Barbara N. Ngwenya

Shocks and rural livelihoods in the Okavango Delta, Botswana

This paper describes the impacts that three shocks in the Okavango Delta, Botswana, have had on rural livelihoods: the desiccation of river channels, animal diseases, and HIV/AIDS. Primary data was collected from five study areas, using formal questionnaire interviews and focus group discussions. The paper reveals the adverse effects on rural livelihoods. It describes the way households have been exposed to poverty and vulnerability and the various ways they have coped or adapted, such as by re-allocating their labour, liquidating their assets to cover medical expenses and funeral costs, reducing the area ploughed for crops, hiring labour, digging wells and switching from flood recession agriculture to dryland farming. The Botswana government has provided safety nets to help households cope, but this paper recommends that peoples responses to these shocks should be taken into account in future policy and programme formulation.

Determination of potentially toxic heavy metals in traditionally used medicinal plants for HIV/AIDS opportunistic infections in Ngamiland District in Northern Botswana.

The determination of four potentially toxic heavy metals, arsenic, chromium, lead and nickel in twelve plant species used for the treatment of perceived HIV and AIDS-associated opportunistic infections by traditional healers in Ngamiland District in Northern Botswana, a metal mining area, was carried out using atomic absorption spectrometry. The medicinal plants; Dichrostachys cinerea, Maerua angolensis, Mimusops zeyheri, Albizia anthelmintica, Plumbago zeylanica, Combretum imberbe, Indigofera flavicans, Clerodendrum ternatum, Solanum panduriforme, Capparis tomentosa, Terminalia sericea and Maytenus senegalensis contained heavy metals in varying quantities: arsenic 0.19-0.54 μg g(-1), chromium 0.15-1.27 μg g(-1), lead 0.12-0.23 μg g(-1) and nickel 0.09-0.21 μg g(-1) of dry weight. Chromium was found to be the most abundant followed by arsenic and lead. Nickel was undetectable in nine plant species. M. senegalensis contained the largest amounts of arsenic, chromium and lead. All metals determined were below the WHO permissive maximum levels. The possible maximum weekly intakes of the heavy metals following treatment regimes were insignificant compared to the provisional tolerable weekly intake levels recommended by WHO and the Joint FAO/WHO Expert Committee on Food Additives. This suggests that heavy metal exposure to patients originating from consumption of traditional medicinal plant preparations is within non health-compromising limits.

Old plants newly discovered: Cassia sieberiana D.C. and Cassia abbreviata Oliv. Oliv. root extracts inhibit in vitro HIV-1c replication in peripheral blood mononuclear cells (PBMCs) by different modes of action.

ETHNOPHARMACOLOGICAL RELEVANCE Despite advances in anti-retroviral therapy which has transformed HIV/AIDS from a fatal to a manageable chronic disease, increasing viral drug resistance, side effects and uneven access to anti-retroviral drugs remain considerable therapeutic challenges. Partly as a consequence of these shortcomings and partly based on the fact that HIV/AIDS gives rise to opportunistic infections whose symptoms have been managed in Africa in an HIV/AIDS-independent context by traditional healers for centuries, many HIV/AIDS patients use herbal medicines. The aim of this study was to screen selected medicinal plants from Botswana, used by traditional healers to treat/manage HIV/AIDS, for inhibitory activities on HIV replication. MATERIALS AND METHODS Based on an ethnomedical survey, ethanolic tannin-containing and tannin-free extracts from 10 medicinal plants were tested for inhibitory properties against a clone of HIV-1c (MJ(4)) measuring cytopathic effect protection and levels of viral p24 antigen in infected PBMCs. RESULTS Cassia sieberiana D.C., Cassia abbreviata Oliv. Oliv. and Plumbago zeylanica L. extracts showed significant inhibition of HIV-1c (MJ(4)) replication. The inhibitory activity of the Plumbago zeylanica extract could be attributed to its tannin content. Anti-HIV activity of Cassia sieberiana root and bark extracts, and Cassia abbreviata root extracts occurred in a concentration-dependent manner with an effective concentration (EC(50)) of 65.1μg/ml, 85.3μg/ml and 102.8μg/ml, respectively. Experiments to elucidate possible mechanism(s) of action revealed that Cassia sieberiana root and bark extracts blocked HIV replication at its binding- (EC(50)=70.2μg/ml and 90.8μg/ml, respectively) and entry stage (EC(50)=88.9μg/ml and 100.5μg/ml, respectively) while Cassia abbreviata extracts did not. CONCLUSIONS We report here for the first time a direct inhibitory effect on HIV-1c replication of extracts from two extremely popular medicinal plants, Cassia sieberiana and Cassia abbreviata. Considering the traditional uses of both Cassia species, our findings strongly suggest pilot clinical observational studies involving traditional healers to further evaluate the therapeutic potential of the Cassia extracts.

