification of the minimal epitope(s) mimicked by the synthetic glucopeptide CSF114(Glc).

Francesca Nuti; Barbara Mulinacci; Elisa Peroni; Maria Claudia Alcaro; Ilaria Paolini; Francesco Benedetti; Alfonso Carotenuto; Francis Ciolli; Francesco Lolli; Mario Chelli; Paolo Rovero; Anna Maria Papini

Journal of Peptide Science | 2004

Beta-hairpin glycopeptides as mimetic autoantigens in Multiple Sclerosis

Barbara Mulinacci; Ilaria Paolini; Alfonso Carotenuto; Benedetta Mazzanti; Marta Pazzagli; Mario Chelli; Francesco Lolli; Paolo Rovero; Anna Maria Papini

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