Clinical Malaria Transmission Trends and Its Association with Climatic Variables in Tubu Village, Botswana: A Retrospective Analysis

Good knowledge on the interactions between climatic variables and malaria can be very useful for predicting outbreaks and preparedness interventions. We investigated clinical malaria transmission patterns and its temporal relationship with climatic variables in Tubu village, Botswana. A 5-year retrospective time series data analysis was conducted to determine the transmission patterns of clinical malaria cases at Tubu Health Post and its relationship with rainfall, flood discharge, flood extent, mean minimum, maximum and average temperatures. Data was obtained from clinical records and respective institutions for the period July 2005 to June 2010, presented graphically and analysed using the Univariate ANOVA and Pearson cross-correlation coefficient tests. Peak malaria season occurred between October and May with the highest cumulative incidence of clinical malaria cases being recorded in February. Most of the cases were individuals aged >5 years. Associations between the incidence of clinical malaria cases and several factors were strong at lag periods of 1 month; rainfall (r = 0.417), mean minimum temperature (r = 0.537), mean average temperature (r = 0.493); and at lag period of 6 months for flood extent (r = 0.467) and zero month for flood discharge (r = 0.497). The effect of mean maximum temperature was strongest at 2-month lag period (r = 0.328). Although malaria transmission patterns varied from year to year the trends were similar to those observed in sub-Saharan Africa. Age group >5 years experienced the greatest burden of clinical malaria probably due to the effects of the national malaria elimination programme. Rainfall, flood discharge and extent, mean minimum and mean average temperatures showed some correlation with the incidence of clinical malaria cases.

Assessment of Risk Factors Associated with Malaria Transmission in Tubu Village, Northern Botswana

This study investigated potential risk factors associated with malaria transmission in Tubu village, Okavango subdistrict, a malaria endemic area in northern Botswana. Data was derived from a census questionnaire survey, participatory rural appraisal workshop, field observations, and mosquito surveys. History of malaria episodes was associated with several factors: household income (P < 0.05), late outdoor activities (OR = 7.016; CI = 1.786–27.559), time spent outdoors (P = 0.051), travel outside study area (OR = 2.70; CI = 1.004–7.260), nonpossession of insecticide treated nets (OR = 0.892; CI = 0.797–0.998), hut/house structure (OR = 11.781; CI = 3.868–35.885), and homestead location from water bodies (P < 0.05). No associations were established between history of malaria episodes and the following factors: being a farmer (P > 0.05) and number of nets possessed (P > 0.05). Eave size was not associated with mosquito bites (P > 0.05), frequency of mosquito bites (P > 0.05), and time of mosquito bites (P > 0.05). Possession of nets was very high (94.7%). Close proximity of a health facility and low vegetation cover were added advantages. Some of the identified risk factors are important for developing effective control and elimination strategies involving the community, with limited resources.

People Living With HIV and AIDS on the Brink: Stigma—A Complex Sociocultural Impediment in the Fight Against HIV and AIDS in Botswana

HIV-related stigma is a life-altering phenomenon. The consequence of the stigmatization process sets apart stigmatized person(s) as a distinct category, leading to various forms of disapproval, rejection, exclusion, labeling, stereotyping, and discrimination. Stigma of HIV-positive people in Botswana is a complex social phenomenon associated with the disease itself and the behaviors that lead to infection. This is a synthesis paper based on the literature review on HIV- and AIDS-related stigmatization of HIV-positive people in Botswana and in-depth interviews with people living with HIV and AIDS (PLWHAs). I examine the literature on HIV- and AIDS-related stigmatization and subsequent discrimination and the implications for intervention programs for people living with HIV and AIDS. The findings from the literature and in-depth interviews show that HIV–AIDS-related stigma is deeply embedded in societal structures and culture which promote nonacceptance of those branded HIV positive. This often is reinforced at a practical level by pervasive negative attitudes toward PLWHAs. Recommendations argue for the adoption of Healthy Relationship. This intervention seeks to promote and strengthen decision-making skills among PLWHAs and programs that promote destigmatization of, and tolerant attitudes toward, PLWHAs.

Climate Variability and Rural Livelihoods: How Households Perceive and Adapt to Climatic Shocks in the Okavango Delta, Botswana

AbstractClimate variability and change have adverse effects on agricultural production and other livelihood strategies of the rural households. The paper hypothesizes that rural households naturally devise means of overcoming the challenges currently posed by climate variability. The research article addresses the question of how rural households apply local knowledge of weather forecasting in adapting to climate variability in the Okavango Delta. It specifically probes, among others, the extent to which climate variability has affected agricultural production over the last 10 years in the area. A multistage sampling procedure was used to select a total of 592 households from eight rural communities. Key informant interviews, focus group discussions, and a stakeholder workshop were used to obtain demographic, socioeconomic, psychosocial, and climatic information. Households used both natural animate and inanimate indicators to predict the weather. To enhance household adaptation to climatic events, indige...

Threats to maternal and child well-being in rural communities in Ngamiland, Botswana.

The authors’ main objective of the study was to explore threats to maternal and child well-being in two nonriparian and two riparian communities in the east and west of the Okavango River Basin in Ngamiland District, Botswana. Primary data were collected from a simple random sample of 60 households. Data on womens access to human, financial, physical and institutional capital, productive assets and livelihood activities, household food resources, and social services (education, health, water, sanitation) were collected. Childrens nutritional status was also evaluated. Our observations reveal that caregiving is gender biased toward women whose well-being is undermined by their limited access to resources. Further, womens poor access to basic household resources undermines gains made through clinic-based maternal and child welfare nutrition programs. In addition, a significant proportion of childrens caregivers have access to low-tier primary health care provision in the study area, but inadequate physical infrastructural development limits their access to specialized care, particularly emergency obstetric services. Childhood malnutrition is also a concern in the study area, and it mostly affects children whose caregivers have limited access to resources. The authors conclude that improving maternal and child access to education and health services remains a challenge in the district. The authors recommend interventions that prioritize sensitive aspects of child well-being, early childhood education, and development.

Potential Impacts of Biofuel Development on Biodiversity in Chobe District, Botswana

There is a need to use cleared idle agricultural land for biofuel production in order to avoid adverse impacts on food security and biodiversity. This chapter examines the potential impacts of biofuel development on biodiversity in Chobe District, Botswana, using literature review and stakeholder interviews. The stakeholders interviewed confirmed that there are significant areas of idle agricultural land available in the district, but most of it is not cleared. Therefore, the production of biofuels in Chobe District may on the one hand negatively affect biodiversity through the clearing of new land, but on the other hand it may not adversely affect food security since idle agricultural land will be used. The use of marginal land for biofuel production may also harm biodiversity (plant and animal species). This chapter shows that the use of jatropha and sweet sorghum for biofuel production is likely to have a lower impact on biodiversity compared to corn. In conclusion, research on biology, chemistry as well as agronomic aspects of energy crops should be undertaken prior to large-scale biofuel development in Botswana.

“Now I Heal with Pride”—The Application of Screens-to-Nature Technology to Indigenous Knowledge Systems Research in Botswana: Implications for Drug Discovery

The combination of indigenous knowledge systems (IKS) with techniques of modern drug discovery platforms has a great potential to overcome the current innovation deficits in drug discovery. We describe the adaptation of a set of field-suitable bioassays, the “Screens-to-Nature” (STN) system, to a participatory research tool suitable for overcoming some of the difficulties in establishing cooperation between indigenous knowledge holders and scientists. Extracts from 621 plant samples representing 214 species from 71 plant families in Botswana have been qualitatively screened for antibacterial, antifungal, and antiprotozoal activities, as well as for α-glucosidase and protease inhibitory properties. The results provide a first bioactivity profile of medicinal plants in Botswana. Close to half of the samples (47%) were provided by traditional healers and community members from two regions in Botswana, the Kweneng and Ngamiland Districts. Screening results were consistently shared and discussed with indigenous knowledge holders who participated in the STN project. A survey conducted among 28 traditional healers revealed that a large majority (93%) perceived the STN approach as beneficial to indigenous knowledge holders and expressed their desire to continue contributing to natural product research. In that way, the STN approach provided a basis for the establishment of an indigenous knowledge-guided drug discovery platform in Botswana.